Volume 14, Issue 2 (Iranian South Medical of Journal 2011)                   Iran South Med J 2011, 14(2): 76-83 | Back to browse issues page

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Kargar M, Daneshvar M, Homayoun M. Surveillance of Virulence Markers and Antibiotic Resistance of Shiga toxin Producing E.coli O157:H7 Strains from Meats Purchase in Shiraz. Iran South Med J 2011; 14 (2) :76-83
URL: http://ismj.bpums.ac.ir/article-1-253-en.html
1- Islamic Azad University of Jahrom , mkargar@jia.ac.ir
2- Islamic Azad University of Jahrom
Abstract:   (18018 Views)

Background: Shiga toxin Producing Escherichia coli O157:H7 is a common pathogen in cattle, which occasional causes some human disease. This bacterium can potentially contaminate meat and clinical cases of E.coli O157:H7 infections are often associated with consumption of undercooked ground beef. Methods: In this cross-sectional study 122 samples of ground meat were collected and after enrichment in specific culture media and evaluation sorbitol fermentation and their β-glucoronidase activity, the isolation of E.coli O157:H7 strains have been confirmed with specific antisera. Then virulence genes verotoxin, intimin and hemolysin with multiplex PCR and antibiotic resistance strains with disk diffusion method have been tested. Results: Out of specimens that have been supplied, 119 sorbitol negative colonies isolated which 3 strains O157:H7 (2.45%) with specific antisera confirmed. Out of considered virulence genes, in two cases of these samples (1.64%) the stx1 and eaeA genes were seen and also 2 isolated bacteria had resistance to erythromycin, tetracycline, ampicillin, penicillin, clindamicin, cefixime, novobiocin, and gentamicin antibiotics. Conclusion: As this organism lives in intestines of healthy cattle, preventive measures on cattle farms and during meat processing are necessary.

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Type of Study: Original | Subject: General
Received: 2010/01/7 | Accepted: 2010/02/16 | Published: 2011/03/15

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