Volume 20, Issue 5 (Iranian South Medical Journal 2017)
Iran South Med J 2017, 20(5): 416-425 |
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Mazoochi T, Karimian M, Mazoochi M, Hosseinzadeh Colagar A. Association of c.*49T>C and c.-932 G>A Polymorphisms of CYBA Gene with Coronary Artery Disease: a Case-Control Study in Kashan Population. Iran South Med J 2017; 20 (5) :416-425
URL: http://ismj.bpums.ac.ir/article-1-895-en.html
URL: http://ismj.bpums.ac.ir/article-1-895-en.html
1- Anatomical Sciences Research Center, Kashan University of Medical Sciences, Kashan, Iran
2- Anatomical Sciences Research Center, Kashan University of Medical Sciences, Kashan, Iran
Department of Molecular and Cell Biology, School of Basic Sciences, University of Mazandaran, Babolsar, Iran
3- Department of Cardiology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran
4- Department of Molecular and Cell Biology, School of Basic Sciences, University of Mazandaran, Babolsar, Iran
2- Anatomical Sciences Research Center, Kashan University of Medical Sciences, Kashan, Iran
Department of Molecular and Cell Biology, School of Basic Sciences, University of Mazandaran, Babolsar, Iran
3- Department of Cardiology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran
4- Department of Molecular and Cell Biology, School of Basic Sciences, University of Mazandaran, Babolsar, Iran
Abstract: (4745 Views)
Background: The CYBA (Cytochrome B-245 Alpha Chain; p22phox) gene encodes an essential subunit of NADH/NADPH-oxidase. This enzyme is expressed in smooth muscle cells of arteries which produces several active oxygen products. On the other hand, oxidative stress has a significant role in the pathogenesis of coronary artery disease (CAD). This study aimed to investigate the association between rs7195830 (c.*49T>C) and rs9932581 (c.-930G>A) polymorphisms in CYBA gene with coronary artery disease in an Iranian population.
Materials and Methods: In this case-control study, citrated blood samples were obtained from 180 participants, including 85 patient with CAD and 95 healthy volunteers. The fragments containing rs7195830 and rs9932581 of CYBA gene from extracted genome were amplified by polymerase chain reaction. Then the genotype of samples was detected using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Genetic association analysis was performed using logistic regression test.
Results: Data analysis of c.-930G>A polymorphism revealed a significant association between AA genotype and risk of CAD (OR: 2.71, 95%CI: 1.04-7.06, p= 0.041). Also, the allelic analysis revealed that there was a significant association between A allele and CAD risk (OR: 1.65, 95%CI: 1.05-2.57, p=0.029), while there was no significant association between c.*49T>C polymorphism and risk of CAD.
Conclusion: The study results showed that the c.-930G>A polymorphism might be associated with the susceptibility to CAD and could be considered as a potential biomarker in future studies.
Materials and Methods: In this case-control study, citrated blood samples were obtained from 180 participants, including 85 patient with CAD and 95 healthy volunteers. The fragments containing rs7195830 and rs9932581 of CYBA gene from extracted genome were amplified by polymerase chain reaction. Then the genotype of samples was detected using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Genetic association analysis was performed using logistic regression test.
Results: Data analysis of c.-930G>A polymorphism revealed a significant association between AA genotype and risk of CAD (OR: 2.71, 95%CI: 1.04-7.06, p= 0.041). Also, the allelic analysis revealed that there was a significant association between A allele and CAD risk (OR: 1.65, 95%CI: 1.05-2.57, p=0.029), while there was no significant association between c.*49T>C polymorphism and risk of CAD.
Conclusion: The study results showed that the c.-930G>A polymorphism might be associated with the susceptibility to CAD and could be considered as a potential biomarker in future studies.
Type of Study: Original |
Subject:
Cardiovascular System
Received: 2016/10/29 | Accepted: 2017/03/13 | Published: 2017/11/13
Received: 2016/10/29 | Accepted: 2017/03/13 | Published: 2017/11/13
References
1. Shafiei E, Fouladi Z, Shafiei P. The safe interval time of crystalloid cardioplegic solution transfusion during CABG. Iran South Med J. 2015; 18(1) :71-79.
2. Sowers JR, Epstein M, Frohlich ED. Diabetes, hypertension, and cardiovascular disease an update. Hypertension 2001; 37(4): 1053-9. [DOI:10.1161/01.HYP.37.4.1053]
3. Pjanic M, Miller CL, Wirka R, et al. Genetics and genomics of coronary artery disease. Curr Cardiol Rep 2016; 18(10): 102. [DOI:10.1007/s11886-016-0777-y]
4. Madamanchi NR, Vendrov A, Runge MS. Oxidative stress and vascular disease. Arterioscler Thromb Vasc Biol 2005; 25(1): 29-38. [DOI:10.1161/01.ATV.0000150649.39934.13]
5. Qin B, Yang H, Xiao B, et al. Role of microRNAs in endothelial inflammation and senescence. Mol Biol Rep 2012; 39(4): 4509-18. [DOI:10.1007/s11033-011-1241-0]
6. Konior A, Schramm A, Czesnikiewicz-Guzik M, et al. NADPH oxidases in vascular pathology. Antioxid Redox Signal 2014; 20(17): 2794-814. [DOI:10.1089/ars.2013.5607]
7. Paravicini TM, Touyz RM. NADPH oxidases, reactive oxygen species, and hypertension clinical implications and therapeutic possibilities. Diabetes Care 2008; 31 Suppl 2: S170-80. [DOI:10.2337/dc08-s247]
8. Kikuchi H, Hikage M, Miyashita H, et al. NADPH oxidase subunit, gp91(phox) homologue, preferentially expressed in human colon epithelial cells. Gene 2000; 254(1-2): 237-43. [DOI:10.1016/S0378-1119(00)00258-4]
9. Uenno N, Takeya R, Miyano K, et al. The NADPH oxidase Nox3 constitutively produces superoxide in a p22phox-dependent manner: its regulation by oxidase organizers and activators. J Biol Chem 2005; 280(24): 23328-39. [DOI:10.1074/jbc.M414548200]
10. Mohazzab KM, Kaminski PM, Wolin MS. NADH oxidoreductase is a major source of superoxide anion in bovine coronary artery endothelium. Am J Physiol 1994; 266(6 Pt 2): H2568-72.
11. Sorescu D, Weiss D, Lassègue B, et al. Superoxide production and expression of nox family proteins in human atherosclerosis. Circulation 2002; 105(12): 1429-35. [DOI:10.1161/01.CIR.0000012917.74432.66]
12. Liang B, Wei Q, Shen T, et al. The A640G polymorphism in the NAD (P) H oxidase p22phox gene (CYBA) is associated with risk reduction of coronary heart disease: a meta-analysis. Clin Biochem 2014; 47(6):409-16 [DOI:10.1016/j.clinbiochem.2013.12.001]
13. Najafi M, Alipoor B, Shabani M, et al. Association between rs4673 (C/T) and rs13306294 (A/G) haplotypes of NAD (P) H oxidase p22phox gene and severity of stenosis in coronary arteries. Gene 2012; 499(1): 213-7. [DOI:10.1016/j.gene.2012.02.032]
14. Hu P, Huang MY, Hu XY, et al. Meta-analysis of C242T polymorphism in CYBA genes: risk of acute coronary syndrome is lower in Asians but not in Caucasians. J Zhejiang Univ Sci B 2015; 16(5): 370-9. [DOI:10.1631/jzus.B1400241]
15. Green MR, Sambrook J. Molecular Cloning: a Laboratory Manual. 4th ed. New York: Cold Spring Harbor Laboratory Press, 2012, 104-111
16. Pollock A, Jones DS. Coronary artery disease and the contours of pharmaceuticalization. Soc Sci Med 2015; 131: 221-7. [DOI:10.1016/j.socscimed.2014.06.035]
17. Raygan F, Karimian M, Rezaeian A, et al. Angiotensinogen-M235T as a risk factor for myocardial infarction in Asian populations: a genetic association study and a bioinformatics approach. Croat Med J 2016; 57(4): 351-62. [DOI:10.3325/cmj.2016.57.351]
18. Mazaheri M, Karimian M, Behjati M, et al. Association analysis of rs1049255 and rs4673 transitions in p22phox gene with coronary artery disease: A case-control study and a computational analysis. Ir J Med Sci 2017; 1-8. [DOI:10.1007/s11845-017-1601-4]
19. McClelland RL, Jorgensen NW, Budoff M, et al. 10-year coronary heart disease risk prediction using coronary artery calcium and traditional risk factors: derivation in the MESA (Multi-Ethnic Study of Atherosclerosis) with validation in the HNR (Heinz Nixdorf Recall) study and the DHS (Dallas Heart Study). J Am Coll Cardiol 2015; 66(15): 1643-53. [DOI:10.1016/j.jacc.2015.08.035]
20. Niemiec P, Nowak T, Iwanicki T, et al. The -930A> G polymorphism of the CYBA gene is associated with premature coronary artery disease. A case–control study and gene-risk factors interactions. Mol Bio Rep 2014; 41(5): 3287-94. [DOI:10.1007/s11033-014-3191-9]
21. Nowak T, Niemiec P, Górczyńska-Kosiorz S, et al. The CYBA gene 49A> G polymorphism (rs7195830) is associated with hypertension in patients with coronary artery disease. Biomed Res Int 2016; 2016: 1539671. [DOI:10.1155/2016/1539671]
22. Zalba G, San José G, Moreno MU, et al. NADPH oxidase-mediated oxidative stress: genetic studies of the p22phox gene in hypertension. Antioxid Redox Signal 2005; 7(9-10): 1327-36. [DOI:10.1089/ars.2005.7.1327]
23. Zalba G, Beaumont FJ, San José G, et al. Vascular NADH/NADPH oxidase is involved in enhanced superoxide production in spontaneously hypertensive rats. Hypertension 2000; 35(5): 1055-61. [DOI:10.1161/01.HYP.35.5.1055]
24. Karimian M, Hosseinzadeh Colagar A. Human MTHFR-G1793A transition may be a protective mutation against male infertility: a genetic association study and in silico analysis. Hum Fertil 2017; 1-9.
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