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:: Volume 23, Issue 2 (Iranian South Medical Journal 2020) ::
Iran South Med J 2020, 23(2): 99-107 Back to browse issues page
Magnetic Separation of Giardia Cysts from Water
Reza Ghasemikhah1 , Zainab Davudi Moghadam2, Fatemeh Ahmadi2, Mahsa Rasulpur2, Mohammad Reza Bayatiani3, Reza Hajihossein1, Hossein Sarmadian4, Fatemeh Seif 5
1- Department of Parasitology and Mycology, School of Medicine, Arak University of Medical Sciences, Arak, Iran
2- Students Research Committee, School of Paramedical Sciences, Arak University of Medical Sciences, Arak, Iran
3- Department of Radiotherapy and Medical Physics, School of Paramedical Sciences, Arak University of Medical sciences and Khansari Hospital, Arak, Iran
4- Department of Infectious Disease, School of Medicine, Arak University of Medical Sciences, Arak, Iran
5- Department of Radiotherapy and Medical Physics, School of Paramedical Sciences, Arak University of Medical sciences and Khansari Hospital, Arak, Iran , seif@arakmu.ac.ir
Abstract:   (1054 Views)
Background: Giardia is one of the infectious agents that can be transmitted by surface water and cause a disease. We designed and fabricated a magnetic system to separate the giardia cysts from water.
Materials and Methods: In this study, giardia cysts were concentrated by sucrose floatation method. The cysts were mixed with physiological saline and their concentration was adjusted to 100,000 cysts per cubic millimeter using Neubauer slide. The isolated cysts were passed through the magnetic field generated by the 200-msT magnets. The cysts were attracted to the magnet and removed from the suspension. The cysts were exposed to magnetic field for one week in order to evaluate the effect of magnetic field on their survival, which was reported as 0.1% using eosin staining under a light microscope.
Results: The results showed that magnetic field can relatively absorb giardia cysts from water, the survival rate of the cysts did not change significantly in different days (P≥0.05).
Conclusion: The designed magnetic system can be reinforced to isolate giardia cysts, which is economical and has no chemical effects on water.
Keywords: Giardia, cyst separation, Sucrose, Magnetic field
Full-Text [PDF 1016 kb]   (238 Downloads)    
Type of Study: Original | Subject: Parasitology
Received: 2019/10/28 | Accepted: 2020/02/5 | Published: 2020/06/27
1. Thompson RC, Monis PT. Variation In Giardia: Implications For Taxonomy And Epidemiology. Adv Parasitol 2004; 58(95): 69-137. [DOI:10.1016/S0065-308X(04)58002-8]
2. Vanni I, Caccio SM, Van Lith L, et al. Detection Of Giardia Duodenalis Assemblages A And B In Human Feces By Simple, Assemblage-Specific PCR Assays. PLoS Negl Trop Dis 2012; 6(8): e1776. [DOI:10.1371/journal.pntd.0001776]
3. Abasian L, Talebi Fd, Medicine Faculty, et al. Prevalence Of Giardia Lamblia In Iran: A Systematic Review And Meta Analysis Study. Res Med 2013; 36(5): 111-6. (Persian)
4. Eckmann L. Mucosal Defences Against Giardia. Parasite Immunol 2003; 25(5): 259-70. [DOI:10.1046/j.1365-3024.2003.00634.x]
5. Mahmoudi MR, Nazemalhosseini-Mojarad E, Karanis P. Genotyping Of Giardia Lamblia And Entamoeba Spp From River Waters In Iran. Parasitol Res 2015; 114(12): 4565-70. [DOI:10.1007/s00436-015-4702-x]
6. Noroozi Karbasdehi V, Dobaradaran S, Mirahmadi SR, et al. Survey of microbiological and chemical quality of the swimming beaches along the Persian Gulf in Bushehr port. Iran South Med J 2015; 18(2):393-408.
7. Kabil NS, Magdy R. Protective & Corrective Efficacy Of Xylitol Versus Fluoride On Primary Teeth Enamel Subjected To In Vitro Demineralization By Chlorinated Pool Water. Int J Dent Oral Heal 2016; 2(5): 66-73. [DOI:10.25141/2471-657X-2016-5.0108]
8. Hsu BM, Yeh HH. Removal Of Giardia And Cryptosporidium In Drinking Water Treatment: A Pilot-Scale Study. Water Res 2003; 37(5): 1111-7. [DOI:10.1016/S0043-1354(02)00466-9]
9. Rahdar M, Daylami O. The Comparison Of Chlorine And Ultra Violet Effects Against Giardia Lamblia Cyst In Drinking Water. Biochem Cell Arch 2016; 16(2): 369-72.
10. Dalimi A, Ghasemikhah R, Hashemi Malayeri B. Echinococcus Granulosus: Lethal Effect Of Low Voltage Direct Electric Current On Hydatid Cyst Protoscoleces. Exp Parasitol 2005; 109(4): 237-40. [DOI:10.1016/j.exppara.2004.12.014]
11. Khokhlova G, Vainshtein M. Application Of Static And Impulse Magnetic Fields To Bacteria Rhodospirillum Rubrum VKM B-1621. AMB Express 2017; 7(1): 60. [DOI:10.1186/s13568-017-0362-9]
12. Salmen SH, Alharbi SA, Faden AA, et al. Evaluation Of Effect Of High Frequency Electromagnetic Field On Growth And Antibiotic Sensitivity Of Bacteria. Saudi J Biol Sci 2018; 25(1): 105-10. [DOI:10.1016/j.sjbs.2017.07.006]
13. Bayır E, Bilgi E, Şendemir-Ürkmez A, et al. The Effects Of Different Intensities, Frequencies And Exposure Times Of Extremely Low-Frequency Electromagnetic Fields On The Growth Of Staphylococcus Aureus And Escherichia Coli O157: H7. Electromagn Biol Med 2015; 34(1): 14-8. [DOI:10.3109/15368378.2013.853671]
14. Oncul S, Cuce EM, Aksu B, et al. Effect Of Extremely Low Frequency Electromagnetic Fields On Bacterial Membrane. Int J Radiat Biol 2016; 92(1): 42-9. [DOI:10.3109/09553002.2015.1101500]
15. Boland-Nazar NS, Eslamirad Z, Sarmadian H, et al. An In Vitro Evaluation Of Ozonized Organic Extra-Virgin Olive Oil On Giardia Lamblia Cysts. Jundishapur J Microbiol 2016; 9(11): E40839. [DOI:10.5812/jjm.40839]
16. Moutinho MG, Dos Santos I, Matos M, et al. Research And Identification Of Cryptosporidium And Giardia Lamblia In Wastewater Treatment Plants. Ann Med 2019; 51(Sup1): 73. [DOI:10.1080/07853890.2018.1561629]
17. Efstratiou A, Ongerth J, Karanis P. Evolution Of Monitoring For Giardia And Cryptosporidium In Water. Water Res 2017; 123: 96-112. [DOI:10.1016/j.watres.2017.06.042]
18. Keserue HA, Füchslin HP, Egli T. Rapid Detection And Enumeration Of Giardia Lamblia Cysts In Water Samples By Immunomagnetic Separation And Flow Cytometric Analysis. Appl Environ Microbiol 2011; 77(15): 5420-7. [DOI:10.1128/AEM.00416-11]
19. Çulha G, Sangün MK. Serum Levels Of Zinc, Copper, Iron, Cobalt, Magnesium, And Selenium Elements In Children Diagnosed With Giardia Intestinalis And Enterobiosis Vermicularis In Hatay, Turkey. Biol Trace Elem Res 2007; 118(1): 21-6. [DOI:10.1007/s12011-007-0017-9]
20. Zhang L, Ji X, Yang X, et al. Cell Type-And Density-Dependent Effect Of 1 T Static Magnetic Field On Cell Proliferation. Oncotarget 2017; 8(8): 13126. [DOI:10.18632/oncotarget.14480]
21. Letuta UG, Berdinskiy VL. Magnetosensitivity Of Bacteria E. Coli: Magnetic Isotope And Magnetic Field Effects. Bioelectromagnetics 2017; 38(8): 581-91. [DOI:10.1002/bem.22073]
22. Zhao J, Li YG, Deng KQ, et al. Therapeutic Effects Of Static Magnetic Field On Wound Healing In Diabetic Rats. J Diabetes Res 2017; 2017: 6305370. [DOI:10.1155/2017/6305370]
23. Sztafrowski D, Suchodolski J, Muraszko J, et al. The Influence Of N And S Poles Of Static Magnetic Field (SMF) On Candida Albicans Hyphal Formation And Antifungal Activity Of Amphotericin B. Folia Microbiol 2019; 64(6): 727-34. [DOI:10.1007/s12223-019-00686-3]
24. Gilson RC, Deissler RJ, Bihary RF, et al. Growth Of Plasmodium Falciparum In Response To A Rotating Magnetic Field. Malaria J 2018; 17(1): 190. [DOI:10.1186/s12936-018-2333-2]
25. Ramadan Q, Christophe L, Teo W, et al. Flow-Through Immunomagnetic Separation System For Waterborne Pathogen Isolation And Detection: Application To Giardia And Cryptosporidium Cell Isolation. Anal Chim Acta 2010; 673(1): 101-8. [DOI:10.1016/j.aca.2010.05.025]
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Ghasemikhah R, Davudi Moghadam Z, Ahmadi F, Rasulpur M, Bayatiani M R, Hajihossein R, et al . Magnetic Separation of Giardia Cysts from Water. Iran South Med J. 2020; 23 (2) :99-107
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Volume 23, Issue 2 (Iranian South Medical Journal 2020) Back to browse issues page
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