Volume 23, Issue 2 (Iranian South Medical Journal 2020)
Iran South Med J 2020, 23(2): 99-107 |
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Ghasemikhah R, Davudi Moghadam Z, Ahmadi F, Rasulpur M, Bayatiani M R, Hajihossein R, et al . Magnetic Separation of Giardia Cysts from Water. Iran South Med J 2020; 23 (2) :99-107
URL: http://ismj.bpums.ac.ir/article-1-1291-en.html
URL: http://ismj.bpums.ac.ir/article-1-1291-en.html
Reza Ghasemikhah1 
, Zainab Davudi Moghadam2 , Fatemeh Ahmadi2 , Mahsa Rasulpur2 , Mohammad Reza Bayatiani3 , Reza Hajihossein1 , Hossein Sarmadian4 , Fatemeh Seif * 5
, Zainab Davudi Moghadam2 , Fatemeh Ahmadi2 , Mahsa Rasulpur2 , Mohammad Reza Bayatiani3 , Reza Hajihossein1 , Hossein Sarmadian4 , Fatemeh Seif * 5
1- Department of Parasitology and Mycology, School of Medicine, Arak University of Medical Sciences, Arak, Iran
2- Students Research Committee, School of Paramedical Sciences, Arak University of Medical Sciences, Arak, Iran
3- Department of Radiotherapy and Medical Physics, School of Paramedical Sciences, Arak University of Medical sciences and Khansari Hospital, Arak, Iran
4- Department of Infectious Disease, School of Medicine, Arak University of Medical Sciences, Arak, Iran
5- Department of Radiotherapy and Medical Physics, School of Paramedical Sciences, Arak University of Medical sciences and Khansari Hospital, Arak, Iran ,seif@arakmu.ac.ir
2- Students Research Committee, School of Paramedical Sciences, Arak University of Medical Sciences, Arak, Iran
3- Department of Radiotherapy and Medical Physics, School of Paramedical Sciences, Arak University of Medical sciences and Khansari Hospital, Arak, Iran
4- Department of Infectious Disease, School of Medicine, Arak University of Medical Sciences, Arak, Iran
5- Department of Radiotherapy and Medical Physics, School of Paramedical Sciences, Arak University of Medical sciences and Khansari Hospital, Arak, Iran ,
Abstract: (2801 Views)
Background: Giardia is one of the infectious agents that can be transmitted by surface water and cause a disease. We designed and fabricated a magnetic system to separate the giardia cysts from water.
Materials and Methods: In this study, giardia cysts were concentrated by sucrose floatation method. The cysts were mixed with physiological saline and their concentration was adjusted to 100,000 cysts per cubic millimeter using Neubauer slide. The isolated cysts were passed through the magnetic field generated by the 200-msT magnets. The cysts were attracted to the magnet and removed from the suspension. The cysts were exposed to magnetic field for one week in order to evaluate the effect of magnetic field on their survival, which was reported as 0.1% using eosin staining under a light microscope.
Results: The results showed that magnetic field can relatively absorb giardia cysts from water, the survival rate of the cysts did not change significantly in different days (P≥0.05).
Conclusion: The designed magnetic system can be reinforced to isolate giardia cysts, which is economical and has no chemical effects on water.
Materials and Methods: In this study, giardia cysts were concentrated by sucrose floatation method. The cysts were mixed with physiological saline and their concentration was adjusted to 100,000 cysts per cubic millimeter using Neubauer slide. The isolated cysts were passed through the magnetic field generated by the 200-msT magnets. The cysts were attracted to the magnet and removed from the suspension. The cysts were exposed to magnetic field for one week in order to evaluate the effect of magnetic field on their survival, which was reported as 0.1% using eosin staining under a light microscope.
Results: The results showed that magnetic field can relatively absorb giardia cysts from water, the survival rate of the cysts did not change significantly in different days (P≥0.05).
Conclusion: The designed magnetic system can be reinforced to isolate giardia cysts, which is economical and has no chemical effects on water.
Type of Study: Original |
Subject:
Parasitology
Received: 2019/10/28 | Accepted: 2020/02/5 | Published: 2020/06/27
Received: 2019/10/28 | Accepted: 2020/02/5 | Published: 2020/06/27
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