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:: Volume 17, Issue 2 (Iranian South Medical journal 2014) ::
Iran South Med J 2014, 17(2): 150-160 Back to browse issues page
Expression profile of germ stem cell-specific genes in human spermatogonial stem cells after co culture with sertoli cells
Maria Zahiri1, Mansooreh Movahedin 2, Seyyed Javad Mowla3, Mehrdad Noruzinia4, Mohammad Reza Noroozi5, Naser Amirjanati6
1- Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IRAN
Department of Anatomical Sciences, Faculty of Medical Sciences, Bushehr University of Medical Sciences, Bushehr, IRAN
2- Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IRAN , m.movahed@modares.ac.ir
3- Department of Genetics, Faculty of Basic Sciences, Tarbiat Modares University, Tehran, IRAN
4- Department of Medical genetics, Faculty of Medecine, Tarbiat Modares University, Tehran, IRAN
5- Uro -Oncology Research Center, Tehran University of Medical Sciences, Tehran, IRAN
6- Reproductive Biotechnology Research Center, Avicenna Research Institute (ACECR), Tehran, IRAN
Abstract:   (6483 Views)

Background: Human spermatogonial stem cells (SSCs), are the foundation of spermatogenesis. Because of low number and lack of significant marker in human SSCs, studying their characteristics, could provide better understanding about the biology of male fertility. This study was designed to examine the effects of in vitro co-culture with sertoli cells on SSC colonization and germ cells specific gene expression of human spermatogonial stem cells. Material and Methods: Testicular cells were isolated from testis biopsies by using two step enzymatic digestion and differential plating. two culture system were designed: co-culture with patient Sertoli cells and culture of SSC without co-culture(as control group). The number and diameter of colonies were evaluated during 3 weeks of culture. The expression of alpha 6 integrin, beta1 integrin and PLZF, as germ stem cell specific markers, was assessed using quantitative RT-PCR. Statistical analysis was performed using one way ANOVA in SPSS vesion 16 software with 95% Confidence interval . Result: Our results were showed that the number and diameter of colonies increased significantly in co-culture with sertoli cells (P<0.05). The expression profile of genes in 2nd and 3rd weeks of culture revealed that there is significant higher expression of germ stem cell markers in our co-culture group versus control group. Conclusion: Based on the optimal effects of sertoli cells on spermatogonial stem cells, co culture of the human SSCs with the feeder layer sertoli may be used as a suitable method for the enrichment of human spermatogonial stem cells.

Keywords: human Spermatogonial Stem Cells, Colony Formation, Culture, gene expression
Full-Text [PDF 964 kb]   (1997 Downloads)    
Type of Study: Original | Subject: General
Received: 2012/06/13 | Accepted: 2012/10/10 | Published: 2014/04/14
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Zahiri M, Movahedin M, Mowla S J, Noruzinia M, Noroozi M R, Amirjanati N. Expression profile of germ stem cell-specific genes in human spermatogonial stem cells after co culture with sertoli cells. Iran South Med J. 2014; 17 (2) :150-160
URL: http://ismj.bpums.ac.ir/article-1-521-en.html


Volume 17, Issue 2 (Iranian South Medical journal 2014) Back to browse issues page
دانشگاه علوم پزشکی بوشهر، طب جنوب ISMJ

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