[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 23, Issue 5 (Iranian South Medical Journal 2020) ::
Iran South Med J 2020, 23(5): 431-441 Back to browse issues page
Effects of Nano-Chitosan Loaded with Donepezil on Ethanol Induced Amnesia in Swiss-type Mice
Mahmood Heidari1 , Nasrin Sadat Azami 2, Mehdi Ebadi3
1- Department of Biology, School of Science, Islamic Azad University, Gorgan Branch, Gorgan, Iran
2- Department of Biology, School of Science, Islamic Azad University, Gorgan Branch, Gorgan, Iran , Azaminasrin@gmail.com
3- Department of Chemistry, School of Science, Islamic Azad University, Gorgan Branch, Gorgan, Iran
Abstract:   (192 Views)
Background: Progressive neurodegeneration of nervous system as well as learning and memory disorders is characteristics of Alzheimer’s disease. Drug delivery is one of the research aspects for improving treatment of diseases. Chitosan nanoparticles were used for Donepezil transportation and its effect on
dimproving amnesia was investigated.
Materials and Methods: In this experimental study, 136 NMRI mice weighing 30-40 mg were used. All injections were done intraperitoneally. The first experiment aimed to determine the optimal dose of ethanol for inducing amnesia, the second experiment was performed to prove nano-chitosan loaded with Donepezil (0.25, 0.5, 0.75, 1 mg/kg) does not impair memory, and the third experiment assessed the effect of nano-chitosan loaded with different doses of Donepezil on amnesia induced by ethanol. Data were recorded as mean ± SD, analyzed in GraphPad Prism software using ANOVA and Tukey post hoc test. P<0.05 was considered as significant.    
Results: The first experiment showed that ethanol (0.5, 0.75, 1 mg/kg) could significantly induce amnesia. The second experiment showed nano-chitosan loaded with Donepezil doid not impair memory. The third experiment showed that all nano-chitosan groups loaded with different doses of Donepezil could significantly improve amnesia induced by ethanol.
Conclusion: Chitosan nanoparticles used in this study can efficiently be used for drug delivery. However, further research should be done on this compound before it can be used for oral drug transportation.
Keywords: Amnesia, Chitosan, Donepezil, Ethanol, Nanoparticle
Full-Text [PDF 445 kb]   (63 Downloads)    
Type of Study: Original | Subject: Physiology
Received: 2020/03/2 | Accepted: 2020/08/2 | Published: 2020/11/7
References
1. Eskandary A, Moazedi AA, Najaph Zade H, et al. Research Paper: Effects Of Donepezil Hydrochloride On Neuronal Response Of Pyramidal Neurons Of The CA1 Hippocampus In Rat Model Of Alzheimer’s Disease. Basic Clin Neurosci 2019; 10(2): 109-17.
2. Amani M. Pathophysiology Of Alzheimer’s Disease. J Ardabil Univ Med Sci 2017; 16(4): 452-63. (Persian)
3. Palop JJ, Mucke L. Amyloid-Β Induced Neuronal Dysfunction In Alzheimer’s Disease: From Synapses Toward Neural Networks. Nat Neurosci 2010; 13(7): 812-8.
4. Zali H, Sadat Seyyedi S, Rashidy Pour A, et al. Epidemiology And Etiology Of Alzheimer’s Disease. Koomesh 2015; 16(2): 119-27. (Persian)
5. Congdon EE, Sigurdsson EM. Tau-targeting therapies for Alzheimer disease. Nat Rev Neurol 2019; 14(7): 399-415.
6. Black SE, Doody R, Li H, et al. Donepezil Preserves Cognition And Global Function In Patients With Severe Alzheimer Disease. Neurology 2007; 69(5): 459-69.
7. Kim SH, Kandiah N, Hsu JL, et al. Beyond Symptomatic Effects: Potential Of Donepezil As A Neuroprotective Agent And Disease Modifier In Alzheimer’s Disease. Br J Pharmacol 2017; 174(23): 4224-32.
8. Di Stefano A, Iannitelli A, Laserra S, et al. Drug Delivery Strategies For Alzheimer’s Disease Treatment. Expert Opin Drug Deliv 2011; 8(5): 581-603.
9. Kogan MJ, Bastus NG, Amigo R, et al. Nanoparticle-Mediated Local And Remote Manipulation Of Protein Aggregation. Nano Lett 2006; 6(1): 110-5.
10. Raafat D, Sahl HG. Minireview Chitosan And Its Antimicrobial Potential -- A Critical Literature Survey. Microb Biotechnol 2009; 2(2): 186-201.
11. Tang C, Chen N, Zhang Q, et al. Preparation And Properties Of Chitosan Nanocomposites With Nanofillers Of Different Dimensions. Polym Degrad Stabil 2009; 94(1): 124-31.
12. Azari A, Rezaei Kalantary R, Keramaty A, et al. Extraction and optimization of chitosan from shrimp shell: preparation, characterization and application in fluoride removal (isotherm, kinetic and thermodynamic studies). Iran South Med J 2016; 19(4): 644-61. (Persian)
13. Anjireddy K, Karpagam S. Chitosan Nanofilm And Electrospun Nanofiber For Quick Drug Release In The Treatment Of Alzheimer’s Disease: In Vitro And In Vivo Evaluation. Int J Biol Macromol 2017; 105(Pt 1): 131-42.
14. Sadeghi M, Ganji F, Taghizadeh SM, et al. Preparation And Characterization Of Rivastigmine Transdermal Patch Based On Chitosan Microparticles. Iran J Pharm Res 2016; 15(3): 283-94.
15. Mendes AC, Gorzelanny C, Halter N, et al. Hybrid Electrospun Chitosan-Phospholipids Nanofibers For Transdermal Drug Delivery. Int J Pharm 2016; 510(1): 48-56.
16. Hamidi M, Azadi A, Rafiei P. Hydrogel Nanoparticles In Drug Delivery. Adv Drug Deliv Rev 2008; 60(15): 1638-49.
17. Seifhosseini S, Jahanshahi M, Moghimi A, et al. The Effect Of Scopolamine On Avoidance Memory And Hippocampal Neurons In Male Wistar Rats. Basic Clin Neurosci 2011; 3(1): 9-15.
18. Feizolahi F, Azarbayjani MA, Nasehi M, et al. Comparison The Effect Of Short-Term Swimming Training And Curcumin Supplementation On Damaged Spatial Memory After Binge Ethanol Drinking In Male Rats: Preliminary Report. J Med Plants 2017; 16(61 And S10 ): 174-84. (Persian)
19. Zavvari F, Karimzadeh F. A Review On The Behavioral Tests For Learning And Memory Assessments In Rat. Neurosci J Shefaye Khatam 2017; 5(4): 110-24. (Persian)
20. Piri M, Moshfegh A, Raoufi N, et al. The Influence Of L-Arginine In The Improving Effect Of Nicotine On Ethanol- Induced Amnesia. J Anim Biol 2012; 4(2): 23-32. (Persian)
21. Pakpour B, Hadidi AH, Navaeian M, et al. Repair Ethanol Induced Amnesia With Iranian Lactobacillus Brevis In Rats. J Appl Microbiol Food Ind 2015; 1(1): 1-11. (Persian)
22. Hajizadeh E, Khezri S, Piri M. Influence Of Ethanol On Amnesia And State-Dependent Learning Induced By Scopolamine In Male Mouse. J Anim Biol 2014; 6(4): 1-11. (Persian)
23. Asadi Motlagh M, Pakpour B, Navaian M. The Effect Of 5-HT3 Receptor Agonist Of The Ventral Hippocampus On Amnesia Induced By Ethanol In Mice. Feyz J Kashan Univ Med Sci 2018; 22(3): 239-47. (Persian)
24. Rajaei F, Moghaddam-Shad M, Salehi Z. The Effects Of Amygdalin On The Morphologic Structure Of Mouse Cerebellar Cortex. J Shahrekord Univ Med Sci 2013; 15(3): 26-32. (Persian)
25. Balsters JH, O’Connell RG, Martin MP, et al. Donepezil Impairs Memory In Healthy Older Subjects: Behavioural, EEG And Simultaneous EEG/fMRI Biomarkers. Plos One 2011; 6(9): e24126.
26. Repantis D, Laisney O, Heuser I. Acetylcholinesterase Inhibitors And Memantine For Neuroenhancement In Healthy Individuals: A Systematic Review. Pharmacol Res 2010; 61(6): 473-81.
27. Al Asmari AK, Ullah Z, Tariq M, et al. Preparation, Characterization, And In Vivo Evaluation Of Intranasally Administered Liposomal Formulation Of Donepezil. Drug Des Devel Ther 2016; 10: 205-15.
28. Zhang P, Chen L, Gu W, et al. In Vitro And In Vivo Evaluation Of Donepezil-Sustained Release Microparticles For The Treatment Of Alzheimer’s Disease. Biomaterials 2007; 28(10): 1882-8.
29. Abruzzo A, Bigucci F, Cerchiara T, et al. Mucoadhesive Chitosan/Gelatin Films For Buccal Delivery Of Propranolol Hydrochloride. Carbohyd Polym 2012; 87(1): 581-8.
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Heidari M, Azami N S, Ebadi M. Effects of Nano-Chitosan Loaded with Donepezil on Ethanol Induced Amnesia in Swiss-type Mice. Iran South Med J. 2020; 23 (5) :431-441
URL: http://ismj.bpums.ac.ir/article-1-1349-en.html


Volume 23, Issue 5 (Iranian South Medical Journal 2020) Back to browse issues page
دانشگاه علوم پزشکی بوشهر، طب جنوب ISMJ

Iranian South Medical Journal is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License which allows users to read,
copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly

Copyright © 2017, Iranian South Medical Journal| All Rights Reserved

Persian site map - English site map - Created in 0.04 seconds with 30 queries by YEKTAWEB 4256