[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 23, Issue 5 (Iranian South Medical Journal 2020) ::
Iran South Med J 2020, 23(5): 505-514 Back to browse issues page
The Effects of Thymoquinone on Semen Quality in the Diazinon Exposed Rats
Lila Tajik1 , Sima Nasri 2, Ehsan Raeis-Abdollahi3
1- Department of Biology, Payame Noor University, Tehran, Iran
2- Department of Biology, Payame Noor University, Tehran, Iran , s_nasri1@pnu.ac.ir
3- Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Abstract:   (245 Views)
Background: Exposure to organophosphate pesticides, including diazinon, can lead to increased production of free radicals and decreased cellular antioxidant capacity and male infertility. Thymoquinone (TQ) is an active component of Nigella sativa with well documented antioxidant effects. This study evaluated the effects of thymoquinone on oxidative stress induced by exposure to diazinon and semen quality in male rats.
Materials and Methods: Twenty-eight male Wistar rats weighing 200-300 g were randomly divided into four groups (n=7, each). The first (control) group received a single intraperitoneal (i.p.) injection of olive oil (medication vehicle). Testicular toxicity was induced in rats of the second and third groups by a single injection of diazinon (20 mg/kg, i.p.). Also, the animals of the second and third groups received the vehicle of TQ (olive oil) or TQ (10 mg/kg/day, i.p.), respectively, for five consecutive days starting 3 days before diazinon administration. The fourth group of animals received thymoquinone (10 mg/kg/day) for five consecutive days without induction of diazinon toxicity. Thirty-six hours later, semen quality was assessed for motility, morphology, and viability of sperms in addition to measurement of malondialdehyde (MDA) level in testis tissue.
Results: The results showed that the sperm motility significantly decreased and MDA level significantly increased (P<0.0001) after diazinon exposure whereas thymoquinone increased sperm motility and decreased MDA level (P<0.0001) in testis tissue after exposure to diazinon as compared to the control group.
Conclusion: Since diazinon increases lipid peroxidation in sperm plasma membrane through production of free radicals, it can be concluded that administration of thymoquinone as an antioxidant can prevent diazinon toxicity in testis tissue of rats, and improve the quality of semen.
 
Keywords: Semen quality, Thymoquinone, Diazinon, Lipid peroxidation, Male Rat
Full-Text [PDF 402 kb]   (45 Downloads)    
Type of Study: Original | Subject: Physiology
Received: 2019/08/13 | Accepted: 2019/12/31 | Published: 2020/11/7
References
1. Pina-Guzman B, Solis-Heredia MJ, Quintanilla-Vega B. Diazinon Alters Sperm Chromatin Structure In Mice By Phosphorylating Nuclear Protamines. Toxicol Appl Pharmacol 2005; 202(2): 189-98.
2. Rahimi Anbarkeh F, Nikravesh MR, Jalali M, et al. Single Dose Effect Of Diazinon On Biochemical Parameters In Testis Tissue Of Adult Rats And The Protective Effect Of Vitamin E. Iran J Reprod Med 2014; 12(11): 731-6.
3. Oruc E. Effects Of Diazinon On Antioxidant Defense System And Lipid Peroxidation In The Liver Of Cyprinus Carpio (L.). Environ Toxicol 2011; 26(6): 571-8.
4. Shah MD, Iqbal M. Diazinon-Induced Oxidative Stress And Renal Dysfunction In Rats. Food Chem Toxicol 2010; 48(12): 3345-53.
5. Perez-Herrera N, Polanco-Minaya H, Salazar-Arredondo E, et al. PON1Q192R Genetic Polymorphism Modifies Organophosphorous Pesticide Effects On Semen Quality And DNA Integrity In Agricultural Workers From Southern Mexico. Toxicol Appl Pharmacol 2008; 230(2): 261-8.
6. Swan SH, Kruse RL, Liu F, et al. Semen Quality In Relation To Biomarkers Of Pesticide Exposure. Environ Health Perspect 2003; 111(12): 1478-84.
7. Darakhshan S, Bidmeshki Pour A, Hosseinzadeh Colagar A, et al. Thymoquinone And Its Therapeutic Potentials. Pharmacol Res 2015; 95-96: 138-58.
8. Goyal SN, Prajapati CP, Gore PR, et al. Therapeutic Potential And Pharmaceutical Development Of Thymoquinone: A Multitargeted Molecule Of Natural Origin. Front Pharmacol 2017; 8: 656.
9. Halliwell B. Free Radicals And Antioxidants: Updating A Personal View. Nutr Rev 2012; 70(5): 257-65.
10. Tomokuni K, Hasegawa T, Hirai Y, et al. The Tissue Distribution Of Diazinon And The Inhibition Of Blood Cholinesterase Activities In Rats And Mice Receiving A Single Intraperitoneal Dose Of Diazinon. Toxicology 1985; 37(1-2): 91-8.
11. Fouad AA, Jresat I. Thymoquinone Therapy Abrogates Toxic Effect Of Cadmium On Rat Testes. Andrologia 2015; 47(4): 417-26.
12. Toman R, Hluchy S, Cabaj M, et al. Effect Of Separate And Combined Exposure Of Selenium And Diazinon On Rat Sperm Motility By Computer Assisted Semen Analysis. J Trace Elem Med Biol 2016; 38: 144-9.
13. Ola-Mudathir KF, Suru SM, Fafunso MA, et al. Protective Roles Of Onion And Garlic Extracts On Cadmium-Induced Changes In Sperm Characteristics And Testicular Oxidative Damage In Rats. Food Chem Toxicol 2008; 46(12): 3604-11.
14. Cooper TG, Noonan E, Von Eckardstein S, et al. World Health Organization Reference Values For Human Semen Characteristics. Hum Reprod Update 2010; 16(3): 231-45.
15. Noroozzadeh M, Ramezani Tehrani F, ZadehVakili A, et al. The effects of testosterone intrauterine disturbance on sperm quality and testis tissue in male rat,s offspring after puberty. Iran South Med J 2016; 19(3): 372-84.
16. Halvaei I, Roodsari HS, Harat ZN. Acute Effects Of Ruta Graveolens L. On Sperm Parameters And DNA Integrity In Rats. J Reprod Infertil 2012; 13(1): 33-8.
17. Esterbauer H, Cheeseman KH. Determination Of Aldehydic Lipid Peroxidation Products: Malonaldehyde And 4-Hydroxynonenal. Methods Enzymol 1990; 186: 407-21.
18. Bisht S, Faiq M, Tolahunase M, et al. Oxidative Stress And Male Infertility. Nat Rev Urol 2017; 14(8): 470-85.
19. Greil AL, Slauson-Blevins K, Mcquillan J. The Experience Of Infertility: A Review Of Recent Literature. Sociol Health Illn 2010; 32(1): 140-62.
20. Tvrda E, Knazicka Z, Bardos L, et al. Impact Of Oxidative Stress On Male Fertility - A Review. Acta Vet Hung 2011; 59(4): 465-84.
21. Hikim AP, Maiti BR, Ghosh A. Spermatogenesis In The Bandicoot Rat. I. Duration Of The Cycle Of The Seminiferous Epithelium. Arch Androl 1985; 14(2-3): 151-4.
22. Salahshoor MR, Haghjoo M, Jalali C, et al. Effect Of Thymoquinone On Reproductive Parameter In Morphine-Treated Male Mice. Adv Biomed Res 2018; 7: 18.
23. Agarwal A, Roychoudhury S, Bjugstad KB, et al. Oxidation-Reduction Potential Of Semen: What Is Its Role In The Treatment Of Male Infertility?. Ther Adv Urol 2016; 8(5): 302-18.
24. Majzoub A, Agarwal A. Antioxidant Therapy In Idiopathic Oligoasthenoteratozoospermia. Indian J Urol 2017; 33(3): 207-14.
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Tajik L, Nasri S, Raeis-Abdollahi E. The Effects of Thymoquinone on Semen Quality in the Diazinon Exposed Rats. Iran South Med J. 2020; 23 (5) :505-514
URL: http://ismj.bpums.ac.ir/article-1-1355-en.html


Volume 23, Issue 5 (Iranian South Medical Journal 2020) Back to browse issues page
دانشگاه علوم پزشکی بوشهر، طب جنوب ISMJ

Iranian South Medical Journal is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License which allows users to read,
copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly

Copyright © 2017, Iranian South Medical Journal| All Rights Reserved

Persian site map - English site map - Created in 0.05 seconds with 30 queries by YEKTAWEB 4256