Volume 25, Issue 1 (Iranian South Medical Journal 2022)
Iran South Med J 2022, 25(1): 1-12 |
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Ahmadi M, Eidi A, Ahmadvand H, Khaksarian M, Sotoodehnejadnematalahi F. The Effect of Carvacrol on the Expression of Genes Hmox-1, iNOS, Nrf2 and NF-ҚB in the Spinal Cord of Experimental Autoimmune Encephalomyelitis Mice. Iran South Med J 2022; 25 (1) :1-12
URL: http://ismj.bpums.ac.ir/article-1-1544-en.html
URL: http://ismj.bpums.ac.ir/article-1-1544-en.html
Mahdeiah Ahmadi1 
, Akram Eidi1 , Hassan Ahmadvand * 2, Mojtaba Khaksarian3 , Fattah Sotoodehnejadnematalahi1
, Akram Eidi1 , Hassan Ahmadvand * 2, Mojtaba Khaksarian3 , Fattah Sotoodehnejadnematalahi1
1- Department of Biology, School of Convergent Sciences and Technologies, Islamic Azad University, Science and Research Branch, Tehran, Iran
2- Department of Biochemistry, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran ,Hassan_a46@yahoo.com
3- Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
2- Department of Biochemistry, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran ,
3- Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
Abstract: (2577 Views)
Background: Multiple sclerosis (MS) is one of the most common diseases of the nervous system, characterized by inflammation of the central nervous system and oxidative stress. Carvacrol is a monoterpenoid phenol with antioxidant effects against free radical. The aim of this study was to evaluate the effect of carvacrol on the expression of Hmox-1, iNOS, Nrf2 and NF-ҚB genes in the spinal cord of Experimental Autoimmune Encephalomyelitis (EAE) rats as an animal model of MS.
Materials and Methods: EAE was induced in female Lewis rats and they were then divided into three groups: control, EAE model, and EAE treated with carvacrol. Carvacrol was daily injected intraperitoneally for 17 days after immunization. RNA was extracted from rat spinal cord tissue and changes in the expression of the Hmox-1, iNOS, Nrf2 and NF-ҚB genes were examined by Real-Time PCR.
Results: The results showed that carvacrol treatment stopped severe weight loss in the EAE model group and their mean weight increased. Furthermore, carvacrol was found to reduce the expression of iNOS and NF-ҚB genes and increase the levels of Hmox-1 mRNA and Nrf2 mRNA.
Conclusion: The effect of carvacrol on weight changes in treated rats, along with the increased HO-1 gene expression in their spinal cord, associated with increased Nrf2 mRNA and decreased expression of iNOS and NF-ҚB, led to a reduction in inflammation and oxidative stress, and an increase in the neuroprotection. Furthermore, the invasive environment created for some cells, such as oligodendrocytes, was modulated.
Type of Study: Original |
Subject:
Physiology
Received: 2021/09/24 | Accepted: 2022/01/15 | Published: 2022/03/13
Received: 2021/09/24 | Accepted: 2022/01/15 | Published: 2022/03/13
References
1. Kasper LH, Shoemaker J. Multiple Sclerosis Immunology: The Healthy Immune System vs The MS Immune System. Neurology 2010; 74(Suppl 1): S2-S8. [DOI]
2. Kim DY, Hao J, Liu R, et al. Inflammation-Mediated Memory Dysfunction And Effects Of A Ketogenic Diet In A Murine Model Of Multiple Sclerosis. PLoS One 2012; 7(5): e35476. [DOI]
3. Gray E, Thomas TL, Betmouni S, et al. Elevated Myeloperoxidase Activity In White Matter In Multiple Sclerosis. Neurosci Lett 2008; 444(2): 195-8. [DOI]
4. Valko M, Leibfritz D, Moncol J, et al. Free Radicals And Antioxidants In Normal Physiological Functions And Human Disease. Int J Biochem Cell Biol 2007; 39(1): 44-84. [DOI]
5. Coyle JT, Puttfarcken P. Oxidative Stress, Glutamate, And Neurodegenerative Disorders. Science 1993; 262(5134): 689-95. [DOI]
6. Khazdair MR, Boskabady MH. The Effect Of Carvacrol On Inflammatory Mediators And Respiratory Symptoms In Veterans Exposed To Sulfur Mustard, A Randomized, Placebo-Controlled Trial. Respir Med 2019; 150: 21-9. [DOI]
7. Baluchnejadmojarad T, Hassanshahi J, Roghani M, et al. Protective Effect Of Carvacrol In 6-Hydroxydopamine Hemi-Parkinsonian Rat Model. J Basic Clin Pathophysiol 2014; 2(2): 29-34. [Article] [Google Scholar]
8. Baluchnejadmojarad T, Roghani M. The Protective Effect Of Carvacrol On Kainic Acid-Induced Model Of Temporal Lobe Epilepsy In Male Rat. J Basic Clin Pathophysiol 2016; 4(2): 11-6. [Article] [DOI]
9. Gholijani N, Amirghofran Z. Effects Of Thymol And Carvacrol On T-Helper Cell Subset Cytokines And Their Main Transcription Factors In Ovalbumin-Immunized Mice. J Immunotoxicol 2016; 13(5): 729-37. [DOI]
10. Zhou Y, Cui C, Ma X, et al. Nuclear Factor κB (NF-κB)–Mediated Inflammation in Multiple Sclerosis. Front Immunol 2020; 11: 391. [DOI]
11. Liu Y, Zhu B, Luo L, et al. Heme Oxygenase-1 Plays An Important Protective Role In Experimental Autoimmune Encephalomyelitis. Neuroreport 2001; 12(9): 1841-5. [DOI]
12. Lan M, Tang X, Zhang J, et al. Insights In Pathogenesis Of Multiple Sclerosis: Nitric Oxide May Induce Mitochondrial Dysfunction Of Oligodendrocytes. Rev Neurosci 2017; 29(1): 39-53. [DOI]
13. Johnson DA, Amirahmadi S, Ward C, et al. The Absence Of The Pro-Antioxidant Transcription Factor Nrf2 Exacerbates Experimental Autoimmune Encephalomyelitis. Toxicol Sci 2010; 114(2): 237-46. [DOI]
14. Cerghet M, Skoff RP, Bessert D, et al. Proliferation And Death Of Oligodendrocytes And Myelin Proteins Are Differentially Regulated In Male And Female Rodents. J Neurosci 2006; 26(5): 1439-47. [DOI]
15. Yoshida H, Kimura A, Fukaya T, et al. Low Dose CP-690,550 (Tofacitinib), a pan-JAK Inhibitor, Accelerates The Onset Of Experimental Autoimmune Encephalomyelitis By Potentiating Th17 Differentiation. Biochem Biophys Res Commun 2012; 418(2): 234-40. [DOI]
16. Beeton C, Garcia A, Chandy KG. Induction And Clinical Scoring Of Chronic-Relapsing Experimental Autoimmune Encephalomyelitis. J Vis Exp 2007; (5): e224. [DOI]
17. Stosic‐Grujicic S, Ramic Z, Bumbasirevic Z, et al. Induction Of Experimental Autoimmune Encephalomyelitis In Dark Agouti Rats Without Adjuvant. Clin Exp Immunol 2004; 136(1): 49-55. [DOI]
18. Hofstetter HH, Shive CL, Forsthuber TG. Pertussis Toxin Modulates the Immune Response to Neuroantigens Injected in Incomplete Freund’s Adjuvant: Induction of Th1 Cells and Experimental Autoimmune Encephalomyelitis in the Presence of High Frequencies of Th2 Cells. J Immunol 2002; 169(1): 117-25. [DOI]
19. Mohajeri M, Sadeghizadeh M, Najafi F, et al. Polymerized Nano-Curcumin Attenuates Neurological Symptoms In EAE Model Of Multiple Sclerosis Through Down Regulation Of Inflammatory And Oxidative Processes And Enhancing Neuroprotection And Myelin Repair. Neuropharmacology 2015; 99: 156-67. [DOI]
20. Gholami M, Rajaei Z, Malek M. Effects Of Carvacrol On Spatial Learning Performances, Hippocampal Interleukin-1ß Level And Oxidative Stress Markers In Lipopolysaccharide-Treated Rats. Physiol Pharmacol 2019; 23(4): 286-95. [Article]
21. Ahmadi M, Eidi A, Ahmadvand H, et al. Effect Of Carvacrol On The Expression of IL-10, FOX-P3, IL-4 and TGF-β Genes In The Spinal Cord Of Rats Model Of Multiple Sclerosis. Iran J Biol Sci 2020; 15(2): 53-61. (Persian) [Article]
22. Pareek TK, Belkadi A, Kesavapany S, et al. Triterpenoid Modulation Of IL-17 and Nrf-2 Expression Ameliorates Neuroinflammation And Promotes Remyelination In Autoimmune Encephalomyelitis. Sci Rep 2011; 1: 201. [DOI]
23. Ahmed SMU, Luo L, Namani A, et al. Nrf2 Signaling Pathway: Pivotal Roles In Inflammation. Biochim Biophys Acta Mol Basis Dis 2017; 1863(2): 585-97. [DOI]
24. Habtemariam S. The Nrf2/HO-1 Axis As Targets For Flavanones: Neuroprotection By Pinocembrin, Naringenin, And Eriodictyol. Oxid Med Cell Longev 2019; 2019: 4724920. [DOI]
25. Movahed A. Beneficial Effects Of Resveratrol, Present In Grapes In The Prevention And Treatment Of Heart Disease And Failure. Iran South Med J 2015; 18(1): 183-99. (Persian) [Article]
26. Nitti M, Piras S, Brondolo L, et al. Heme Oxygenase 1 In The Nervous System: Does It Favor Neuronal Cell Survival Or Induce Neurodegeneration?. Int J Mol Sci 2018; 19(8): 2260. [DOI]
27. Alvi AM, Al Kury LT, Alattar A, et al. Carveol Attenuates Seizure Severity and Neuroinflammation in Pentylenetetrazole-Kindled Epileptic Rats by Regulating the Nrf2 Signaling Pathway. Oxid Med Cell Longev 2021; 2021: 9966663. [DOI]
28. Yue Y, Stone S, Lin W. Role Of Nuclear Factor κB In Multiple Sclerosis And Experimental Autoimmune Encephalomyelitis. Neural Regen Res 2018; 13(9): 1507-15. [DOI]
29. Hill KE, Zollinger LV, Watt HE, et al. Inducible Nitric Oxide Synthase In Chronic Active Multiple Sclerosis Plaques: Distribution, Cellular Expression And Association With Myelin Damage. J Neuroimmunol 2004; 151(1- 2): 171-9. [DOI]
30. Mirzazadeh E, Khezri S, Abtahi Froushani SM. Effects of Quercetin on Improving the Damage Caused by Free Radicals in the Rat Models of Multiple Sclerosis. Iran South Med J 2019; 22(1): 1-15. (Persian) [Article]
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