Volume 28, Issue 6 (Iran South Med J 2026)
Iran South Med J 2026, 28(6): 950-962 |
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Ahmadi H, Akbari Z, Mohammadi M, Esmaeilzade B, Hemmatjou I, Zahiri M. The Effect of Alcoholic Extract of Aphani-zomenon Flos-aqua Algae on the Adhesion and Survival of Human Mesenchymal Stem Cells Cultured on Polycaprolactone/ Gelatin Composite Scaffold. Iran South Med J 2026; 28 (6) :950-962
URL: http://ismj.bpums.ac.ir/article-1-2477-en.html
URL: http://ismj.bpums.ac.ir/article-1-2477-en.html
Houssein Ahmadi1 
, Zahra Akbari2 , Mahdi Mohammadi3 , Banafshe Esmaeilzade1 , Iman Hemmatjou4 , Maria Zahiri *5
, Zahra Akbari2 , Mahdi Mohammadi3 , Banafshe Esmaeilzade1 , Iman Hemmatjou4 , Maria Zahiri *5
1- Department of Anatomical Sciences, School of Medical Sciences, Bushehr University of Medical Sciences, Bushehr, Iran
2- Department of Physiology, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
3- School of Persian Gulf Research Institute, Department of Biotechnology, University of Persian Gulf, Bushehr, Iran
4- Student Research Committee, Bushehr University of Medical Sciences, Bushehr, Iran
5- Department of Anatomical Sciences, School of Medical Sciences, Bushehr University of Medical Sciences, Bushehr, Iran
Student Research Committee, Bushehr University of Medical Sciences, Bushehr, Iran ,m.zahiri@bpums.ac.ir
2- Department of Physiology, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
3- School of Persian Gulf Research Institute, Department of Biotechnology, University of Persian Gulf, Bushehr, Iran
4- Student Research Committee, Bushehr University of Medical Sciences, Bushehr, Iran
5- Department of Anatomical Sciences, School of Medical Sciences, Bushehr University of Medical Sciences, Bushehr, Iran
Student Research Committee, Bushehr University of Medical Sciences, Bushehr, Iran ,
Abstract: (9 Views)
Background: Mesenchymal stem cells (MSCs) are promising candidates for tissue engineering due to their differentiation potential and ability to secrete growth factors. However, poor survival and proliferation of these cells after transplantation remain a significant challenge. Aphanisomenone flos-aqua (AFA), a green alga, exhibits antioxidant, anti-inflammatory and anti-apoptotic properties. Biodegradable scaffolds with their permeability and water absorption properties provide a suitable platform for cell attachment.
Materials and Methods: First, a scaffold was made according to the protocol. Cells derived from bone marrow waste were separated and cultured, and used at passage three. The groups studied included: a control group without treatment (Control), an MSCs group treated with AFA extract, an MSCs group loaded on the scaffold (PCL/GEL), and an MSCs group loaded on the scaffold with AFA extract (PCL/GEL/AFA). Scanning electron microscope images was used to examine cell attachment on the scaffold. Cell viability and proliferation were determined by the MTT method in each group.
Results: The scaffold’s water absorption and permeability were confirmed. Flow cytometry was positive for CD90, CD73 and CD105 markers and negative for CD44, CD20, CD14 and CD34 markers. Morphological examination revealed a uniform and porous structure. After 72 hours of culture, a significant enhanced cell attachment and proliferation was observed in the groups with scaffolds (P<0.05). Furthermore, a significant increase in cell survival and proliferation was also observed in the groups treated with AFA alcoholic extract (P<0.05). the combination of AFA and PCL/Gel scaffold resulted in significantly higher cell proliferation and survival compared with all other groups (P<0.05).
Conclusion: Our findings suggest that the combination of PCL/Gel nanocomposite and AFA improved the adhesion and survival of human mesenchymal stem cells. Further research is warranted to optimize efficacy of AFA and scaffold design.
Materials and Methods: First, a scaffold was made according to the protocol. Cells derived from bone marrow waste were separated and cultured, and used at passage three. The groups studied included: a control group without treatment (Control), an MSCs group treated with AFA extract, an MSCs group loaded on the scaffold (PCL/GEL), and an MSCs group loaded on the scaffold with AFA extract (PCL/GEL/AFA). Scanning electron microscope images was used to examine cell attachment on the scaffold. Cell viability and proliferation were determined by the MTT method in each group.
Results: The scaffold’s water absorption and permeability were confirmed. Flow cytometry was positive for CD90, CD73 and CD105 markers and negative for CD44, CD20, CD14 and CD34 markers. Morphological examination revealed a uniform and porous structure. After 72 hours of culture, a significant enhanced cell attachment and proliferation was observed in the groups with scaffolds (P<0.05). Furthermore, a significant increase in cell survival and proliferation was also observed in the groups treated with AFA alcoholic extract (P<0.05). the combination of AFA and PCL/Gel scaffold resulted in significantly higher cell proliferation and survival compared with all other groups (P<0.05).
Conclusion: Our findings suggest that the combination of PCL/Gel nanocomposite and AFA improved the adhesion and survival of human mesenchymal stem cells. Further research is warranted to optimize efficacy of AFA and scaffold design.
Keywords: Aphanizomenon flos, Algae, Mesenchymal stem cells, Polycaprolactone, Proliferation, Scaffold
Type of Study: Original |
Subject:
Biotechnology of the sea
Received: 2025/12/9 | Accepted: 2026/05/19 | Published: 2026/06/14
Received: 2025/12/9 | Accepted: 2026/05/19 | Published: 2026/06/14
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