[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 15, Issue 1 (Iranian South Medical of Journal 2012) ::
Iran South Med J 2012, 15(1): 1-12 Back to browse issues page
Comparison of Mycobacterium tuberculosis Beijing genotype with other Mycobacterium tuberculosis strains Using MIRU-VNTR method
Mohaddese Mozafari 1, Parisa Farnia2, Mehdi Jafarian2, Mohammadreza Razavi Deligani3, Mehdi Kazempour2, Mohammadreza Masjedi2, Ali Akbar Velayati2
1- Mycobacteriology research center (MRC), Shahid Beheshty University of Medical Sciences,‌Tehran, IRAN , mohadeseh.mozafari@yahoo.com
2- Mycobacteriology research center (MRC), Shahid Beheshty University of Medical Sciences,‌Tehran, IRAN
3- Islamic Azad University of Qom, Tehran, IRAN
Abstract:   (10175 Views)

Background: Recently it is shown that Mycobacterium tuberculosis Beijing genotype are associated with drug resistance. Thus a simple and rapid method is required for identification and differentiation of Beijing family isolates. Therefore the aim of this study is to evaluate discriminatory power of different loci in both 12 MIRU-VNTR and 15 MIRU-VNTR Methods for Beijing genotype. Methods: In total 105 Mycobacterium tuberculosis strains were identified by spoligotyping, then genomic analysis was carried out by using 12 and 15 MIRU-VNTR methods. Allelic diversity for each locus was calculated using Hunter Gaston Discriminatory Index (HGI). Results: With spoligotyping 20 out of 105 isolates (19.05%) belonged to the Beijing family. Investigating all strains with 12 and 15 MIRU-VNTR showed that 11 loci were highly discriminative (HGI≥0.6) and QUB26 had the highest discriminatory power (HGI=0.84). Number of repeats in QUB11b was specific for Beijing genotype, and only MIRU16 was highly discriminator for this genotype. QUB26, Mtub21, MIRU39, MIRU27, MIRU23 and MIRU26 were moderately discriminator (0.4≤HGI<0.6) and other loci were poorly discriminator for Beijing strains (HGI<0.4). Conclusion: 12 and 15 locus MIRU-VNTR are simple and rapid methods and suitable for differentiation of Beijing genotype from other MTB strains, however these two methods are not suitable for discriminating Beijing family among themselves. In overall discriminatory power of 15 locus MIRU-VNTR method was higher than 12 locus MIRU-VNTR.

Keywords: MIRU-VNTR, mycobacterium tuberculosis, Beijing genotype, tuberculosis
Full-Text [PDF 727 kb]   (2722 Downloads)    
Type of Study: Original | Subject: General
Received: 2012/01/16 | Accepted: 2012/01/16 | Published: 2012/01/16
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Mozafari M, Farnia P, Jafarian M, Razavi Deligani M, Kazempour M, Masjedi M et al . Comparison of Mycobacterium tuberculosis Beijing genotype with other Mycobacterium tuberculosis strains Using MIRU-VNTR method. Iran South Med J. 2012; 15 (1) :1-12
URL: http://ismj.bpums.ac.ir/article-1-308-en.html


Volume 15, Issue 1 (Iranian South Medical of Journal 2012) Back to browse issues page
دانشگاه علوم پزشکی بوشهر، طب جنوب ISMJ

Iranian South Medical Journal is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License which allows users to read,
copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly

Copyright © 2017, Iranian South Medical Journal| All Rights Reserved

Persian site map - English site map - Created in 0.06 seconds with 32 queries by YEKTAWEB 4136