[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 15, Issue 3 (Iranian South Medical Journal 2012) ::
Iran South Med J 2012, 15(3): 183-192 Back to browse issues page
Molecular identification of oxalate-degrading lactobacillus in patients with calcium oxalate urolithiasis
Mohammad Kargar 1, Rouhi Afkari2, Reza Inallo3, Mehdi Kargar4, Sadegh Ghorbani-Dalini5
1- Jahrom Branch, Islamic Azad University, Jahrom, IRAN , mkargar@jia.ac.ir
2- Jahrom Branch, Islamic Azad University, Jahrom, IRAN
3- Jahrom University of Medical Sciences, Fars, IRAN
4- Science and Research Branch, Islamic Azad University, Fars, IRAN
5- Jahrom Branch, Young Researcher’s Club, Islamic Azad University, Jahrom, IRAN
Abstract:   (9702 Views)

Background: Excessive use of diets with high level of oxalate causes an increase in urinary oxalate and leads to the formation of oxalate calcium stone. The aim of this study was evaluation and isolation of an oxalate-degrading bacterium in patients with kidney calcium oxalate stone. Materials and Methods: This cross sectional-descriptive study was carried out on stool and urine samples of 100 healthy individuals and 100 patients with calcium oxalate stone disease in Motahari hospital of Jahrom. The Oxalate-degrading bacteria enriched and cultured in specific medium and were identified by using biochemical tests and 16S rRNA molecular method. Moreover, the presence of oxc and frc genes in isolated bacteria was detected. Results: Lactobacilli were isolated from 80% of healthy individuals and 48% of patients with calcium oxalate stone disease. In 60(46.88%) cases of lactobacilli, oxc gene and 14 (10.94%) cases, frc genes were identified. There was a significant difference between level of urinary oxalate in patients with kidney stone and healthy individuals. But, oxalate – degrading lactobacilli colonization were significantly higher in healthy individuals in comparison with patients with kidney stone. Conclusion: Since the reduction of oxalate- degrading bacteria is one of the reasons of hyperoxaluria and urolithiasis, using these bacteria is recommended for reducing kidney stones.

Keywords: oxalate-degrading bacteria, lactobacilli, calcium oxalate, kidney stone
Full-Text [PDF 402 kb]   (1585 Downloads)    
Type of Study: Original | Subject: General
Received: 2012/06/17 | Accepted: 2012/06/17 | Published: 2012/06/17
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Kargar M, Afkari R, Inallo R, Kargar M, Ghorbani-Dalini S. Molecular identification of oxalate-degrading lactobacillus in patients with calcium oxalate urolithiasis. Iran South Med J. 2012; 15 (3) :183-192
URL: http://ismj.bpums.ac.ir/article-1-333-en.html


Volume 15, Issue 3 (Iranian South Medical Journal 2012) Back to browse issues page
دانشگاه علوم پزشکی بوشهر، طب جنوب ISMJ

Iranian South Medical Journal is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License which allows users to read,
copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly

Copyright © 2017, Iranian South Medical Journal| All Rights Reserved

Persian site map - English site map - Created in 0.06 seconds with 32 queries by YEKTAWEB 4050