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:: Volume 16, Issue 1 (Iranian South Medical Journal 2013) ::
Iran South Med J 2013, 16(1): 1-8 Back to browse issues page
PapG Gene cloning, Escherichia coli uropathogen and examination of its subsequence diversity
Faezeh Hamidiyeh1, Mohammad Hassan Shirazi 2, Jalil Fallah Mehrabadi3, Mohammadreza Pourmand4, Samaneh Ostad Mohammadi1, Hedrousha Molla agha Mirzaei1, Davood Afshar4
1- Department of Microbiology, School of Basic Sciences & Medicine, Islamic Azad University, Zanjan Branch, Zanjan, IRAN
2- Department of Microbiology, School of Health, Tehran University of Medical Sciences, Tehran, IRAN , shirzadeh@yahoo.com
3- Department of Genetic Engineering, Faculty of Biosciences and Biotechnology, Malekashtar University of Technology, Tehran, IRAN
4- Department of Microbiology, School of Health, Tehran University of Medical Sciences, Tehran, IRAN
Abstract:   (9214 Views)

infections are one of the most prevalent human infections. Despite different antigens and toxins of interfering bacteria in infection, one of the important agents in the infections arising from Escherichia coli and the other gram negative bacteria is bacterial binding to host cell surface, so inhibiting the bacterial binding is an appropriate strategy to inhibit the infection. Whereas PapG protein acts as adhesion, it can be an appropriate candidate for developingvaccine. Material and Methods: A Genomic DNA of Escherichia coli bacterium extracted from clinical strain containing PapGII gene. Upon designing primer for PapGII gene, the PCR reaction was applied. The product of PCR was cloned in pBluescript (SK-) plasmid. Using Clustal W and MEGA4 software, the gained subsequence was alignmented with the gene subsequence existing in gene bank and its gene diversity was studied. Results: Based on was down alignment, N terminal on the protein surface and DNA are protected. Conclusion: N terminal domain of PapG gene is a conserved sequence among clinical straines? And it could be used for designing a vaccine against urinary tract infection.

Keywords: Escherichia coli uropathogens, type P pili, gene, cloning
Full-Text [PDF 307 kb]   (2620 Downloads)    
Type of Study: Original | Subject: General
Received: 2012/12/31 | Accepted: 2012/12/31 | Published: 2012/12/31
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Hamidiyeh F, Shirazi M H, Fallah Mehrabadi J, Pourmand M, Ostad Mohammadi S, Molla agha Mirzaei H et al . PapG Gene cloning, Escherichia coli uropathogen and examination of its subsequence diversity. Iran South Med J. 2013; 16 (1) :1-8
URL: http://ismj.bpums.ac.ir/article-1-385-en.html


Volume 16, Issue 1 (Iranian South Medical Journal 2013) Back to browse issues page
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