[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 17, Issue 2 (Iranian South Medical journal 2014) ::
Iran South Med J 2014, 17(2): 182-190 Back to browse issues page
Isolation and sequence analysis of hemagglutinin gene of Influenza A H1N1 virus from Iranian clinical samples during 2009 pandemic flu
Seyyedeh Fahimeh Mousavi1, Fatemeh Fotouhi2, Atena Yousefi1, Behrokh Frarahmand1, Behnaz Heydarchi1, Rezvan Tavakoli1, Masoomeh Tavassoti Kheiri1
1- Department of Virology, Influenza Research Lab, Pasteur Institute of Iran, Tehran, IRAN
2- Department of Virology, Influenza Research Lab, Pasteur Institute of Iran, Tehran, IRAN , fotouhi@pasteur.ac.ir
Abstract:   (6201 Views)

Background: Influenza virus is a globally important respiratory pathogen causing high degree of morbidity and mortality annually. The novel Influenza virus (A/H1N1) which involved many populations of the world in 2009 is a sort of triple reassortment between swine, bird and human viruses. Given the important role of hemagglutinin in the infectivity of influenza virus, genome sequencing of this protein and investigation of its changes seems necessary. Material and Method: In this experimental study, the viral genome was extracted from clinical throat swab samples, in which the presence of swine influenza genome has been confirmed by Real-time PCR according to WHO protocol in Influenza Research Lab, Pasteur Institute of Iran. Full-length of HA genome was amplified using specific primers by one step-RT-PCR, cloned into pGEM-T Easy vector followed by identification with restriction enzyme analysis and sequencing. Results: Full genome of novel influenza A/H1N1 from clinical samples was amplified by PCR and the expected 1777 bp segment PCR product was visualized by electrophoresis, gel purified, cloned into pGEM-TEasy vector and then sequenced. Analysis of sequencing was accomplished by chromas software (version 1.45-Australia) and the nucleotide sequence data was deposited in GenBank database under the accession number: “HQ419001.1”. Conclusion: The result of sequencing was well-matched with the recommended vaccine strain and other registered sequences in NCBI.

Keywords: Influenza A, Hemagglutinin, Pandemic Flu 2009, sequencing, gene bank
Full-Text [PDF 661 kb]   (2050 Downloads)    
Type of Study: Original | Subject: General
Received: 2012/08/15 | Accepted: 2012/10/13 | Published: 2014/04/14
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Mousavi S F, Fotouhi F, Yousefi A, Frarahmand B, Heydarchi B, Tavakoli R et al . Isolation and sequence analysis of hemagglutinin gene of Influenza A H1N1 virus from Iranian clinical samples during 2009 pandemic flu. Iran South Med J. 2014; 17 (2) :182-190
URL: http://ismj.bpums.ac.ir/article-1-524-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 17, Issue 2 (Iranian South Medical journal 2014) Back to browse issues page
دانشگاه علوم پزشکی بوشهر، طب جنوب ISMJ

Iranian South Medical Journal is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License which allows users to read,
copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly

Copyright © 2022, Iranian South Medical Journal| All Rights Reserved

Persian site map - English site map - Created in 0.05 seconds with 30 queries by YEKTAWEB 4419