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:: Volume 17, Issue 5 (Iranian South Medical Journal 2014) ::
Iran South Med J 2014, 17(5): 815-823 Back to browse issues page
Molecular detection of AdeABC efflux pump genes in clinical isolates of Acinetobacter baumannii and their contribution in imipenem resistance
Ali Reza JaponiNejad1, Masoomeh Sofian2, Ehsanolah Ghaznavi-Rad 3
1- Student research committee, Arak University of Medical Sciences, Arak- IRAN
Department of Medical Microbiology, Faculty of Medicine, Arak University Of Medical Sciences, Arak, IRAN
2- Department of Infectious Disease, Arak University of Medical Sciences, Arak- IRAN
3- Department of Medical Microbiology, Faculty of Medicine, Arak University Of Medical Sciences, Arak, IRAN
Molecular research center, Faculty of medicine, Arak University of Medical Sciences, Arak- IRAN , e.ghaznavirad@arakmu.ac.ir
Abstract:   (4468 Views)

Backgrand: Multi-drug resistance due to Acinetobacter baumannii strains has become a significant challenge. Efflux pump plays a vital role in the development of resistance in this bacteria. The aim of this study was to evaluate the frequency of the AdeABC efflux pump genes and its role in resistance to imipenem in clinical isolates of A.baumannii. Materials and methods: A total of 56 isolates of A.baumannii were collected from different clinical specimens of Valiasr hospital in the Arak –Iran and all isolates were identified by standard biochemical tests. The Antimicrobial susceptibility patterns were determined by disk diffusion method and minimum inhibitory concentrations of imipenemin via E-test strips with and without CCCP efflux pump inhibitor were determined according to CLSI guidelines. The PCR test was used to detect the AdeABC efflux pump genes in isolates. Results: All A.bumannii isolates were resistant to cefotaxim, ceftazidim, cefepim, cefoxitin, azteronam, piperacillin-tazobactam and ciprofloxacin, as well as all isolates were resistant to imipenem according to the results of the E-test method. Imipenem MIC with efflux pump inhibitor not reduced in all isolates and showed no differences in imipenem activity. The adeA, adeB and adeC genes were found in 100%, 100% and 96.5% of isolates, respectively. Conclusion: AdeABC efflux system contributes to resistance to other antibiotics and resistance to imipenem has not been involved with this efflux system in A.baumanni isolates in current study and other mechanism such as carbapenemase enzymes play vital role to imipenem resistance in A.baumanni isolates.

Keywords: Acinetobacter baumannii, AdeABC efflux pump, resistance to imipenem
Full-Text [PDF 609 kb]   (1509 Downloads)    
Type of Study: Original | Subject: General
Received: 2014/10/15 | Accepted: 2014/10/15 | Published: 2014/10/15
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JaponiNejad A R, Sofian M, Ghaznavi-Rad E. Molecular detection of AdeABC efflux pump genes in clinical isolates of Acinetobacter baumannii and their contribution in imipenem resistance. Iran South Med J. 2014; 17 (5) :815-823
URL: http://ismj.bpums.ac.ir/article-1-595-en.html


Volume 17, Issue 5 (Iranian South Medical Journal 2014) Back to browse issues page
دانشگاه علوم پزشکی بوشهر، طب جنوب ISMJ

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