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:: Volume 17, Issue 6 (Iranian South Medical Journal 2015) ::
Iran South Med J 2015, 17(6): 1120-1134 Back to browse issues page
Study of the effect of GABAA receptore and glial inhibition on behavioral responses in CCI model of neuropathic pain in rat
Mehi Sadeghi1, Homa Manaheji 2, Abbad Haghparast3, Jalal Zaringhalam4, Samad Nazemi5, Zahra Bahari6
1- Department of physiology, School of Medicine, Bushehr University of Medical Sciences, Bushehr, IRAN
Department of Neurophysiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IRAN
2- Neuroscice Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IRAN
Department of Neurophysiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IRAN , hshardimanaheji@yahoo.com
3- Neuroscice Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IRAN
4- Neuroscice Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IRAN
Department of Neurophysiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IRAN
5- Department of physiology, School of Medicine, Sabzevar University of Medical Sciences, Sabzevar, IRAN
6- Department of Neurophysiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IRAN
Abstract:   (3566 Views)

Background: The mechanisms underlying neuropathic pain are complex and remain controversial. From the proposed mechanisms we can refer to loss of GABAergic inhibition and glial activation in the spinal dorsal horn. As for the discrepancies in the neuropathic pain mechanisms, in the present study, we examined whether the GABA-A receptor agonist muscimol and glial inhibitor pentoxifylline would modify behavioral tests in rats with Chronic Constriction Injury (CCI).

Material and Methods: In this study male wistar rats (200-250 g) were used and for neuropathy induction, the CCI model (Bennett method, 1988) was used. In the sham group, after exposing the sciatic nerve, surgery region was closed. Pentoxifylline was administered one day before neuropathy to 14 days after CCI (30 mg/kg daily) and behavioral tests (plantar test and von frey) were performed one day before surgery and then on days 1, 4, 7 and 14 after surgery and 30 minutes after pentoxifylline adminstration .In groups that received muscimol with doses 0.5, 1 and 2 mg/kg on day 14 after CCI, behavioral tests were experienced before and 30 minutes after drug administration.

Results: Behavioral assessment indicated that CCI induce symptoms of neuropathic pain but both muscimol and pentoxifylline could reduce pain behavioral responses. It seems that this reduction of muscimol (1 and 2 mg/kg) was more effective in thermal hyperalgesia than pentoxifylline, and for pentoxifylline (30 mg/kg) was more effective in mechanical allodynia than muscimol. Conclusion: present data showed that muscimol via activation of GABA-A receptors and pentoxifylline via glial inhibition reduced behavioral symptoms of neuropathic pain after spinal cord injury.

Keywords: neuropathic pain, hyperalgesia, allodynia, CCI, muscimol, pentoxifylline
Full-Text [PDF 595 kb]   (1168 Downloads)    
Type of Study: Original | Subject: Nervous System
Received: 2014/12/18 | Accepted: 2014/12/18 | Published: 2014/12/18
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Sadeghi M, Manaheji H, Haghparast A, Zaringhalam J, Nazemi S, Bahari Z. Study of the effect of GABAA receptore and glial inhibition on behavioral responses in CCI model of neuropathic pain in rat. Iran South Med J. 2015; 17 (6) :1120-1134
URL: http://ismj.bpums.ac.ir/article-1-628-en.html


Volume 17, Issue 6 (Iranian South Medical Journal 2015) Back to browse issues page
دانشگاه علوم پزشکی بوشهر، طب جنوب ISMJ

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