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:: Volume 8, Issue 2 (Iranian south medical of journals 2006) ::
Iran South Med J 2006, 8(2): 103-109 Back to browse issues page
Identification of Helicobacter pylori DNA in gallstones using Multiplex PCR
Shohreh Farshad Ms *1, Abdolvahab Alborzi Mr2, Ali Malek Hossaini Mr2, Aziz Japooni Mr3, Barat Aboodi Mr4, Jalil Nasiri Mr5
1- Assistant Professor , s_farshad@yahoo.com
2- Professor
3- Assistant Professor
4- Lecturer
5- BSC
Abstract:   (13664 Views)
Background: Recent studies showed a possible relationship between infections caused by some of Helicobacter members and gallstones formation. The aim of this study was identification of Helicobacter members in gallstones from patients with biliary diseases. Methods: Gallstones and bile samples from 33 patients were subjected to rapid urease test, culture and Multiplex-PCR using primers based on 16s rRNA and isocitrate dehydrogenase genes to identify Helicobacter genus and H. pylori species genes, respectively. This PCR was also done on bile samples from 40 autopsied gallbladders with normal pathology (as a control group). Results: In 18.1% of stones and 12.1% of bile samples, H. pylori DNA was detected using PCR. Rapid urease and cultures tests were negative for all samples. The genome of H. pylori was not detected in control group using PCR. Conclusion: H. pylori DNA was detected in gallstone, however, we are not sure of H. pylori viability in these samples. To clarify the clinical role of Helicobacter in gallbladder diseases, more investigations are needed to ascertain whether this microorganism is innocent bystander or active participant in gallstone formation.
Keywords: helicobacter pylori, bile, gallstone, Multiplex-PCR
Full-Text [PDF 6527 kb]   (1644 Downloads)    
Type of Study: Original | Subject: General
Received: 2009/01/14 | Accepted: 2009/01/14 | Published: 2009/01/14
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Farshad S, Alborzi A, Malek Hossaini A, Japooni A, Aboodi B, Nasiri J. Identification of Helicobacter pylori DNA in gallstones using Multiplex PCR. Iran South Med J. 2006; 8 (2) :103-109
URL: http://ismj.bpums.ac.ir/article-1-64-en.html

Volume 8, Issue 2 (Iranian south medical of journals 2006) Back to browse issues page
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