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:: Volume 18, Number 6 (Iranian South Medical Journal 2016) ::
Iran South Med J 2016, 18(6): 1140-1148 Back to browse issues page
Cloning and sequencing of the gene encoding LipL21 in the vaccinal leptospira serovars
Rasoul Hoseinpur1, Pezhvak Khaki *2, Soheila Moradi Bidhendi1, Mojtaba Noofeli3
1- Leptospira Reference laboratory, Department of Microbiology, Razi Vaccine Research Institute, Karaj, Iran
2- Leptospira Reference laboratory, Department of Microbiology, Razi Vaccine Research Institute, Karaj, Iran , P.Khaki@rvsri.ac.ir
3- Human Bacterial Vaccines Research and Production Department, Razi Vaccine Research Institute, Karaj, Iran
Abstract:   (1394 Views)

Background: Leptospirosis is a zoonotic disease in humans and animals, caused by the bacterium Leptospira interrogans. Gene expressing LipL21 is one of the genes identified in the bacterium, existing only in the pathogenic strains. The aim of this study was to cloning and analyzing the sequence of the gene encoding surface lipoprotein, LipL21, in five vaccinal leptospira serovars in Iran.

Material and Methods: Pathogenic Leptospira interrogans serovars were cultured in EMJH medium with 10% rabbit serum. After genomic DNA extraction, PCR with specific primers was employed and the resulting product inserted in a vector then transferred into E. Coli DH5&alpha. The recombinant plasmids were finally sent for sequencing.

Results: The analysis of gene lipL21 in domestic vaccinal serovars and comparison of them with other serovars in the GenBank database revealed that three vaccinal serovars serjo hardjo, canicola and pomona had 100% similarity with each other and grippotyphosa serovar had the highest difference with the vaccinal serovars. In general, the results showed that this gene is a highly conserved gene in the domestic vaccinal serovars and serovars in the GenBank database with more than 95.7 percent similarity.

Conclusion: These results showed that the gene, lipL21, is highly conserved in the vaccinal serovars (similarities > 96.4 %). Therefore, the gene encoding surface protein LipL21 can serve as a useful serologic test with high specificity and sensitivity for diagnosis of leptospirosis in clinical samples and in future as an effective subunit vaccine candidate to be used.

Keywords: Leptospirosis, Leptospira Spp., lipL21 gene, cloning
Full-Text [PDF 636 kb]   (844 Downloads)    
Type of Study: Original | Subject: Microbiology and Immunology
Received: 2015/12/22 | Accepted: 2015/12/22 | Published: 2015/12/22
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DOI: 10.7508/ismj.1394.06.004


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Hoseinpur R, Khaki P, Moradi Bidhendi S, Noofeli M. Cloning and sequencing of the gene encoding LipL21 in the vaccinal leptospira serovars. Iran South Med J. 2016; 18 (6) :1140-1148
URL: http://ismj.bpums.ac.ir/article-1-747-en.html
Volume 18, Number 6 (Iranian South Medical Journal 2016) Back to browse issues page
دانشگاه علوم پزشکی بوشهر، طب جنوب ISMJ

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