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:: Volume 18, Number 6 (Iranian South Medical Journal 2016) ::
Iran South Med J 2016, 18(6): 1149-1163 Back to browse issues page
Effect of nitroprusside on furosemide-induced skeletal teratogenicity in rat fetuses
Mahmood Khaksary Mahabady *1, Hosean Najafzadeh Varzi2, Mozhgan Sabet3
1- Department of Anatomy and Embryology, Faculty of Veterinary Medicine, ShahidChamran University, Ahvaz, Iran , mkhaksarymahabady@yahoo.com
2- Department of Pharmacology, Faculty of Veterinary Medicine, ShahidChamran University, Ahvaz, Iran
3- Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran
Abstract:   (1227 Views)

Background: Furosemide as a loop diuretic can use in treatment of hypertension, renal or heart failures and cirrhosis, when sodium retention is significant. It is known that use of furosemide can be lead congenital abnormalities in humans and animals. Nitroprusside as a NO donor can decrease blood supply complications and constriction of placenta and uterus via vasodilation and improvment blood supply. The aim of this study was preventation or decrease of teratogenicity form furosemide in rat fetuses by sodium nitroprusside.

Materials and Methods: This study was performed on 28 pregnant rats that were divided into four groups, the groups consist control, furosemide, sodium nitroprusside and furosemide plus sodium nitroprusside. Drugs were administrated on 14th and 16th day of gestation. Test groups received furosemide (200mg/kg) orally, and nitroprusside (0.5 mg/kg) intraperitoneally. The rats were euthanized and fetuses were collected at 19th day of gestation, after weight and length determination, they stained by Alizarin red- Alician blue method. Then the skeletal system of the stained fetuses was investigated by stereomicroscope for teratogenicity effects.

Results: The results showed the cleft palate, wavy ribs and decreased ossification mean incidence in forelimbs and hindlimbs were 11.11%, 68.88% and 20% in the fetuses of the rats received furosemide, where as it decreased to 7.31%, 21.95% and 12.19% in group which received furosemide plus nitroprusside, respectively.

Conclusion: It is concluded that sodium nitroprusside can significantly decrease teratogenicity induced by furosemide.

Keywords: Teratogenicity, Furosemide, Nitroprusside, Rat, Skeletal system
Full-Text [PDF 962 kb]   (399 Downloads)    
Type of Study: Original | Subject: Musculoskeletal System
Received: 2015/12/22 | Accepted: 2015/12/22 | Published: 2015/12/22
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DOI: 10.7508/ismj.1394.06.005


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Khaksary Mahabady M, Najafzadeh Varzi H, Sabet M. Effect of nitroprusside on furosemide-induced skeletal teratogenicity in rat fetuses. Iran South Med J. 2016; 18 (6) :1149-1163
URL: http://ismj.bpums.ac.ir/article-1-748-en.html
Volume 18, Number 6 (Iranian South Medical Journal 2016) Back to browse issues page
دانشگاه علوم پزشکی بوشهر، طب جنوب ISMJ

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