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:: Volume 18, Number 6 (Iranian South Medical Journal 2016) ::
Iran South Med J 2016, 18(6): 1198-1207 Back to browse issues page
Molecular Typing of Pseudomonas aeruginosa Strains Isolated from Burn Patients in South of Iran
Aziz Japoni1, Mojtaba Anvarinejad *2, Jalal Mardaneh3
1- Professor Alborzi Clinical Microbiology Research Center, Nemazee Hospital, Shiraz University of Medical Sciences, Shiraz, Iran
2- Professor Alborzi Clinical Microbiology Research Center, Nemazee Hospital, Shiraz University of Medical Sciences, Shiraz, Iran , anvarinejad@yahoo.com
3- Department of Microbiology, School of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran
Abstract:   (1143 Views)

Background: Pseudomonas aeruginosa is one of the main etiological agents in burn infections which could be life threatening for the infected patients. The aim of the present study was to identify and track source of infections using two molecular typing methods.

Materials and Methods: Seventy-four strains of P. aeruginosa were isolated from burn patients and hospital environment in Ghotbadden Burn Hospital, Shiraz, Iran. Isolates were typed by arbitrary primed-polymerase chain reaction (AP-PCR) and plasmid profiling. Similarity and clustering of the strains was assessed using NTSYS-PC software and photo Capt Mw program.

Results: Thirty eight plasmid profiles were obtained and classified them into: 2, 3and 5 clusters, based on 50%, 64.7% and 67.5% similarity on the plotted dendrogram, respectively. Drawn dendrogarm categorized AP-PCR products to 47 different types.

Conclusion: Based on these results, a limited number of P. aeruginosa types are predominant in the hospitals which infect the burn patients. To control of the infections in patients with antibiotics, resistant isolates, strong disinfection of patients’ bathroom after scrubbing of patients wounds, should be implemented.

Keywords: Pseudomonas aeruginosa, Plasmid typing, Source of infections, AP-PCR.
Full-Text [PDF 655 kb]   (450 Downloads)    
Type of Study: Original | Subject: Dermatology
Received: 2015/12/22 | Accepted: 2015/12/22 | Published: 2015/12/22
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DOI: 10.7508/ismj.1394.06.010


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Japoni A, Anvarinejad M, Mardaneh J. Molecular Typing of Pseudomonas aeruginosa Strains Isolated from Burn Patients in South of Iran. Iran South Med J. 2016; 18 (6) :1198-1207
URL: http://ismj.bpums.ac.ir/article-1-753-en.html
Volume 18, Number 6 (Iranian South Medical Journal 2016) Back to browse issues page
دانشگاه علوم پزشکی بوشهر، طب جنوب ISMJ

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