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:: Volume 19, Number 2 (Iranian South Medical Journal 2016) ::
Iran South Med J 2016, 19(2): 185-193 Back to browse issues page
Expression of apoptosis-Related genes bcl-2 and bax in rat brain hippocampus, followed by intraperitoneal injection of nanosilver
Maryam Ghooshchian1, Parvin Khodarahmi *2, Farzaneh Tafvizi1
1- Department of biology, Branch of Parand, Islamic Azad University, Parand, Iran
2- Department of biology, Branch of Parand, Islamic Azad University, Parand, Iran , khodarahmiparvin@yahoo.com
Abstract:   (2441 Views)

Background: Silver nanoparticles are small scale substance (<100 nm) used in food technology and medical industry. The data suggest that nanosilver may produce neurotoxicity by generating free radical-induced oxidative stress and by altering gene expression producing apoptosis and neurotoxicity. In this study, the apoptotic effects of Nano silver on apoptosis- related genes expression bcl-2 and bax on rat hippocampus, which is involved in memory and learning, was investigated.

Materials & Methods: 28 male Wistar rats were divided into four groups of control and three groups of the treatment. The control group received saline and the treatment groups received intraperitoneal injections of silver nanoparticles at doses of 100, 200 and 400ppm. Ten days after the last injection, the hippocampal region was dissected and removed and then the expression of bcl-2 and bax genes was evaluated using semi-qualitative RT-PCR and Densitometry assay.

Results: The expression of anti- apoptotic b-cl2 gene was reduced in the treatment groups compared to the control group. In comparison, the expression of pro- apoptotic bax gene was increased in the treatment groups compared to the control group. This apoptotic affects was increased at higher doses.

Conclusion: The data suggest that silver nanoparticles may produce apoptosis by altering apoptosis- related genes expression, in rat brain hippocampus cells.

Keywords: silver nanoparticles, bcl-2 gene, bax gene, semi-qualitative RT-PCR, Densitometry
Full-Text [PDF 712 kb]   (767 Downloads)    
Type of Study: Original | Subject: Nervous System
Received: 2016/05/17 | Accepted: 2016/05/17 | Published: 2016/05/17
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DOI: 10.18869/acadpub.ismj.19.2.185


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Ghooshchian M, Khodarahmi P, Tafvizi F. Expression of apoptosis-Related genes bcl-2 and bax in rat brain hippocampus, followed by intraperitoneal injection of nanosilver. Iran South Med J. 2016; 19 (2) :185-193
URL: http://ismj.bpums.ac.ir/article-1-782-en.html
Volume 19, Number 2 (Iranian South Medical Journal 2016) Back to browse issues page
دانشگاه علوم پزشکی بوشهر، طب جنوب ISMJ

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