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:: Volume 19, Issue 4 (Iranian South Medical Journal 2016) ::
Iran South Med J 2016, 19(4): 629-643 Back to browse issues page
Association between COX-2 A1195G polymorphism with migraine in patients with consanguineous marriage of parents
Elahe Mozaffari 1, Mostafa Faghani 2, Reza Nemati 3, Morteza Makhlooei 4
1- Department of Genetics, Faculty of Sciences, Islamic Azad University of Shahrekord branch, Shahrekord, Iran , elahehsea@yahoo.com
2- Department of Animal Sciences and Fisheries, Faculty of Agricultural Sciences, Islamic Azad University of Shahrekord branch, Shahrekord, Iran
3- Department of Neurology, School of Medicine, Boushehr University of Medical Sciences, Boushehr,Iran
4- Department of Fishery, Faculty of Agricultural Sciences and Natural Resources, Islamic Azad University of Boushehr branch, Boushehr, Iran
Abstract:   (2011 Views)

Background: Migraine is a common debilitating headache with current head pain attacks which associated with temporal changes of head blood vessels diameter and  has been classified into two main categories, migraine with aura (MA) and migraine without aura (MO) by the International criteria for Headache Society (IHS). This study was performed with the aim of studying the association of COX-2-1195A G gene polymorphism, risk of migraine susceptibility and  it’s relation with parent marriage type in two control and case groups.

Materials and Methods: Genomic DNA of blood samples was purified from 100 migraine cases and 100 controls in this study. By using the appropriative COX-2-1195A→G (rs89466) primer and Pvu II restriction enzyme in PCR- RFLP manner the expected region of subject’s COX-2 gene was amplified and digested.

Results: After analysising the data with SPSS twentieth version software, it was observed that frequency of COX-2-1195 GG and COX-2-1195 AG genotypes carriers in patients were higher than in the controls (9 percent and 41 percent in migraine cases, 5 percent and 24 percent in controls respectively; (P>0.010)), also it was specificated that frequences of mentioned genotypes has been significantly higher in patients with relative parent than in control group (8.1 percent and 48.6 percent in cases with relative parent, 5 percent and 24 percent in controls respectively; (P>0.011)).

Conclusion: Regarding high frequency of polymorph allele (G) in between patients with consanguineous parents, it can be resulted that consanguineous marriage increase the risk of this allele incidence and migraine outbreak. So, further studies with larger sample groups are needed on different nations of other regions to achieve better results about genetic of migraine, especially COX-2 gene.

Keywords: Migraine, Polymorphism, COX-2 gene, consanguineous marriage
Full-Text [PDF 357 kb]   (730 Downloads)    
Type of Study: Original | Subject: Biochemistry. Cell Biology and Genetics
Received: 2016/09/7 | Accepted: 2016/09/7 | Published: 2016/09/7
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Mozaffari E, faghani M, Nemati R, Makhlooei M. Association between COX-2 A1195G polymorphism with migraine in patients with consanguineous marriage of parents. Iran South Med J. 2016; 19 (4) :629-643
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Volume 19, Issue 4 (Iranian South Medical Journal 2016) Back to browse issues page
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