Volume 19, Issue 5 (Iranian South Medical Journal 2016)                   Iran South Med J 2016, 19(5): 773-786 | Back to browse issues page

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Behruzi M, Ghasemi Hamidabadi H, Gholipour Malekabadi M, Rezaei N, Nazm Bojnordi M, Malekzadeh Shafaroudi M. Using Hydroxyapatite-Gelatin Scaffold Seeded with Bone Marrow Stromal Cells as a Bone Graft in Animal Model. Iran South Med J 2016; 19 (5) :773-786
URL: http://ismj.bpums.ac.ir/article-1-826-en.html
Using Hydroxyapatite-Gelatin Scaffold Seeded with Bone Marrow Stromal Cells as a Bone Graft in Animal Model
Mahsoumeh Behruzi1 , Hatef Ghasemi Hamidabadi1 , Mazaher Gholipour Malekabadi2 , Noorollah Rezaei1 , Maryam Nazm Bojnordi1 , Majid Malekzadeh Shafaroudi * 3
1- Immunogenetic Research Center (IRC), Department of Anatomy & Cell Biology, .School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
2- Biotechnology Department, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3- Immunogenetic Research Center (IRC), Department of Anatomy & Cell Biology, .School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran , malek1344@gmail.com
Abstract:   (8245 Views)

Background: Nowadays, composite scaffolds with some desired characteristics have a numerous applications in hard tissue engineering. In present study, the role of composite hydroxyapatite - gelatin was examined in both alone and coated by Bone Marrow Stromal Stem Cells (BMSCs) conditions in the process of healing bone defects, reduction of time repair and the immune response of body by laboratory studies (in vitro) and in vivo on the skull of adult rats as well.

Materials and Methods: In present study, nano-hydroxyapatite powder and gelatin were used to provide nano-hydroxyapatite-gelatin scaffold, BMSCs were isolated by Flushing method. Fifteen adult male Wistar rats weighing 250-200 g were used. Studing groups included bone defect with hydroxyapatite-gelatin scaffold, bone defect with hydroxyapatite-gelatin with BMSCs and bone defects without scaffolding as a controlwhich were examined after a week and a month after surgery. MTT assay was used in order to evaluation of biocompatibility of scaffolds. To confirm the healing progress trend and the presence of inflammatory cells we used hematoxylin-eosin and we used Masson's trichrome staining in order to study of synthesis of collagen fibers.

Results: The results of MTT showed that the scaffold has no toxic effects on stromal cells. The first signs of ossification in hydroxyapatite-gelatin with BMSCs cells group, appeared in the first week. However, in the fourth week, ossification was completed and the scaffold remaining was found as embedded islands in the spongy bone tissue. The greatest number of lymphocytes was observed in the experimental group after one week of planting scaffold.

Conclusion: it seems that Hydroxyapatite-gelatin scaffold coated with BMSCs cells has a potential role in the healing process of bone and it can be suitable as a therapeutic strategy to repair extensive bone lesions.

Keywords: Transplantation, Scaffold, Rat, Bone marrow mesenchymal stem cell, Tissue engineering
Full-Text [PDF 583 kb]   (5124 Downloads)    
Type of Study: Original | Subject: Musculoskeletal System
Received: 2015/08/31 | Accepted: 2015/11/23 | Published: 2016/11/17
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