Volume 19, Issue 6 (Iranian South Medical Journal 2017)                   Iran South Med J 2017, 19(6): 931-939 | Back to browse issues page

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Salehi Z, Gholaminia Z, Panjtanpanah M R. Association of HSP70-2 Gene 1267A/G Polymorphism With Cataract Incidence Among Guilan Population. Iran South Med J 2017; 19 (6) :931-939
URL: http://ismj.bpums.ac.ir/article-1-841-en.html
Association of HSP70-2 Gene 1267A/G Polymorphism With Cataract Incidence Among Guilan Population
Zivar Salehi * 1, Zahra Gholaminia2 , Mohammad Reza Panjtanpanah3
1- Department of Biology, School of Sciences, University of Guilan, Rasht, Iran , geneticzs@yahoo.co.uk
2- Department of Biology, School of Sciences, University of Guilan, Rasht, Iran
3- Department of Ophthalmology, School of medicine, Guilan University of Medical Sciences, Rasht, Iran
Abstract:   (8412 Views)

Background: Cataract is a visible opacity of the eye lens and it is the main reason of reversible blindness in the world. Oxidative stress is known as a major factor in cataract formation HSP70-2 protein is a molecular chaperone which is essential for cell survival in stress conditions. HSP70-2 gene is located in the human leukocyte antigen class ΙΙΙ region. This gene encodes an inducible protein. One of the common polymorphism of HSP70-2 is 1267A/G which is located in coding region. The aim of this study was to analysis of 1267A/G polymorphism of hsp70-2 gene in cataract patients.

Material and Methods: This case-control study included 118 cataract patients and 122 healthy people as a control groups. Genomic DNA was extracted from peripheral blood leukocytes and genotyping determination was performed by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Statistical analysis was performed by using the MedCalc software (Version 12.1).

Results: The frequency of G allele was significantly higher in patients than the controls, (0.36 and 0.24, respectively). A higher frequency of the GG genotype of the HSP70-2 1267A/G polymorphism was found in the patients compared with controls (21.19% and 8.20%, respectively). The patients carrying the GG genotype were 3.2-fold at a higher risk of cataract compared with AA genotype (P=0.005).

Conclusion: The finding of this study suggested that the HSP70-2 1267A/G may affect the susceptibility to cataract in the studied population. Anyway the randomized multicenter studies with greater sample size still need to confirmed our results.

Keywords: Cataract, gene polymorphism, HSP70-2 gene, oxidative stress
Full-Text [PDF 717 kb]   (2649 Downloads)    
Subject: Biochemistry. Cell Biology and Genetics
Received: 2015/08/26 | Accepted: 2015/12/7 | Published: 2016/12/27
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