[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 20, Issue 6 (Iranian South Medical Journal 2018) ::
Iran South Med J 2018, 20(6): 519-526 Back to browse issues page
Analysis of Ala234Thr Polymorphism SEPP1 gene in Women with Breast Cancer in Guilan Province
Samaneh Mohammaddoust 1, Zivar Salehi *2, Hamid Saeidi Saedi 3
1- Department of Biology, School of Sciences, University of Guilan, Rasht, Iran
2- Department of Biology, School of Sciences, University of Guilan, Rasht, Iran , geneticzs@yahoo.co.uk
3- Department of Oncology, Cancer Research Center, Guilan University of Medical Sciences, Rasht, Iran
Abstract:   (296 Views)
Background: Selenoprotein P (Sepp1) is the major selenoprotein in plasma and contains over 50% of the total plasma. The direct and indirect effects of Sepp1 on anti-oxidant defense can be responsible for its role in cancer risk. One of the common polymorphism of SEPP1gene is the Ala234Thr single nucleotide polymorphism (SNP) in coding region. In this research we studied the association between SEPP1 Ala234Thr (rs3877899) polymorphism with the risk of breast cancer.
Materials and Methods: In this case-control study, 162 patients with breast cancer were compared with 227 healthy subjects. Genomic DNA was extracted from peripheral blood leukocytes. Determination of DNA genotyping in SEPP1 Ala234Thr (rs3877899) polymorphism was performed by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) by using MwoI enzyme. Statistical analysis was performed by using the MedCalc program and χ2-test.
Results: The genotype frequencies were significantly different between the cases and controls. AA homozygosity subjects were more at risk for breast cancer than others  (OR=3.23; 95%CI, 1.79-5.84; p=0.001).
Conclusion:Based on the results of this study,  The Ala234Thr polymorphism of the SEPP1 gene may be associated with the potential for breast cancer in the female population of  northern Iranian women. However, studiesneed to be conducted in larger populations to confirm these results.
Keywords: Guilan province, polymorphism, Breast cancer, Selenoprotein, Ala234Thr, SEPP1
Full-Text [PDF 803 kb]   (119 Downloads)    
Type of Study: Original | Subject: General
Received: 2018/01/7 | Accepted: 2018/01/7 | Published: 2018/01/7
Send email to the article author

Add your comments about this article
Your username or Email:

Write the security code in the box >


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Mohammaddoust S, Salehi Z, Saeidi Saedi H. Analysis of Ala234Thr Polymorphism SEPP1 gene in Women with Breast Cancer in Guilan Province. Iran South Med J. 2018; 20 (6) :519-526
URL: http://ismj.bpums.ac.ir/article-1-903-en.html


Volume 20, Issue 6 (Iranian South Medical Journal 2018) Back to browse issues page
دانشگاه علوم پزشکی بوشهر، طب جنوب ISMJ

Iranian South Medical Journal is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License which allows users to read,
copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly

Copyright © 2017, Iranian South Medical Journal| All Rights Reserved

Persian site map - English site map - Created in 0.053 seconds with 804 queries by yektaweb 3600