[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 21, Issue 1 (Iranian South Medical Journal 2018) ::
Iran South Med J 2018, 21(1): 29-39 Back to browse issues page
CFL1 Gene Expression in the Intestinal Samples of Gastric Adenocarcinoma in East Azarbaijan Population
S.Saeadeh Daryabari1 , Reza Safaralizadeh 2, Mohammad Ali Hosseinpour Feizi1, Yaghob Moaddab3, Rehaneh Ravanbakhsh Gavgani1
1- Department of Animal Biology, University of Tabriz, Tabriz, Iran
2- Department of Animal Biology, University of Tabriz, Tabriz, Iran , safaralizadeh@tabrizu.ac.ir
3- Liver and Gastroenterology Diseases Research Center, Tabriz University of Medical Sciences,Tabriz, Iran
Abstract:   (3801 Views)
Background: Gastric cancer is the fourth most common cancer and the second most common cause of cancer-related death around the world. Northern areas of Iran from northwest to northeast have a high risk of this cancer. Genetic mutations and changes in expression level are the most effective factors in carcinogenesis and cancer progression. CFL1 is one of the genes affected by changes in expression level. CFL1 is isoform of the ADF/cofilin protein family. The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. In this research, we studied the expression of this gene in intestinal samples of gastric adenocarcinoma.
Material and Methods: In this study, mRNA was extracted from the intestinal type of gastric adenocarcinoma of 26 biopsy samples. Next, real-time polymerase chain reaction was performed to measure gene expression  after cDNA synthesis.
Results: The rate of CFL1 gene expression in tumor samples significantly increased compared with healthy tumor margins (p<0.05). Furthermore, the biomarker index for CFL1 was obtained 0.88.
Conclusion: This gene can be used as a biomarker in gastric cancer. Overall, the results of this study showed that the expression of CFL1 has a significant association with the nature of cancer tumors. This implies that CFL1 has a high potential as a novel target for gene therapies in gastric cancer.
 
 
 
Keywords: Stomach Neoplasm, Adenocarcinoma, CFL1
Full-Text [PDF 832 kb]   (1485 Downloads)    
Type of Study: Original | Subject: Biochemistry. Cell Biology and Genetics
Received: 2017/02/8 | Accepted: 2017/10/16 | Published: 2018/02/26
References
1. Rahman R, Asombang AW, Ibdah JA. Characteristics of gastric cancer in Asia. World J Gastroenterol 2014; 20(16):4483-90. [DOI:10.3748/wjg.v20.i16.4483]
2. Malekzadeh R, Derakhshan MH, Malekzadeh Z. Gastric cancer in Iran: epidemiology and risk factors. Arch Iran Med 2009;12(6):576-83.
3. Kolahdoozan Sh, Sadjadi AR, Radmard AR, et al. Five common cancers in Iran. Archives of Iranian medicine 2010;13(2):143-6.
4. Azarbarzin SH, Hosseinpour Feizi1 MA, Sa-faralizadeh R, et al. The Value of miR-299-5p in Diagnosis and Prognosis of Intestinal-Type Gastric Adenocarcinoma. Bio-chem Genet 2016; 54(4):413-20 [DOI:10.1007/s10528-016-9728-y]
5. Layke JC, Lopez PP. Gastric cancer: diagnosis and treatment options. American family physician 2004; 69(5): 1133-40.
6. Otterbein LR, Graceffa P, Dominguez R. The crystal structure of uncomplexed actin in the ADP state. Science 2001; 293(5530):708-11. [DOI:10.1126/science.1059700]
7. Vindin H, Gunning P. Cytoskeletal tropomyosins: choreographers of actin filament functional diversity. Journal of muscle research and cell motility 2013; 34(3-4): 261-74. [DOI:10.1007/s10974-013-9355-8]
8. Wang W, Eddy R, Condeelis J. The cofilin pathway in breast cancer invasion and metastasis. Nature Reviews Cancer 2007;7(6):429-40. [DOI:10.1038/nrc2148]
9. Moon AL, Janmey PA, Louie KA, Drubin DG. Cofilin is an essential component of the yeast cortical cytoskeleton. The Journal of Cell Biology 1993; 120(2): 421-35. [DOI:10.1083/jcb.120.2.421]
10. Gunsalus KC, Bonaccorsi S, Williams E, Verni F, Gatti M, Goldberg ML. Mutations in twinstar, a Drosophila gene encoding a cofilin/ADF homologue, result in defects in centrosome migration and cytokinesis. The Journal of cell biology 1995; 131(5): 1243-59 [DOI:10.1083/jcb.131.5.1243]
11. Aizawa H, Sutoh K, Tsubuki S, et al. Identification, characterization, and intracellular distribution of cofilin in Dictyostelium discoideum. Journal of Biological Chemistry 1995; 270(18):10923-32 [DOI:10.1074/jbc.270.18.10923]
12. Kanellos G, Zhou J, Patel H, et al. ADF and Cofilin1 control actin stress fibers, nuclear integrity, and cell survival. Cell reports 2015;13(9):1949-64. [DOI:10.1016/j.celrep.2015.10.056]
13. Vartiainen MK, Mustonen T, Mattila PK, Ojala PJ, Thesleff I, Partanen J, et al. The Three Mouse Actin-depolymerizing Factor/Cofilins Evolved to Fulfill Cell-Type-specific Requirements for Actin Dynamics. Molecular biology of the cell 2002;13(1):183-94. [DOI:10.1091/mbc.01-07-0331]
14. Zuchero JB, Fu M-m, Sloan SA, et al. CNS myelin wrapping is driven by actin disassembly. Developmental cell 2015; 34(2):152-67. [DOI:10.1016/j.devcel.2015.06.011]
15. Hotulainen P, Paunola E, Vartiainen MK, Lappalainen P. Actin-depolymerizing factor and cofilin-1 play overlapping roles in promoting rapid Factin depolymerization in mammalian nonmuscle cells. Molecular biology of the cell 2005;16(2):649-64. [DOI:10.1091/mbc.e04-07-0555]
16. Matsuzaki F, Matsumoto S, Yahara I, et al. Cloning and characterization of porcine brain cofilin cDNA. Cofilin contains the nuclear transport signal sequence. Journal of Biological Chemistry 1988; 263(23): 11564-8.
17. Lanford RE, Kanda P, Kennedy RC. Induction of nuclear transport with a synthetic peptide homologous to the SV40 T antigen transport signal. Cell 1986; 46(4): 575-82. [DOI:10.1016/0092-8674(86)90883-4]
18. Wang W, Mouneimne G, Sidani M, Wyckoff J, Chen X, Makris A, et al. The activity status of cofilin is directly related to invasion, intravasation, and metastasis of mammary tumors. The Journal of cell biology 2006;173(3):395-404. [DOI:10.1083/jcb.200510115]
19. Arber S, Barbayannis FA, Hanser H, et al. Regulation of actin dynamics through phosphorylation of cofilin by LIM-kinase. Nature 1998; 393(6687): 805-9. [DOI:10.1038/31729]
20. Nishita M, Wang Y, Tomizawa C, Suzuki A, Niwa R, Uemura T, et al. Phosphoinositide 3-kinase-mediated activation of cofilin phosphatase Slingshot and its role for insulin-induced membrane protrusion. Journal of Biological Chemistry 2004; 279(8): 7193-8. [DOI:10.1074/jbc.M312591200]
21. Lee Y-J, Sheu T-J, Keng PC. Enhancement of radiosensitivity in H1299 cancer cells by actin-associated protein cofilin. Biochemical and biophysical research communications. Biochem Biophys Res Commun 2005; 335(2):286-91. [DOI:10.1016/j.bbrc.2005.07.073]
22. Lu L, Fu N, Luo X, et al. Overexpression of cofilin 1 in prostate cancer and the corresponding clinical implications. Oncology letters 2015; 9(6): 2757-61. [DOI:10.3892/ol.2015.3133]
23. Tammana TS, Sahasrabuddhe AA, Bajpai VK, et al. ADF/cofilin-driven actin dynamics in early events of Leishmania cell division. J Cell Sci 2010; 123(11): 1894-901. [DOI:10.1242/jcs.068494]
24. Babaei E, Montazeri V. Study of the Expression of Stem Cell Oct-4 Gene as a Diagnostic Molecular Marker in Specimens of Thyroid Papillary Carcinoma in Northwest of Iran 2016; 16(3): 292-9. (Persian)
25. Saffari Chaleshtary J, Moradi MT, Farrokhi E, et al. Study of P53 gene mutations in promoter and exons 2-4 and 9-11 in patient with gastric cancer by PCR-SSCP in Chaharmahal Va Bakhtiari province. Iran South Med J 2011; 14 (4) :220-9. (Persian)
26. Movahedi M, Afsharfard A, Moradi A, et al. Survival rate of gastric cancer in Iran. Journal of Research in Medical Sciences 2009;14(6): 367-73.
27. Castro MAA, Dal‐Pizzol F, Zdanov S, et al. CFL1 expression levels as a prognostic and drug resistance marker in nonsmall cell lung cancer. Cancer 2010; 116(15): 3645-55. [DOI:10.1002/cncr.25125]
28. Zhou J, Wang Y, Fei J, et al. Expression of cofilin 1 is positively correlated with the differentiation of human epithelial ovarian cancer. Oncology letters 2012; 4(6):1187-90. [DOI:10.3892/ol.2012.897]
29. Wang WS, Zhong HJ, Xiao DW, et al. The expression of CFL1 and N‐WASP in esophageal squamous cell carcinoma and its correlation with clinicopathological features. Diseases of the Esophagus 2010; 23(6): 512-21. [DOI:10.1111/j.1442-2050.2009.01035.x]
30. Pérez-Martínez FC, Carrión B, Lucío MI, et al. Enhanced docetaxel-mediated cytotoxicity in human prostate cancer cells through knockdown of cofilin-1 by carbon nanohorn delivered siRNA. Biomaterials 2012; 33(32): 8152-9. [DOI:10.1016/j.biomaterials.2012.07.038]
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Daryabari S, Safaralizadeh R, Hosseinpour Feizi M A, Moaddab Y, Ravanbakhsh Gavgani R. CFL1 Gene Expression in the Intestinal Samples of Gastric Adenocarcinoma in East Azarbaijan Population. Iran South Med J. 2018; 21 (1) :29-39
URL: http://ismj.bpums.ac.ir/article-1-912-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 21, Issue 1 (Iranian South Medical Journal 2018) Back to browse issues page
دانشگاه علوم پزشکی بوشهر، طب جنوب ISMJ

Iranian South Medical Journal is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License which allows users to read,
copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly

Copyright © 2022, Iranian South Medical Journal| All Rights Reserved

Persian site map - English site map - Created in 0.04 seconds with 30 queries by YEKTAWEB 4419