[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 21, Issue 5 (Iranian South Medical Journal 2018) ::
Iran South Med J 2018, 21(5): 362-373 Back to browse issues page
The Role of Estradiol in Pulmonary Hemodynamics during Ventilation with Hypoxic Gas in Female Rats Subjected to Cirrhosis
Farzaneh Ketabchi 1, Ali Sepehrinezhad2
1- Department of Physiology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran , ketabchif@sums.ac.ir
2- Department of Physiology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Abstract:   (1461 Views)
Background: Liver diseases may lead to a wide spectrum of pulmonary disorders with a high incidence in women. The aim of this study was to evaluate the effect of liver damage and ovariectomy with or without estradiol on pulmonary hemodynamics during ventilation of animals with normoxia and hypoxia gas.
Materials and Methods: Forty Sprague Dawley female rats were randomly divided into four groups of ovariectomy (OVX); ovariectomy with a daily injection of sesame oil, a solvent of estradiol (OVX+Oil); bile duct ligation with ovariectomy (CBDL+OVX) and associated with estradiol (CBDL+OVX+E2). After 28 days of the first surgery, animals were anesthetized. Tail blood samples were taken to measure liver enzymes, estradiol and NO metabolites. Animals were tracheostomized and femoral vessels were cannulated. Then, arterial pressure and right ventricular systolic pressure were recorded during mechanical ventilation with normoxic and hypoxia gas (10% oxygen).
Results: AST, AST/ALT ratio, direct and total bilirubins, and estradiol in the CBDL+OVX and CBDL+OVX+E2 groups were significantly higher than those in the OVX group, and they were higher in the CBDL+OVX+E2 group than those in the CBDL+OVX group. Ventilation of animals with hypoxia gas resulted in an increase in right ventricular systolic pressure (RVSP) only in the OVX group compared to its own base values. The plasma concentration of NO metabolites in the CBDL+OVX+E2 group was significantly higher than that in other groups.
Conclusion: Estradiol worsen the liver disorders. Furthermore, pulmonary vascular response to hypoxia gas is disrupted in liver disorders, which may be partly linked to the effect of estradiol and NO productions.
 
Keywords: RVSP, CBDL, Estradiol, NO, Hypoxia
Full-Text [PDF 831 kb]   (277 Downloads)    
Type of Study: Original | Subject: Respiratory System
Received: 2018/11/3 | Accepted: 2018/11/3 | Published: 2018/11/3
References
1. Benjaminov FS, Prentice M, Sniderman KW, et al. Portopulmonary hypertension in decompensated cirrhosis with refractory ascites. Gut 2003; 52(9): 1355–62.
2. Yang YY, Lin HC, Lee WC, et al. Portopulmonary hypertension: distinctive hemodynamic and clinical manifestations. J Gastroenterol 2001; 36(3): 181-6.
3. Krowka MJ, Mandell MS, Ramsay MA, et al. Hepatopulmonary syndrome and portopulmonary hypertension: a report of the multicenter liver transplant database. Liver Transpl 2004; 10(2): 174-82.
4. Frost AE, Badesch DB, Barst RJ, et al. The changing picture of patients with pulmonary arterial hypertension in the United States: how REVEAL differs from historic and non-US Contemporary Registries. Chest 2011; 139(1): 128-37.
5. Umar S, Iorga A, Matori H, et al. Estrogen rescues preexisting severe pulmonary hypertension in rats. Am J Respir Crit Care Med 2011; 184(6): 715-23.
6. Tofovic SP, Zhang X, Jackson EK, et al. 2-Methoxyestradiol mediates the protective effects of estradiol in monocrotaline-induced pulmonary hypertension. Vascul Pharmacol 2006; 45(6): 358-67.
7. Lahm T, Albrecht M, Fisher AJ, et al. 17 beta-Estradiol attenuates hypoxic pulmonary hypertension via estrogen receptor-mediated effects. Am J Respir Crit Care Med 2012; 185(9): 965-80.
8. Kobayashi H, Yoshida S, Sun YJ, et al. Gastric estradiol-17beta (E2) and liver ERalpha correlate with serum E2 in the cholestatic male rat. J Endocrinol 2013; 219(1): 39-49.
9. Kobayashi H, Yoshida S, Sun YJ, et al. 17 beta-Estradiol in the systemic circulation derives mainly from the parietal cells in cholestatic female rats. J Endocrinol Invest 2016; 39(4): 389-400.
10. Kobayashi H, Yoshida S, Sun YJ, et al. Gastric estrogen increases pituitary estrogen receptor alpha and prolactin mRNAs during the different pathological conditions of the liver. Endocrine 2013; 43(1): 170-83.
11. Roberts KE, Fallon MB, Krowka MJ, et al. Genetic risk factors for portopulmonary hypertension in patients with advanced liver disease. Am J Respir Crit Care Med 2009; 179(9): 835-42.
12. Assadi T, Gheybi M, Shirkani A, Movahed A, Khoddami S, Ashourinejad A, et al. Study of Prevalence and Risk Factors of Asthma and Allergic Diseases Among School Children (6-7 and 13-14 years) Based on ISAAC Protocol in Jam City, Bushehr Province in 2014. Iran South Med J 2017;19(6):972-81.
13. Earley S, Resta TC. Estradiol attenuates hypoxia-induced pulmonary endothelin-1 gene expression. Am J Physiol Lung Cell Mol Physiol 2002; 283(1): L86-93.
14. Frump AL, Goss KN, Vayl A, et al. Estradiol improves right ventricular function in rats with severe angioproliferative pulmonary hypertension: effects of endogenous and exogenous sex hormones. Am J Physiol Lung Cell Mol Physiol 2015; 308(9): L873-90.
15. Daoud FS, Reeves JT, Schaefer JW. Failure of hypoxic pulmonary vasoconstriction in patients with liver cirrhosis. J Clin Invest 1972; 51(5): 1076-80.
16. Nunes H, Lebrec D, Mazmanian M, et al. Role of nitric oxide in hepatopulmonary syndrome in cirrhotic rats. Am J Respir Crit Care Med 2001; 164(5): 879-85.
17. Marcondes FK, Bianchi FJ, Tanno AP. Determination of the estrous cycle phases of rats: some helpful considerations. Braz J Biol 2002; 62(4A): 609-14.
18. Cora MC, Kooistra L, Travlos G. Vaginal Cytology of the Laboratory Rat and Mouse: Review and Criteria for the Staging of the Estrous Cycle Using Stained Vaginal Smears. Toxicol Pathol 2015; 43(6): 776-93.
19. Gal A, Lin PC, Barger AM, et al. Vaginal fold histology reduces the variability introduced by vaginal exfoliative cytology in the classification of mouse estrous cycle stages. Toxicol Pathol 2014; 42(8): 1212-20.
20. Rodrigues DA, Da Silva AR, Serigiolle LC, et al. Constriction rate variation produced by partial ligation of the portal vein at pre-hepatic portal hypertension induced in rats. Arq Bras Cir Dig 2014; 27(4): 280-4.
21. Tag CG, Sauer-Lehnen S, Weiskirchen S, et al. Bile Duct Ligation in Mice: Induction of Inflammatory Liver Injury and Fibrosis by Obstructive Cholestasis. J Vis Exp 2015; (96): 52438.
22. Kim JL, Moon CW, Son YS, et al. Combined Effect of Bilateral Ovariectomy and Anterior Cruciate Ligament Transection With Medial Meniscectomy on the Development of Osteoarthritis Model. Ann Rehabil Med 2016; 40(4): 583–91.
23. Isaksson IM, Theodorsson A, Theodorsson E, et al. Methods for 17beta-oestradiol administration to rats. Scand J Clin Lab Invest 2011; 71(7): 583-92.
24. Miranda KM, Espey MG, Wink DA. A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide 2001; 5(1): 62-71.
25. Ketabchi F, Bajoovand S, Adlband M, et al. Right ventricular pressure elevated in one-kidney, one clip Goldblatt hypertensive rats. Clin Exp Hypertens 2017; 39(4): 344-9.
26. Chang C-C, Lee W-S, Chuang C-L, et al. Effects of raloxifene on portal hypertension and hepatic encephalopathy in cirrhotic rats. Eur J Pharmacol 2017; 802: 36-43.
27. Luo F, Ishigami M, Achiwa K, et al. Raloxifene Ameliorates Liver Fibrosis of Nonalcoholic Steatohepatitis Induced by Choline-Deficient High-Fat Diet in Ovariectomized Mice. Dig Dis Sci 2015; 60(9): 2730-9.
28. Yasuda M, Shimizu I, Shiba M, et al. Suppressive effects of estradiol on dimethylnitrosamine-induced fibrosis of the liver in rats. Hepatology 1999; 29(3): 719-27.
29. Eagon PK. Alcoholic liver injury: influence of gender and hormones. World J Gastroenterol 2010; 16(11): 1377-84.
30. Xu D, Niu W, Luo Y, et al. Endogenous estrogen attenuates hypoxia-induced pulmonary hypertension by inhibiting pulmonary arterial vasoconstriction and pulmonary arterial smooth muscle cells proliferation. Int J Med Sci 2013; 10(6): 771-81.
31. White RE, Gerrity R, Barman SA, et al. Estrogen and oxidative stress: A novel mechanism that may increase the risk for cardiovascular disease in women. Steroids 2010; 75(11): 788-93.
32. Battista S, Bar F, Mengozzi G, et al. Hyperdynamic circulation in patients with cirrhosis: direct measurement of nitric oxide levels in hepatic and portal veins. J Hepatol 1997; 26(1): 75-80.
33. Oswald‐Mammosser M, Rashid S, Boehm N, et al. Effect of the oestrogen receptor antagonist fulvestrant on the cirrhotic rat lung. Fundam Clin Pharmacol 2015; 29(3): 269-77.
34. Terao M, Takaki A, Maruyama T, et al. Serum Oxidative/anti-oxidative Stress Balance Is Dysregulated in Potentially Pulmonary Hypertensive Patients with Liver Cirrhosis: A Case Control Study. Intern Med 2015; 54(22): 2815-26.
35. Bosch J, Abraldes JG, Fernandez M, et al. Hepatic endothelial dysfunction and abnormal angiogenesis: new targets in the treatment of portal hypertension. J Hepatol 2010; 53(3): 558-67.
36. Laleman W, Omasta A, Van de Casteele M, et al. A role for asymmetric dimethylarginine in the pathophysiology of portal hypertension in rats with biliary cirrhosis. Hepatology 2005; 42(6): 1382-90.
37. Martin DS, Redetzke R, Vogel E, et al. Effect of ovariectomy on blood pressure and venous tone in female spontaneously hypertensive rats. Am J Hypertens 2008; 21(9): 983-8.
38. Lekontseva ON, Rueda-Clausen CF, Morton JS, et al. Ovariectomy in aged versus young rats augments matrix metalloproteinase-mediated vasoconstriction in mesenteric arteries. Menopause 2010; 17(3): 516-23.
39. Estrela HF, Damasio ES, Fonseca EK, et al. Differential Sympathetic Vasomotor Activation Induced by Liver Cirrhosis in Rats. PLoS One 2016; 11(4): e0152512.
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ketabchi F, Sepehrinezhad A. The Role of Estradiol in Pulmonary Hemodynamics during Ventilation with Hypoxic Gas in Female Rats Subjected to Cirrhosis. Iran South Med J. 2018; 21 (5) :362-373
URL: http://ismj.bpums.ac.ir/article-1-956-en.html


Volume 21, Issue 5 (Iranian South Medical Journal 2018) Back to browse issues page
دانشگاه علوم پزشکی بوشهر، طب جنوب ISMJ

Iranian South Medical Journal is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License which allows users to read,
copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly

Copyright © 2017, Iranian South Medical Journal| All Rights Reserved

Persian site map - English site map - Created in 0.07 seconds with 32 queries by YEKTAWEB 4137