Volume 21, Issue 2 (Iranian South Medical Journal 2018)
Iran South Med J 2018, 21(2): 147-161 |
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Darvishi M, Moradinasab M, Hosseini S J, Mohammadnia R, Aminikhoei Z, Nasri S et al . Molecular Identification of Shallow Water Zoanthids in the Coast of Bushehr- Persian Gulf. Iran South Med J 2018; 21 (2) :147-161
URL: http://ismj.bpums.ac.ir/article-1-923-en.html
URL: http://ismj.bpums.ac.ir/article-1-923-en.html
Mashallah Darvishi1 
, Maryam Moradinasab * 
2, Seyed Javad Hosseini3 , Reza Mohammadnia4 , Zahra Aminikhoei5 , Sima Nasri1 , Iraj Nabipour6
, Maryam Moradinasab * 2, Seyed Javad Hosseini3 , Reza Mohammadnia4 , Zahra Aminikhoei5 , Sima Nasri1 , Iraj Nabipour6
1- Department of Biology, School of Science, Tehran Payame NOOR University, Tehran, Iran
2- The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran ,m.moradinasab85@gmail.com
3- Department of Biotechnology, Persian Gulf Research Institute, Persian Gulf University, Busheher, Busheher, Iran
4- Bushehr Veterinary Organization, Bushehr, Iran
5- Agricultural Research Education and Extension Organization (AREEO), Iranian Fisheries Science Research Institute (IFSRI), Offshore Research Center, Chabahar, Iran
6- The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
2- The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran ,
3- Department of Biotechnology, Persian Gulf Research Institute, Persian Gulf University, Busheher, Busheher, Iran
4- Bushehr Veterinary Organization, Bushehr, Iran
5- Agricultural Research Education and Extension Organization (AREEO), Iranian Fisheries Science Research Institute (IFSRI), Offshore Research Center, Chabahar, Iran
6- The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
Abstract: (4949 Views)
Background: The order zoantharia (Cnidaria: Anthozoa) which is one of the most unexplored orders are present in the Persian Gulf. This group of cnidarians commonly found in tropical waters at various places in the world and morphological identification to the species level is extremely complicated. In the present study, molecular diversity of shallow water zoanthids from the Bushehr coasts in the Persian Gulf was investigated for the first time.
Materials and Methods: Twenty-four colonies of zoanthids with variety of shape and colors have been collected from different locations. After DNA extraction using CTAB-Phenol-Chloroform method, mitochondrial cytochrome oxidase subunit I (COI), mitochondrial 16S ribosomal DNA (mt 16S rDNA) and the nuclear internal transcribed spacer region of ribosomal DNA (ITS-rDNA) were amplified using polymerase chain reaction (PCR) technique.
Results: Based on the obtained sequences, the presence of four species-level clades: Zoanthus sansibaricus, Zoanthus pulchellus, Zoanthus sociatus and Palythoa aff. mutuki. Palythoa aff confirmed. mutuki species observed in this study had identical sequences with previously reported Palythoa aff. mutuki in the Persian Gulf. Nevertheless, in the present study two new undescribed species from genus Zoanthus (Z.sociatus and Z.pulchellus) were identifed.
Conclusion: The results of the present study indicate the efficiency of molecular markers application in comparison with morphological characteristics to identify zoaentaria, especially at the species level. Undoubtedly, more studies on zoanthids distribution patterns will increase our knowledge about the biodiversity of the Persian Gulf.
Materials and Methods: Twenty-four colonies of zoanthids with variety of shape and colors have been collected from different locations. After DNA extraction using CTAB-Phenol-Chloroform method, mitochondrial cytochrome oxidase subunit I (COI), mitochondrial 16S ribosomal DNA (mt 16S rDNA) and the nuclear internal transcribed spacer region of ribosomal DNA (ITS-rDNA) were amplified using polymerase chain reaction (PCR) technique.
Results: Based on the obtained sequences, the presence of four species-level clades: Zoanthus sansibaricus, Zoanthus pulchellus, Zoanthus sociatus and Palythoa aff. mutuki. Palythoa aff confirmed. mutuki species observed in this study had identical sequences with previously reported Palythoa aff. mutuki in the Persian Gulf. Nevertheless, in the present study two new undescribed species from genus Zoanthus (Z.sociatus and Z.pulchellus) were identifed.
Conclusion: The results of the present study indicate the efficiency of molecular markers application in comparison with morphological characteristics to identify zoaentaria, especially at the species level. Undoubtedly, more studies on zoanthids distribution patterns will increase our knowledge about the biodiversity of the Persian Gulf.
Type of Study: Original |
Subject:
General
Received: 2017/11/27 | Accepted: 2018/02/12 | Published: 2018/06/20
Received: 2017/11/27 | Accepted: 2018/02/12 | Published: 2018/06/20
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