Volume 22, Issue 2 (Iranian South Medical Journal 2019)
Iran South Med J 2019, 22(2): 77-89 |
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Sedaghat Z, Fatemikia H, Tanha K, Assadi M, Zahiri M, Seifi B et al . Evaluating the Recovery Process of Renal Ischemia/Reperfusion Injury in Rats Using Small-Animal SPECT. Iran South Med J 2019; 22 (2) :77-89
URL: http://ismj.bpums.ac.ir/article-1-1068-en.html
URL: http://ismj.bpums.ac.ir/article-1-1068-en.html
Zahra Sedaghat * 
1, Hossein Fatemikia2 , Kaveh Tanha3 , Majid Assadi4 , Mariya Zahiri5 , Behjat Seifi6 , Khalil Pourkhalili7
1, Hossein Fatemikia2 , Kaveh Tanha3 , Majid Assadi4 , Mariya Zahiri5 , Behjat Seifi6 , Khalil Pourkhalili7
1- Physiology Department, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran , z.sedaghat@bpums.ac.ir
2- Physiology Department, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
Physiology Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
3- Medical physics and biomedical engineering department, Tehran University of Medical Sciences, Tehran, Iran
The Persian Gulf Nuclear Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
4- The Persian Gulf Nuclear Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
5- Anatomy Department, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
6- Physiology Department, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
7- Physiology Department, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
2- Physiology Department, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
Physiology Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
3- Medical physics and biomedical engineering department, Tehran University of Medical Sciences, Tehran, Iran
The Persian Gulf Nuclear Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
4- The Persian Gulf Nuclear Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
5- Anatomy Department, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
6- Physiology Department, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
7- Physiology Department, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
Abstract: (4838 Views)
Background: Renal injuries associated with ischemia/reperfusion are a prevalent clinical phenomenon that can cause the emergence of progressive kidney diseases, eventually leading to chronic kidney injuries. The present study was conducted to evaluate the results obtained from non-invasive imaging using small-animal SPECT and investigate the recovery process in an animal model of renal ischemia/reperfusion.
Materials and Methods: Male Wistar rats were used to establish a unilateral model of renal ischemia/ reperfusion injury. After occluding the left renal pedicle for 120 minutes, the animals were investigated in terms of reperfusion at 24 hours, one week and three weeks. At each time point, the intravascular injection of 99mTc-DMSA as well as scanning with the SPECT machine were conducted. Blood sampling and renal biopsy were also performed.
Results: After 24 hours, the accumulated activity levels were significantly lower in the ischemic kidney compared to in the contralateral intact kidney. Severe renal histologic changes were also observed. After one and three weeks, the radiopharmaceutical uptake increased in the ischemic compared to both the contralateral kidney and the time point of 24 hours, and the absorbed activity was divided between the two kidneys in a more balanced fashion, which is quite consistent with the histologic results.
Conclusion: The present findings suggest that non-invasive imaging with a small-animal SPECT system using 99mTc-DMSA provides researchers with an appropriate tool in rodent models of renal ischemic damage for evaluating the long-term follow-up of kidney recovery. The obtained results also appear to be thoroughly consistent with invasive histological studies.
Materials and Methods: Male Wistar rats were used to establish a unilateral model of renal ischemia/ reperfusion injury. After occluding the left renal pedicle for 120 minutes, the animals were investigated in terms of reperfusion at 24 hours, one week and three weeks. At each time point, the intravascular injection of 99mTc-DMSA as well as scanning with the SPECT machine were conducted. Blood sampling and renal biopsy were also performed.
Results: After 24 hours, the accumulated activity levels were significantly lower in the ischemic kidney compared to in the contralateral intact kidney. Severe renal histologic changes were also observed. After one and three weeks, the radiopharmaceutical uptake increased in the ischemic compared to both the contralateral kidney and the time point of 24 hours, and the absorbed activity was divided between the two kidneys in a more balanced fashion, which is quite consistent with the histologic results.
Conclusion: The present findings suggest that non-invasive imaging with a small-animal SPECT system using 99mTc-DMSA provides researchers with an appropriate tool in rodent models of renal ischemic damage for evaluating the long-term follow-up of kidney recovery. The obtained results also appear to be thoroughly consistent with invasive histological studies.
Type of Study: Original |
Subject:
Physiology
Received: 2017/07/12 | Accepted: 2019/02/18 | Published: 2019/05/18
Received: 2017/07/12 | Accepted: 2019/02/18 | Published: 2019/05/18
References
1. Bellomo R, Kellum JA, Ronco C. Acute Kidney Injury. The Lancet 2012; 380(9843): 756-66. [DOI:10.1016/S0140-6736(11)61454-2]
2. Chatauret N, Badet L, Barrou B, et al. Ischemia-reperfusion: From Cell Biology to Acute Kidney Injury. Prog Uro 2014; 24: S4-S12. [DOI:10.1016/S1166-7087(14)70057-0]
3. Seifi B, Kadkhodaee M, Bakhshi E, et al. Angiotensin II of Paraventricular Nucleus is Exaggerated the Renal Ischemia-reperfusion Injury. Tehran Univ Med J 2014; 72(2): 96-105.
4. Sedaghat Z, Kadkhodaee M, Seifi B, et al. Hind Limb Perconditioning Renoprotection by Modulation of Inflammatory Cytokines After Renal Ischemia/reperfusion. Ren Fail 2016; 38(5): 655-62. [DOI:10.3109/0886022X.2016.1155387]
5. Mahmoudi A, Kadkhodaee M, Golab F, et al. Evaluation of the Gender Difference in the Protective Effects of Ischemic Postconditioning on Ischemia-reperfusion-induced Acute Kidney Injury in Rats. Tehran Univ Med J 2013; 71(8): 485-92.
6. Seifi B, Kadkhodaee M, Bakhshi E, et al. Angiotensin II in Paraventricular Nucleus Contributes to Sympathoexcitation in Renal Ischemia-reperfusion Injury by AT1 Receptor and Oxidative Stress. J Surg Res 2015; 193(1): 361-7. [DOI:10.1016/j.jss.2014.06.042]
7. Fatemi Tabatabaei S R, Askaripour M, Hosseini F, et al. The Effect of Aqueous Purslane (Portulaca Oleracea) Extract on Renal Ischemia/Reperfusion Injury in Rat. Iran South Med J 2015; 18(3): 486-96. (Persian)
8. Venkatachalam MA, Griffin KA, Lan R, et al. Acute Kidney Injury: A Springboard for Progression in Chronic Kidney Disease. Am J Physiol Renal Physiol 2010; 298(5): F1078-94. [DOI:10.1152/ajprenal.00017.2010]
9. Singh AP, Muthuraman A, Jaggi AS, et al. Animal Models of Acute Renal Failure. Pharmacol Rep 2012; 64(1): 31-44. [DOI:10.1016/S1734-1140(12)70728-4]
10. Le Clef N, Verhulst A, D'Haese PC, et al. Unilateral Renal Ischemia-reperfusion As a Robust Model for Acute to Chronic Kidney Injury in Mice. PloS one 2016; 11(3): e0152153. [DOI:10.1371/journal.pone.0152153]
11. Ascon M, Ascon DB, Liu M, et al. Renal Ischemia-reperfusion Leads to Long Term Infiltration of Activated and Effector-memory T Lymphocytes. Kidney Int 2009; 75(5): 526-35. [DOI:10.1038/ki.2008.602]
12. Ardela DE, Miguel MB, Gutiérrez DJ, et al. [Comparative Study of Differential Renal Function by DMSA and MAG-3 in Congenital Unilateral Uropathies]. Cir Pediatr 2002; 15(3): 118-21.
13. Rudin M, Weissleder R. Molecular Imaging in Drug Discovery and Development. Nat Rev Drug Discov 2003; 2(2): 123-31. [DOI:10.1038/nrd1007]
14. Tai Y-C, Laforest R. Instrumentation Aspects of Animal PET. Annu Rev Biomed Eng 2005; 7: 255-85. [DOI:10.1146/annurev.bioeng.6.040803.140021]
15. Sheridan AM, Bonventre JV. Cell Biology and Molecular Mechanisms of Injury in Ischemic Acute Ren Fail. Curr Opin Nephrol Hypertens 2000; 9(4): 427-34. [DOI:10.1097/00041552-200007000-00015]
16. Pashazadeh A, Tanha K, Jafarian-Dehkordi F, et al. Experimental Evaluation of the Performance of HiReSPECT Scanner: A High-resolution SPECT System for small Animal Imaging. Fron Biomed Tech 2015; 1(3): 222-7.
17. Moji V, Zeratkar N, Farahani MH, et al. Performance Evaluation of a Newly Developed High-resolution, Dual-head Animal SPECT System Based on the NEMA NU1-2007 Standard. J Appl Clin Med Phys 2014; 15(6): 4936. [DOI:10.1120/jacmp.v15i6.4936]
18. Phelps ME. Positron Emission Tomography Provides Molecular Imaging of Biological processes. Proc Natl Acad Sci 2000; 97(16): 9226-33. [DOI:10.1073/pnas.97.16.9226]
19. Dhull RS, Joshi A, Saha A. Nuclear Imaging in Pediatric Kidney Diseases. Indian Pediatr 2018; 55(7): 591-7. [DOI:10.1007/s13312-018-1303-7]
20. Wolf W. Introduction and Overview of Noninvasive Drug Monitoring. Adv Drug Deliv Rev 2000; 41(1):1-5. [DOI:10.1016/S0169-409X(99)00052-6]
21. Abedi SM, Mohammadjafari H, Hosseinimehr SJ, et al. Imaging of Renal Cortex in Nuclear Medicine. Clin Excel 2014; 2(1): 50-69
22. Taylor A. Quantitation of Renal Function with Static Imaging Agents. Semin Nucl Med 1982; 12: 330-44. [DOI:10.1016/S0001-2998(82)80014-7]
23. Weyer K, Nielsen R, Petersen SV, et al. Renal Uptake of 99mTc-Dimercaptosuccinic Acid Is Dependent on Normal Proximal Tubule Receptor-Mediated Endocytosis. J Nucl Med 2013;54(1):159-65. [DOI:10.2967/jnumed.112.110528]
24. Piepsz A, Blaufox M, Gordon I, et al. Consensus On Renal Cortical Scintigraphy in Children with Urinary Tract Infection. Semin Nucl Med 1999; 29: 160-74. [DOI:10.1016/S0001-2998(99)80006-3]
25. Majd M, Nussbaum Blask AR, Markle BM, et al. Acute Pyelonephritis: Comparison of Diagnosis with 99mTc-DMSA SPECT, Spiral CT, MR Imaging, and Power Doppler Us in an Experimental Pig Model. Radiology 2001; 218(1): 101-8. [DOI:10.1148/radiology.218.1.r01ja37101]
26. Fatemikia H, Seyedabadi M, Karimi Z, et al. Comparison of 99mTc-DMSA Renal Scintigraphy with Biochemical and Histopathological Findings in Animal Models of Acute Kidney Injury. Mol Cell Biochem 2017; 434(1-2): 163-9. [DOI:10.1007/s11010-017-3046-5]
27. Sancak EB, Tan YZ, Turkon H, et al. Attenuation of Partial Unilateral Ureteral Obstruction-Induced Renal Damage with Hyperbaric Oxygen Therapy in a Rat Model. Int braz j urol 2017; 43(5): 946-56. [DOI:10.1590/s1677-5538.ibju.2016.0565]
28. Coca SG, Yusuf B, Shlipak MG, et al. Longterm Risk of Mortality and Other Adverse Outcomes After Acute Kidney Injury: A Systematic Review and Meta-analysis. Am J Kidney Dis 2009; 53(6): 961-73. [DOI:10.1053/j.ajkd.2008.11.034]
29. Chawla LS, Kimmel PL. Acute Kidney Injury and Chronic Kidney Disease: An Integrated Clinical Syndrome. Kidney Int 2012; 82(5): 516-24 [DOI:10.1038/ki.2012.208]
30. Karimi Z, Ketabchi F, Alebrahimdehkordi N, et al. Renal Ischemia/reperfusion Against Nephrectomy for Induction of Acute Lung Injury in Rats. Ren Fail 2016; 38(9): 1503-15. [DOI:10.1080/0886022X.2016.1214149]
31. Fatemikia H, Ketabchi F, Karimi Z, et al. Distant Effects of Unilateral Renal Ischemia/reperfusion on Contralateral Kidney But Not Lung in Rats: The Roles of ROS and iNOS. Can J Physiol Pharmacol 2016; 94(5): 477-87. [DOI:10.1139/cjpp-2015-0285]
32. Rabb H, Wang Z, Nemoto T, et al. Acute Renal Failure Leads to Dysregulation of Lung Salt and Water Channels. Kidney Int 2003; 63(2): 600-6. [DOI:10.1046/j.1523-1755.2003.00753.x]
33. O'Donnell MP, Burne M, Daniels F, et al. Utility and Limitations of Serum Creatinine As a Measure of Renal Function in Experimental Renal Ischemia-reperfusion Injury. Transplantation 2002; 73(11): 1841-4. [DOI:10.1097/00007890-200206150-00025]
34. Ketabchi F, Sepehrinezhad A, The Role of Estradiol in Pulmonary Hemodynamics during Ventilation with Hypoxic Gas in Female Rats Subjected to Cirrhosis. Iran South Med J 2018; 21(5):362-73. (Persian)
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