Volume 17, Issue 4 (Iranian South Medical Journal 2014)                   Iran South Med J 2014, 17(4): 506-516 | Back to browse issues page

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Khamisipour G R, Pourfathollah A A, Soleimani M, Fourouzandeh Moghaddam M, Norouzinia M, Kavyani S, et al . Generation of hematopoietic lineage cells from embryonic like cells. Iran South Med J 2014; 17 (4) :506-516
URL: http://ismj.bpums.ac.ir/article-1-566-en.html
1- Department of laboratory sciences, School of Para medicine, Bushehr University of Medical Sciences, Bushehr, IRAN
2- Department of Immunology, School of Medical Sciences, Tarbiat Modares University, Tehran, IRAN , pourfa@modares.ac.ir
3- Department of Hematology, School of Medical Sciences, Tarbiat Modares University, Tehran, IRAN
4- Department of Biotechnology, School of Medical Sciences, Tarbiat Modares University, Tehran, IRAN
5- Department of Medical Genetic, School of Medical Sciences, Tarbiat Modares University, Tehran, IRAN
6- Department of Oncology, School of Medicine, Tehran University of Medical Sciences, Tehran, IRAN
7- The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Research Center, Bushehr University of Medical Sceinecs, Bushehr, IRAN
8- Department of Surgery, School of Medicine, Bushehr University of Medical Sciences, Bushehr, IRAN
Abstract:   (5296 Views)

Background: Epigenetic reprogramming of somatic cells into embryonic stem cells has attracted much attention, because of the potential for stem cell transplantation and compatibility with recipient. However, the therapeutic application of either nuclear transfer or nuclear fusion of somatic cell has been hindered by technical complications as well as ethical objections. Recently, a new method is reported whereby ectopic expression of embryonic specific transcription factors was shown to induce fibroblasts to become embryonic like SCs (induced pluripotent stem cells). A major limitation of this method is the use of potentially harmful genome integrating viruses such as reto- or lentivirus. The main aim of this investigation was generation of human hematopoietic stem cells from induced fibroblasts by safe adenovectors carrying embryonically active genes. Material and Methods: Isolated fibroblasts from foreskin were expanded and recombinant adenoviruses carrying human Sox2, Oct4, Klf4, cMyc genes were added to culture. After formation of embryonic like colonies and cell expansion, they were transferred to embryonic media without bFGF, and embryoid bodies were cultured on stromal and non-stromal differentiation media for 14 days. Results: Expression of CD34 gene and antigenic markers, CD34, CD38 & CD133 in stromal culture showed significant difference with non-differentiation and non-stromal media. Conclusion: These findings show high hematopoietic differentiation rate of Adeno-iPS cells in stromal culture and no need to use growth factors. While, there was no difference between non-differentiation and non-stromal media.

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Type of Study: Original | Subject: General
Received: 2011/11/8 | Accepted: 2012/04/11 | Published: 2014/08/10

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