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:: Volume 19, Issue 6 (Iranian South Medical Journal 2017) ::
Iran South Med J 2017, 19(6): 931-939 Back to browse issues page
Association of HSP70-2 Gene 1267A/G Polymorphism With Cataract Incidence Among Guilan Population
Zivar Salehi 1, Zahra Gholaminia2, Mohammad Reza Panjtanpanah3
1- Department of Biology, School of Sciences, University of Guilan, Rasht, Iran , geneticzs@yahoo.co.uk
2- Department of Biology, School of Sciences, University of Guilan, Rasht, Iran
3- Department of Ophthalmology, School of medicine, Guilan University of Medical Sciences, Rasht, Iran
Abstract:   (6393 Views)

Background: Cataract is a visible opacity of the eye lens and it is the main reason of reversible blindness in the world. Oxidative stress is known as a major factor in cataract formation HSP70-2 protein is a molecular chaperone which is essential for cell survival in stress conditions. HSP70-2 gene is located in the human leukocyte antigen class ΙΙΙ region. This gene encodes an inducible protein. One of the common polymorphism of HSP70-2 is 1267A/G which is located in coding region. The aim of this study was to analysis of 1267A/G polymorphism of hsp70-2 gene in cataract patients.

Material and Methods: This case-control study included 118 cataract patients and 122 healthy people as a control groups. Genomic DNA was extracted from peripheral blood leukocytes and genotyping determination was performed by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Statistical analysis was performed by using the MedCalc software (Version 12.1).

Results: The frequency of G allele was significantly higher in patients than the controls, (0.36 and 0.24, respectively). A higher frequency of the GG genotype of the HSP70-2 1267A/G polymorphism was found in the patients compared with controls (21.19% and 8.20%, respectively). The patients carrying the GG genotype were 3.2-fold at a higher risk of cataract compared with AA genotype (P=0.005).

Conclusion: The finding of this study suggested that the HSP70-2 1267A/G may affect the susceptibility to cataract in the studied population. Anyway the randomized multicenter studies with greater sample size still need to confirmed our results.

Keywords: Cataract, gene polymorphism, HSP70-2 gene, oxidative stress
Full-Text [PDF 717 kb]   (2033 Downloads)    
Subject: Biochemistry. Cell Biology and Genetics
Received: 2015/08/26 | Accepted: 2015/12/7 | Published: 2016/12/27
1. Resnikoff S, Pascolini D, Etya’ale D, et al. Global data on visual impairment in the year 2002. Bull World Health Organ 2004; 82(11): 844-51. [PubMed] [Google Scholar]
2. Sibug ME, Datiles MB, Kashima K, et al. Specular microscopy studies on the corneal endothelium after cessation of contact lens wear. Cornea 1991; 10(5): 395-401. [PubMed] [Google Scholar]
3. Chang JR, Koo E, Agrón E, et al. Risk factors associated with incident cataracts and cataract surgery in the age related eye disease study (AREDS): AREDS report number 32. Ophthalmology 2011; 118(11): 2113-9. [PubMed] [Google Scholar]
4. Hashemi H, Alipour F, Mehravaran S, et al. Five year cataract surgical rate in Iran. Optomet Vis Sci 2009; 86(7): 890-4. [PubMed] [Google Scholar]
5. Zhang Y, Zhang L, Sun D, et al. Genetic polymorphisms of superoxide dismutases, catalase, and glutathione peroxidase in age-related cataract. Mol Vis 2011; 17: 2325-32. [PubMed] [Google Scholar]
6. Spector A. Oxidative stress-induced cataract: mechanism of action. FASEB J 1995; 9(12): 1173-82. [PubMed] [Google Scholar]
7. Gombos T, Förhécz Z, Pozsonyi Z, et al. Interaction of serum 70-kDa heat shock protein levels and HspA1B (+1267) gene polymorphism with disease severity in patients with chronic heart failure. Cell Stress Chaperones 2008; 13(2): 199-206. [PubMed] [Google Scholar]
8. Liu J, Cheng J, Peng J, et al. Effects of polymorphisms of heat shock protein 70 gene on ischemic stroke, and interaction with smoking in China. Clin Chim Acta 2007; 384(1-2): 64-8. [PubMed] [Google Scholar]
9. Pollreisz A, Schmidt-Erfurth U. Diabetic cataract-pathogenesis, epidemiology and treatment. J Ophthalmol 2010; 2010: 608751. [PubMed] [Google Scholar]
10. Olinski R, Siomek A, Rozalski R, et al. Oxidative damage to DNA and antioxidant status in aging and age-related diseases. Acta Biochim Pol 2007; 54(1): 11-26. [PubMed] [Google Scholar]
11. Li WC, Kuszak JR, Dunn K, et al. Lens epithelial cell apoptosis appears to be a common cellular basis for non-congenital cataract development in humans and animals. J Cell Biol 1995; 130(1): 169-81. [PubMed] [Google Scholar]
12. Wang Y, King JA. Cataract as a Protein‐Aggregation Disease. Protein Misfolding Diseases: Current and Emerging Principles and Therapies 2010: 487-515. [Google Scholar]
13. Lixia S, Yao K, Kaijun W, et al. Effects of 1.8 GHz radiofrequency field on DNA damage and expression of heat shock protein 70 in human lens epithelial cells. Mutat Res 2006; 602(1-2): 135-42. [PubMed] [Google Scholar]
14. Mayer MP, Bukau B. Hsp70 chaperones: cellular functions and molecular mechanism. Cell Mol life Sci 2005; 62(6): 670-84. [PubMed] [Google Scholar]
15. Milner CM, Campbell RD. Polymorphic analysis of the three MHC-linked HSP70 genes. Immunogenetics 1992; 36(6): 357-62. [PubMed] [Google Scholar]
16. Bruner SD, Norman DP, Verdine GL. Structural basis for recognition and repair of the endogenous mutagen 8-oxoguanine in DNA. Nature 2000; 403(6772): 859-66. [PubMed] [Google Scholar]
17. Ferrer-Ferrer M, Malespín-Bendaña W, Ramírez V, et al. Polymorphisms in genes coding for HSP-70 are associated with gastric cancer and duodenal ulcer in a population at high risk of gastric cancer in Costa Rica. Arch Med Res 2013; 44(6): 467-74. [PubMed] [Google Scholar]
18. Szondy K, Rusai K, Szabó AJ, et al. Tumor cell expression of heat shock protein (HSP) 72 is influenced by HSP72 [HSPA1B A(1267)G] polymorphism and predicts survival in small Cell lung cancer (SCLC) patients. Cancer Invest 2012; 30(4): 317-22. [PubMed] [Google Scholar]
19. Fraile A, Nieto A, Mataran L, et al. HSP70 gene polymorphisms in ankylosing spondylitis. Tissue Antigens 1998; 51(4 Pt 1): 382-5. [PubMed] [Google Scholar]
20. Giacconi R, Caruso C, Lio D, et al. 1267 HSP70-2 polymorphism as a risk factor of statins on adhesion molecule expression in endothelial cells for carotid plaque rupture and cerebral ischaemia in old type 2 diabetes-atherosclerotic patients. Mech Ageing Dev 2005; 126: 866-73. [Google Scholar]
21. Thylefors B, Négrel AD, Pararajasegaram R, et al. Global data on blindness. Bull World Health Organ 1995; 73(1): 115-21. [PubMed] [Google Scholar]
22. Zhao R, Yang Y, He X, et al. An autosomal dominant cataract locus mapped to 19q13-qter in a Chinese family. Mol Vis 2011; 17: 265-9. [PubMed] [Google Scholar]
23. Charakidas A, Kalogeraki A, Tsilimbaris M, et al. Lens epithelial apoptosis and cell proliferation in human age-related cortical cataract. Eur J Ophthalmol 2005; 15(2): 213-20. [PubMed] [Google Scholar]
24. Weinreb O, Dovrat A, Dunia I, et al. UV-A-related alterations of young and adult lens water-insoluble alpha-crystallin, plasma membranous and cytoskeletal proteins. Eur J Biochem 2001; 268(3): 536-43. [PubMed] [Google Scholar]
25. Brocchieri L, Conway de Macrio E, Macario AJ. Hsp70 genes in the human genome: Conservation and differentiation patterns predict a wide array of overlapping and specialized functions. BMC Evol Biol 2008; 8: 19. [PubMed] [Google Scholar]
26. Hunt C, Morimoto RI. Conserved features of eukaryotic hsp70 genes revealed by comparison with the nucleotide sequence of human hsp70. Proc Natl Acad Sci USA1985; 82(19): 6455-59. [PubMed] [Google Scholar]
27. Fekete A, Treszl A, Toth-Heyn P, et al. Association between heat shock protein 72 gene polymorphism and acute renal failure in premature neonates. Pediatr Res 2003; 54(4): 452-5. [PubMed] [Google Scholar]
28. Bagchi M, Katar M, Maisel H. Heat shock proteins of adult and embryonic human ocular lenses. J Cell Biochem 2002; 84(2): 278-84. [PubMed] [Google Scholar]
29. Zhang Y, Gong J, Zhang L, et al. Genetic polymorphisms of HSP70 in age-related cataract. Cell Stress Chaperones 2013; 18(6): 703-9. [PubMed] [Google Scholar]
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Salehi Z, Gholaminia Z, Panjtanpanah M R. Association of HSP70-2 Gene 1267A/G Polymorphism With Cataract Incidence Among Guilan Population. Iran South Med J. 2017; 19 (6) :931-939
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