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:: Volume 19, Issue 3 (Iranian South Medical Journal 2016) ::
Iran South Med J 2016, 19(3): 334-341 Back to browse issues page
Study of Polymorphism Pro198Leu of GPX-1 gene as a risk factor in patients with colorectal cancer
Zivar Salehi 1, Fatemeh Hosseini2, Farzam Ajamian2, Hamid Saeidi Saedi3
1- Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran , geneticzs@yahoo.co.uk
2- Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran
3- Department of Radiotherapy and Oncology, Faculty of Medicine, University of Medical Sciences, Rasht, Iran
Abstract:   (4790 Views)

Background: Oxidative stress is a condition in which the balance is disrupted between reactive oxygen species (ROS) and antioxidant. Glutathione peroxidase 1(GPX-1), as one of the antioxidant enzyme remove the ROS in a continuous process. One of the functional polymorphism of GPX1 gene is Pro198Leu polymorphism. The aim of this study was to study the association between GPX-1 Pro198Leu polymorphism with the risk of colorectal cancer.

Materials & Methods: in this case-control study, 130 patients with colorectal cancer were compared with 170 healthy subjects. Genomic DNA was extracted from peripheral blood leukocytes. Determination of DNA genotyping in GPX-1 Pro198Leu polymorphism were performed by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The χ2-test was used for statistical analyses.

Results: The GPX1 genotype frequencies were 64% for CC, 24% for CT and 12% for TT in control group, and 60% for CC, 30% for CT and 12% for TT among the patients. The C allele frequency in cases and controls including 0.75 and 0.76 and T allele frequency was including 0.25 and 0.24. Based on these data, there was no significant differences in the GPX1 Pro198Leu genotypes and allele frequencies between cases and controls (P=0.2).

Conclusion: The result of this study suggested that GPX-1 Pro198Leu polymorphism could not be a risk factor for colorectal cancer. However, studies in larger populations are needed in order to confirm the results.

Keywords: Colorectal cancer, GPX-1, ROS, gene polymorphism
Full-Text [PDF 405 kb]   (1387 Downloads)    
Type of Study: Original | Subject: Biochemistry. Cell Biology and Genetics
Received: 2015/05/17 | Accepted: 2015/07/23 | Published: 2016/07/17
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Salehi Z, Hosseini F, Ajamian F, Saeidi Saedi H. Study of Polymorphism Pro198Leu of GPX-1 gene as a risk factor in patients with colorectal cancer. Iran South Med J. 2016; 19 (3) :334-341
URL: http://ismj.bpums.ac.ir/article-1-797-en.html

Volume 19, Issue 3 (Iranian South Medical Journal 2016) Back to browse issues page
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