Volume 23, Issue 3 (Iranian South Medical Journal 2020)                   Iran South Med J 2020, 23(3): 195-204 | Back to browse issues page

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Ghaderi M, Khamisipour G, Obeidi N, Tahmasebi R, Mohammadi S A, Rastgoo R et al . Pathway Analysis of miRNA-1 and Its Expres-sion Evaluation in Donor’s Serum from HIV-Positive Individuals vs Unaffected Controls. Iran South Med J 2020; 23 (3) :195-204
URL: http://ismj.bpums.ac.ir/article-1-1303-en.html
1- Department of Hematology, School of Allied Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
2- Department of Hematology, School of Allied Medicine, Bushehr University of Medical Sciences, Bushehr, Iran , ghr.khamisi@gmail.com
3- Department of Biostatistics, School of Public Health, Bushehr University of Medical Sciences, Bushehr ,Iran
4- Department of Cellular and Molecular Biology, School of Basic Sciences, University of Isfahan, Isfahan, Iran
Abstract:   (2970 Views)
Background MicroRNAs (miRNAs) are non-coding RNA molecules (19-24 nucleotides) that play a major role in a wide range of biological processes through post-transcriptional regulation of gene expression. Differential expression of miRNAs has been reported in various infectious diseases such as HIV infection. The characterization of miRNA expression profiles, especially in mammalian biofluids, which are affected by intracellular processes of different parts of the body, provides a considerable insight into pathophysiological alterations associated with host-virus interactions. Therefore, based on miR-1 bioinformatics analysis in the context of potential affected signaling pathways in HIV infection, the present study aimed to profile the expression of miR-1 in donor’s serum from HIV-positive individuals vs unaffected controls in Bushehr Blood Transfusion Center.
Materials and Methods: The enrichment analysis on predicted target genes for miR-1 was performed based on miRNA-related bioinformatics tools to achieve possible regulated pathways by this miRNA. Given the pathways affected by HIV infection and the involvement of miR-1 target genes in these signaling pathways, the miR-1 expression was evaluated in serum samples of  20 treated HIV-positive individuals, 10 patients with de novo HIV infection diagnosis, and 40 healthy subjects using qRT-PCR. Data were analyzed by SPSS software version 22.
Results: The enrichment analysis for identified target genes of miR-1 revealed that target genes were significantly enriched (FDR <0.05) in the four pathways of thyroid hormone, cancer pathways, endocytosis, and phosphatidylinositol signaling system. In addition, a significant increase was observed in miR-1 expression level  (p-value <0.05) in de novo HIV infected patients compared with healthy subjects. A significant decrease was observed in miR-1 expression level (p-value <0.0001) in treated HIV-positive individuals compared with de novo HIV infected patients, as well.
Conclusion: Bioinformatics studies indicate that the predicted target genes for miR-1 belong to the pathways related to thyroid hormone, cancer, endocytosis, and phosphatidylinositol signaling system, which are impaired pathways in HIV infection. In addition, significantly altered expression in miR-1 in the serum of de novo HIV infected patients vs healthy subjects and treated HIV-positive individuals vs de novo HIV infected patients represents remarkable effects of the process of HIV infection and pathogenesis on miR-1 expression. Hence, the findings of this study indicate the potential use of miR-1 to better understand HIV pathogenesis, and subsequent therapeutic interventions by targeting miRNA.
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Type of Study: Original | Subject: Hemic and Lymphatic Systems
Received: 2018/06/30 | Accepted: 2020/02/5 | Published: 2020/07/27

1. Grez M, Dietrich U, Balfe P, et al. Genetic Analysis Of Human Immunodeficiency Virus Type 1 And 2 (HIV-1 And HIV-2) Mixed Infections In India Reveals A Recent Spread Of HIV-1 And HIV-2 From A Single Ancestor For Each Of These Viruses. J Virol 1994; 68(4): 2161-8. [DOI:10.1128/JVI.68.4.2161-2168.1994]
2. Nam JG, Kim GJ, Baek JY, et al. Molecular Investigation Of Human Immunodeficiency Virus Type 2 Subtype A Cases In South Korea. J Clin Microbiol 2006; 44(4): 1543-6. [DOI:10.1128/JCM.44.4.1543-1546.2006]
3. Kanki PJ, Travers KU, Marlink R, et al. Slower Heterosexual Spread Of HIV-2 Than HIV-1. Lancet 1994; 343(8903): 943-6. [DOI:10.1016/S0140-6736(94)90065-5]
4. Verma P, Pandey RK, Prajapati P, et al. Circulating Micrornas: Potential And Emerging Biomarkers For Diagnosis Of Human Infectious Diseases. Front Microbiol 2016; 7: 1274. [DOI:10.3389/fmicb.2016.01274]
5. Balasubramaniam M, Pandhare J, Dash C. Are Micrornas Important Players In HIV-1 Infection? An Update. Viruses 2018; 10(3): e110. [DOI:10.3390/v10030110]
6. Ahmadi K, Soleimani M, Kaviani S, et al. Effect Of Overexpression Of Mir-29b1 On Induction Of Apoptosis In Leukemic HL60 Cell Line. Iran South Med J 2014; 17(3): 243-53. (Persian)
7. Munshi SU, Panda H, Holla P, et al. Microrna150 Is A Potential Biomarker Of HIV/AIDS Disease Progression And Therapy. Plos One 2014; 9(5): E95920. [DOI:10.1371/journal.pone.0095920]
8. Zhou Y, Sun L, Wang X, et al. Short Communication: HIV-1 Infection Suppresses Circulating Viral Restriction Micrornas. AIDS Res Hum Retroviruses 2016; 32(4): 386-9. [DOI:10.1089/aid.2015.0253]
9. Kraj B. Incorporation Of Molecular Diagnostics Into Medical Laboratory Science Curriculum: Clinical Facilities Expectations. An Asynchronous, Iterative, Online Delphi Study [dissertation]. Virginia: Virginia Commonwealth Univ., 2015.
10. Akhtar MM, Micolucci L, Islam MS, et al. Bioinformatic Tools For Microrna Dissection. Nucleic Acids Res 2016; 44(1): 24-44. [DOI:10.1093/nar/gkv1221]
11. François F, Klotman ME. Phosphatidylinositol 3-Kinase Regulates Human Immunodeficiency Virus Type 1 Replication Following Viral Entry In Primary CD4+ T Lymphocytes And Macrophages. J Virol 2003; 77(4): 2539-49. [DOI:10.1128/JVI.77.4.2539-2549.2003]
12. Marsh M, Pelchen‐Matthews A. Endocytosis In Viral Replication. Traffic 2000; 1(7): 525-32. [DOI:10.1034/j.1600-0854.2000.010701.x]
13. Elfaki MG. Immunosuppression Induced By HIV Infection. Biol Med 2014; 6(3): 1. [DOI:10.4172/0974-8369.1000e111]
14. Noureldeen AF, Qusti SY, Khoja GM. Thyroid Function In Newly Diagnosed HIVInfected Patients. Toxicol Ind Health 2014; 30(10): 919-25. [DOI:10.1177/0748233712466133]

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