Volume 26, Issue 2 (Iranian South Medical Journal 2023)
Iran South Med J 2023, 26(2): 77-91 |
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Akbari Z, Ansari I, Karimi Z, Zendeboodi S, Tanha K, Asadi M et al . Repeated Daily Normobaric Hyperoxia: A Non-Pharmacological Strategy Against Gentamicin-Induced Nephrotoxicity. Iran South Med J 2023; 26 (2) :77-91
URL: http://ismj.bpums.ac.ir/article-1-1814-en.html
URL: http://ismj.bpums.ac.ir/article-1-1814-en.html
Zahra Akbari1 
, Iman Ansari2 , Zienab Karimi3 , Sodabeh Zendeboodi4 , Kaveh Tanha5 , Majid Asadi6 , Khalil Pourkhalili * 7
, Iman Ansari2 , Zienab Karimi3 , Sodabeh Zendeboodi4 , Kaveh Tanha5 , Majid Asadi6 , Khalil Pourkhalili * 7
1- Department of Physiology, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
2- Student Research Committee, Bushehr University of Medical Sciences, Bushehr, Iran
3- Nephro-Urology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
4- Department of Internal Medicine, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
5- Medical Physics and Biomedical Engineering Department, Tehran University of Medical Sciences, Tehran, Iran
6- The Persian Gulf Nuclear Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
7- Department of Physiology, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran , kh_pourkhalili@yahoo.com
2- Student Research Committee, Bushehr University of Medical Sciences, Bushehr, Iran
3- Nephro-Urology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
4- Department of Internal Medicine, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
5- Medical Physics and Biomedical Engineering Department, Tehran University of Medical Sciences, Tehran, Iran
6- The Persian Gulf Nuclear Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
7- Department of Physiology, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran , kh_pourkhalili@yahoo.com
Abstract: (629 Views)
Background: Gentamicin (GM) induced nephrotoxicity is one of the most common causes of acute kidney injury and limits its administration. Evidence suggests that pre-treatment with oxygen increases the activity of antioxidant enzymes and protects tissues against different kinds of damage. In this study, the effect of daily normobaric heperoxia pre-treatment on alleviating gentamicin induced nephrotoxicity was investigated.
Materials and Methods: Twenty-eight male Wistar rats were randomly divided into four groups (n=7): Control (room air); Gentamicin (100 mg/kg, IP, for 9 days); Hyperoxia60 (60 min daily pretreatment with 95% oxygen and then 100 mg/kg gentamicin, IP, for 9 days) and Hyperoxia180 (180 min daily pretreatment with 95% oxygen and then 100 mg/kg gentamicin, IP, for 9 days). Then 24 h urine was collected and on day 10, the rats were sacrificed for serum, urine and renal tissue sampling.
Results: Results showed that hyperoxia significantly enhanced renal antioxidative capacity and decreased serum creatinine and renal failure index. Histological examination and SPECT scan also showed that tissue damage in the hyperoxia groups was lower compared to the gentamicin group. However, hyperoxia effect on body weight, kidney-to-body weight ratio, urine volume, blood urea nitrogen and glomerular filtration was not significant.
Conclusion: Although pre-treatment with hyperoxia enhances the antioxidant capacity of renal tissue and improves some of the functional and histopathological parameters of the kidney, it failed to completely restore the adverse effects of gentamicin-induced nephrotoxicity. Thus, more studies are needed to determine the clinical effect of hyperoxia on gentamicin-induced nephrotoxicity.
Materials and Methods: Twenty-eight male Wistar rats were randomly divided into four groups (n=7): Control (room air); Gentamicin (100 mg/kg, IP, for 9 days); Hyperoxia60 (60 min daily pretreatment with 95% oxygen and then 100 mg/kg gentamicin, IP, for 9 days) and Hyperoxia180 (180 min daily pretreatment with 95% oxygen and then 100 mg/kg gentamicin, IP, for 9 days). Then 24 h urine was collected and on day 10, the rats were sacrificed for serum, urine and renal tissue sampling.
Results: Results showed that hyperoxia significantly enhanced renal antioxidative capacity and decreased serum creatinine and renal failure index. Histological examination and SPECT scan also showed that tissue damage in the hyperoxia groups was lower compared to the gentamicin group. However, hyperoxia effect on body weight, kidney-to-body weight ratio, urine volume, blood urea nitrogen and glomerular filtration was not significant.
Conclusion: Although pre-treatment with hyperoxia enhances the antioxidant capacity of renal tissue and improves some of the functional and histopathological parameters of the kidney, it failed to completely restore the adverse effects of gentamicin-induced nephrotoxicity. Thus, more studies are needed to determine the clinical effect of hyperoxia on gentamicin-induced nephrotoxicity.
Type of Study: Original |
Subject:
Physiology
Received: 2023/10/31 | Accepted: 2023/11/28 | Published: 2023/12/11
Received: 2023/10/31 | Accepted: 2023/11/28 | Published: 2023/12/11
References
1. Krause KM, Serio AW, Kane TR, et al. Aminoglycosides: An Overview. Cold Spring Harb Perspect Med 2016; 6(6): a027029. [DOI]
2. Nadeem RI, Aboutaleb AS, Younis NS, et al. Diosmin Mitigates Gentamicin-Induced Nephrotoxicity in Rats: Insights on miR-21 and -155 Expression, Nrf2/HO-1 and p38- MAPK/NF-κB Pathways. Toxics 2023; 11(1): 48. [DOI]
3. Balakumar P, Rohilla A, Thangathirupathi A. Gentamicin-induced nephrotoxicity: Do we have a promising therapeutic approach to blunt it? Pharmacol Res 2010; 62(3): 179-86. [DOI]
4. Iqbal MO, Yahya EB. In vivo assessment of reversing aminoglycoside antibiotics nephrotoxicity using Jatropha mollissima crude extract. Tissue Cell 2021; 72: 101525. [DOI]
5. Huang H, Jin WW, Huang M, et al. Gentamicin-Induced Acute Kidney Injury in an Animal Model Involves Programmed Necrosis of the Collecting Duct. J Am Soc Nephrol 2020; 31(9): 2097-115. [DOI]
6. Shin HS, Yu M, Kim M, et al. Renoprotective effect of red ginseng in gentamicin-induced acute kidney injury. Lab Invest 2014; 94(10): 1147-60. [DOI]
7. Udupa V, Prakash V. Gentamicin induced acute renal damage and its evaluation using urinary biomarkers in rats. Toxicol Rep 2019; 6: 91-9. [DOI]
8. Martínez-Salgado C, Eleno N, Tavares P, et al. Involvement of reactive oxygen species on gentamicin-induced mesangial cell activation. Kidney Int 2002; 62(5): 1682-92. [DOI]
9. Nakajima T, Hishida A, Kato A. Mechanisms for protective effects of free radical scavengers on gentamicin-mediated nephropathy in rats. Am J Physiol 1994; 266(3 Pt 2): F425-31. [DOI]
10. Tomşa AM, Răchişan AL, Pandrea SL, et al. Curcumin and Vitamin C Attenuate Gentamicin-Induced Nephrotoxicity by Modulating Distinctive Reactive Species. Metabolites 2022; 13(1): 49. [DOI]
11. Mahi-Birjand M, Yaghoubi S, AbdollahpourAlitappeh M, et al. Protective effects of pharmacological agents against aminoglycoside-induced nephrotoxicity: A systematic review. Expert Opin Drug Saf 2020; 19(2): 167-86. [DOI]
12. Tähepõld P, Vaage J, Starkopf J, et al. Hyperoxia elicits myocardial protection through a nuclear factor kappaB-dependent mechanism in the rat heart. J Thorac Cardiovasc Surg 2003; 125(3): 650-60. [DOI]
13. Wahhabaghai H, Heidari R, Zeinoddini A, et al. Hyperoxia-induced preconditioning against renal ischemic injury is mediated by reactive oxygen species but not related to heat shock proteins 70 and 32. Surgery 2015; 157(6): 1014-22. [DOI]
14. Chen Z, Ding Y, Ji X, et al. Advances in Normobaric Hyperoxia Brain Protection in Experimental Stroke. Front Neurol 2020; 11: 50. [DOI]
15. Chen PW, Hsu CC, Lai LF, et al. Effects of Hypoxia-Hyperoxia Preconditioning on Indicators of Muscle Damage After Acute Resistance Exercise in Male Athletes. Front Physiol 2022; 13: 824210. [DOI]
16. Wu X, You J, Chen X, et al. An overview of hyperbaric oxygen preconditioning against ischemic stroke. Metab Brain Dis 2023; 38(3): 855-72. [DOI]
17. Tavafi M, Ahmadvand H, Tamjidipour A, et al. Effect of normobaric hyperoxia on gentamicin-induced nephrotoxicity in rats. Iran J Basic Med Sci 2014; 17(4): 287-93. [PubMed]
18. Karimi Z, Ketabchi F, Alebrahimdehkordi N, et al. Renal ischemia/reperfusion against nephrectomy for induction of acute lung injury in rats. Ren Fail 2016; 38(9): 1503-15. [DOI]
19. Pourkhalili K, Karimi Z, Farzaneh MR, et al. Renoprotective Effect of Fucoidan from Seaweed Sargassum angustifolium C. Agardh 1820 on Gentamicin-Induced Nephrotoxicity: From Marine Resources to Therapeutic Uses. Jundishapur J Nat Pharm Prod 2022; 17(2): e119081. [DOI]
20. Ali BH, Abdel Gayoum AA, Bashir AA. Gentamicin nephrotoxicity in rat: some biochemical correlates. Pharmacol Toxicol 1992; 70(6 Pt 1): 419-23. [DOI]
21. Erdem A, Gündoğan NU, Usubütün A, et al. The protective effect of taurine against gentamicin-induced acute tubular necrosis in rats. Nephrol Dial Transplant 2000; 15(8): 1175-82. [DOI]
22. Ding W, Yang L, Zhang M, et al. Chronic inhibition of nuclear factor kappa B attenuates aldosterone/salt-induced renal injury. Life Sci 2012; 90(15-16): 600-6. [DOI]
23. Jaikumkao K, Pongchaidecha A, Thongnak LO, et al. Amelioration of Renal Inflammation, Endoplasmic Reticulum Stress and Apoptosis Underlies the Protective Effect of Low Dosage of Atorvastatin in Gentamicin-Induced Nephrotoxicity. PLoS One 2016; 11(10): e0164528. [DOI]
24. Gamaan MA, Zaky HS, Ahmed HI. Gentamicin-induced nephrotoxicity: A mechanistic approach. Azhar Int J Pharm Med Sc 2023; 3(2): 11-9. [DOI]
25. Mestry SN, Gawali NB, Pai SA, et al. Punica granatum improves renal function in gentamicin-induced nephropathy in rats via attenuation of oxidative stress. J Ayurveda Integr Med 2020; 11(1): 16-23. [DOI]
26. Priyamvada S, Priyadarshini M, Arivarasu NA, et al. Studies on the protective effect of dietary fish oil on gentamicin-induced nephrotoxicity and oxidative damage in rat kidney. Prostaglandins Leukot Essent Fatty Acids 2008; 78(6): 369-81. [DOI]
27. Randjelovic P, Veljkovic S, Stojiljkovic N, et al. Gentamicin nephrotoxicity in animals: Current knowledge and future perspectives. EXCLI J 2017; 16: 388-99. [DOI]
28. Shi S, Qi Z, Ma Q, et al. Normobaric Hyperoxia Reduces Blood Occludin Fragments in Rats and Patients With Acute Ischemic Stroke. Stroke 2017; 48(10): 2848-54. [DOI]
29. Singhal AB, Wang X, Sumii T, et al. Effects of Normobaric Hyperoxia in a Rat Model of Focal Cerebral Ischemia-Reperfusion. J Cereb Blood Flow Metab 2002; 22(7): 861-8. [DOI]
30. Pourkhalili K, Hajizadeh S, Tiraihi T, et al. Ischemia and reperfusion-induced arrhythmias: role of hyperoxic preconditioning. J Cardiovasc Med (Hagerstown) 2009; 10(8): 635-42. [DOI]
31. Losada DM, Jordani ME, Jordani MC, et al. Should Preconditioning Hyperbaric Oxygenation Protect the Liver Against Ischemia-Reperfusion Injury? An Experimental Study in a Rat Model. Transplant Proc 2014; 46(1): 56-62. [DOI]
32. Rasoulian B, Kaeidi A, Pourkhodadad S, et al. Effects of pretreatment with single-dose or intermittent oxygen on Cisplatin-induced nephrotoxicity in rats. Nephrourol Mon 2014; 6(5): e19680. [DOI]
33. Pei J, Cai S, Song S, et al. Normobaric hyperoxia plays a protective role against renal ischemia-reperfusion injury by activating the Nrf2/HO-1 signaling pathway. Biochem Biophys Res Commun 2020; 532(1): 151-8. [DOI]
34. Qi Z, Yuan S, Liu KJ, et al. Normobaric hyperoxia plays a neuroprotective role after cerebral ischemia by maintaining the redox homeostasis and the level of connexin43 in astrocytes. CNS Neurosci Ther 2022; 28(10): 1509-18. [DOI]
35. Rasoulian B, Jafari M, Mahbod M, et al. Pretreatment with Oxygen Protects Rat Kidney from Cisplatin Nephrotoxicity. Ren Fail 2010; 32(2): 234-42. [DOI]
36. Tavafi M, Ahmadvand H, Tamjidipour A, et al. Effect of normobaric hyperoxia on gentamicin-induced nephrotoxicity in rats. Iran J Basic Med Sci. 2014;17(4):287-93. [PubMed]
37. Balaha MF, Alamer AA, Eisa AA, et al. Shikonin Alleviates Gentamicin-Induced Renal Injury in Rats by Targeting Renal Endocytosis, SIRT1/Nrf2/HO-1, TLR-4/NF-κB/MAPK, and PI3K/Akt Cascades. Antibiotics (Basel) 2023; 12(5): 826. [DOI]
38. Tähep ld P, Valen G, Starkopf J, et al. Pretreating rats with hyperoxia attenuates ischemia-reperfusion injury of the heart. Life Sci 2001; 68(14): 1629-40. [DOI]
39. Abouzed TK, Sherif EAE, Barakat MES, et al. Assessment of gentamicin and cisplatininduced kidney damage mediated via necrotic and apoptosis genes in albino rats. BMC Vet Res 2021; 17(1): 350. [DOI]
40. Sedaghat Z, Fatemikia H, Tanha K, et al. Evaluating the Recovery Process of Renal Ischemia/Reperfusion Injury in Rats Using Small-Animal SPECT. Iran South Med J 2019; 22(2): 77-89. (Persian) [DOI]
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