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:: Volume 20, Issue 6 (Iranian South Medical Journal 2018) ::
Iran South Med J 2018, 20(6): 519-526 Back to browse issues page
Analysis of Ala234Thr Polymorphism SEPP1 gene in Women with Breast Cancer in Guilan Province
Samaneh Mohammaddoust1, Zivar Salehi 2, Hamid Saeidi Saedi3
1- Department of Biology, School of Sciences, University of Guilan, Rasht, Iran
2- Department of Biology, School of Sciences, University of Guilan, Rasht, Iran , geneticzs@yahoo.co.uk
3- Department of Oncology, Cancer Research Center, Guilan University of Medical Sciences, Rasht, Iran
Abstract:   (3449 Views)
Background: Selenoprotein P (Sepp1) is the main selenoprotein in plasma and contains over 50% of the total plasma selenoprotein. The direct and indirect effects of Sepp1 on anti-oxidant defense can be responsible for its role in increasing cancer risk. One of the common polymorphism of SEPP1gene is the Ala234Thr single nucleotide polymorphism (SNP) in the coding region. In this research, we studied the association between SEPP1 Ala234Thr (rs3877899) polymorphism and the risk of breast cancer.
Materials and Methods: In this case-control study, 162 patients with breast cancer were compared with 227 healthy volunteers. Genomic DNA was extracted from peripheral blood leukocytes. Determination of DNA genotyping in SEPP1 Ala234Thr (rs3877899) polymorphism was performed by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using MwoI enzyme. Statistical analysis was performed using and χ2-test in the MedCalc software
Results: The genotype frequencies were significantly different between the patients and control group. Participants with AA homozygosity were at a higher risk for breast cancer compared to others (OR=3.23; 95%CI, 1.79-5.84; p=0.001).
Conclusion: The study results showed that the Ala234Thr polymorphism of the SEPP1 gene might be associated with the higher risk for breast cancer in the female population northern Iran. However, further research with larger sample size is required to confirm this research results.
Keywords: Guilan province, polymorphism, Breast cancer, Selenoprotein, Ala234Thr, SEPP1
Full-Text [PDF 806 kb]   (1100 Downloads)    
Type of Study: Original | Subject: General
Received: 2017/03/8 | Accepted: 2017/06/3 | Published: 2018/01/7
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Mohammaddoust S, Salehi Z, Saeidi Saedi H. Analysis of Ala234Thr Polymorphism SEPP1 gene in Women with Breast Cancer in Guilan Province. Iran South Med J. 2018; 20 (6) :519-526
URL: http://ismj.bpums.ac.ir/article-1-903-en.html

Volume 20, Issue 6 (Iranian South Medical Journal 2018) Back to browse issues page
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