Volume 22, Issue 4 (Iranian South Medical Journal 2019)
Iran South Med J 2019, 22(4): 191-199 |
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Edraki N, Asadollahi M, Hemmatian H, Khoshneviszadeh M, Firuzi O, Sakhteman A et al . Phenolic Content, Antioxidant Effects and Tyrosinase Inhibitory Activity of Extract of Some Stachys Species from Iran. Iran South Med J 2019; 22 (4) :191-199
URL: http://ismj.bpums.ac.ir/article-1-1142-en.html
URL: http://ismj.bpums.ac.ir/article-1-1142-en.html
Najmeh Edraki1 
, Mojtaba Asadollahi1 , Hajar Hemmatian1 , Mehdi Khoshneviszadeh2 , Omidreza Firuzi1 , Amirhossein Sakhteman3 , Amireza Jasbi * 4
, Mojtaba Asadollahi1 , Hajar Hemmatian1 , Mehdi Khoshneviszadeh2 , Omidreza Firuzi1 , Amirhossein Sakhteman3 , Amireza Jasbi * 4
1- Medicinal and Natural Products Chemistry Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
2- Medicinal and Natural Products Chemistry Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Medicinal chemistry department, School of pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
3- Medicinal chemistry department, School of pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
4- Medicinal and Natural Products Chemistry Research Center, Shiraz University of Medical Sciences, Shiraz, Iran ,jassbiar@sums.ac.ir
2- Medicinal and Natural Products Chemistry Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Medicinal chemistry department, School of pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
3- Medicinal chemistry department, School of pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
4- Medicinal and Natural Products Chemistry Research Center, Shiraz University of Medical Sciences, Shiraz, Iran ,
Abstract: (4242 Views)
Background: Given the importance of Stachys genus in Iran and their significant biological activity, we collected three species of this plant: St. aucheri Benth., Stachys benthamiana Boiss and St. inflata Benth. from different regions of Iran. We examined the free radical scavenging potential, total phenol content and tyrosinase inhibitory activity of their methanol, methanol 80% and dichloromethane extracts.
Materials and Methods: Antioxidant activity and total phenol content of the mentioned extracts were assessed using DPPH and FolinCiocalteu reagent, respectively. The inhibitory activity of the extracts on tyrosinase was also investigated.
Results: Total phenol content of different extracts was high in the extracts as 98.5-139.7 mg eq. gallic acid/g in methanol 80% extract, 36.8-54.7 mg eq. gallic acid/g in methanol extract and 17.9-44.9 mg eq. gallic acid/g in dichloromethane extract. Furthermore, their antioxidant activity was correlated with total phenol content of the extracts. Methanol 80% extract had the highest total phenol content and antioxidant activity. Evaluation of the tyrosinase inhibitory potential of the extracts revealed that St.inflata exhibited a considerable inhibitory potential (IC50= 5.4 ± 0.6 µM).
Conclusion: It can be concluded that tyrosinase inhibitory activity of Stachys genus might be due to the less polar compounds and these compounds are mostly present in the dichloromethane extract of St. inflatai over St. aucheri and St. benthamiana
Materials and Methods: Antioxidant activity and total phenol content of the mentioned extracts were assessed using DPPH and FolinCiocalteu reagent, respectively. The inhibitory activity of the extracts on tyrosinase was also investigated.
Results: Total phenol content of different extracts was high in the extracts as 98.5-139.7 mg eq. gallic acid/g in methanol 80% extract, 36.8-54.7 mg eq. gallic acid/g in methanol extract and 17.9-44.9 mg eq. gallic acid/g in dichloromethane extract. Furthermore, their antioxidant activity was correlated with total phenol content of the extracts. Methanol 80% extract had the highest total phenol content and antioxidant activity. Evaluation of the tyrosinase inhibitory potential of the extracts revealed that St.inflata exhibited a considerable inhibitory potential (IC50= 5.4 ± 0.6 µM).
Conclusion: It can be concluded that tyrosinase inhibitory activity of Stachys genus might be due to the less polar compounds and these compounds are mostly present in the dichloromethane extract of St. inflatai over St. aucheri and St. benthamiana
Type of Study: Original |
Subject:
Pharmacology
Received: 2018/07/30 | Accepted: 2019/06/2 | Published: 2019/09/25
Received: 2018/07/30 | Accepted: 2019/06/2 | Published: 2019/09/25
References
1. Ullah S, Son S, Yun HY, et al. Tyrosinase Inhibitors: A Patent Review (2011-2015). Expert Opin Ther Pat 2016; 26(3): 347-62. [DOI:10.1517/13543776.2016.1146253]
2. Chai W-M, Wei M-K, Wang R, et al. Avocado Proanthocyanidins as a Source of Tyrosinase Inhibitors: Structure Characterization, Inhibitory Activity, and Mechanism. J Agric Food Chem 2015; 63(33): 7381-7. [DOI:10.1021/acs.jafc.5b03099]
3. Karim AA, Azlan A, Ismail A, et al. Phenolic Composition, Antioxidant, Anti-Wrinkles and Tyrosinase Inhibitory Activities of Cocoa Pod Extract. BMC Complement Altern Med 2014; 14(1): 381. [DOI:10.1186/1472-6882-14-381]
4. Orhan IE, Khan MT. Flavonoid Derivatives as Potent Tyrosinase Inhibitors-A Survey of Recent Findings Between 2008-2013. Curr Top Med Chem 2014; 14(12): 1486-93. [DOI:10.2174/1568026614666140523120741]
5. Yang YF, Lai XY, Lai GY, et al. Purification and Characterization of a Tyrosinase Inhibitor from Camellia Pollen. J Func Foods 2016; 27: 140-9. [DOI:10.1016/j.jff.2016.08.056]
6. Hseu YC, Cheng KC, Lin YC, et al. Synergistic Effects of Linderanolide B Combined with Arbutin, PTU or Kojic Acid on Tyrosinase Inhibition. Curr Pharm Biotechnol 2015; 16(12): 1120-6. [DOI:10.2174/1389201016666150907112819]
7. Jamzad Z. Florestic of Iran (Lamiaceae). 1st ed. Tehran: Research Institute of Forests and Rangelands, 2012, 1066. (Persian)
8. Tundis R, Peruzzi L, Menichini F. Phytochemical and Biological Studies of Stachys Species in Relation to Chemotaxonomy: A Review. Phytochemistry 2014; 102:7-39. [DOI:10.1016/j.phytochem.2014.01.023]
9. Salmaki Y, Zarre S, Govaerts R, et al. A Taxonomic Revision of the Genus Stachys (Lamiaceae: Lamioideae) in Iran. Bot J Linn Soc 2012; 170(4): 573-617. [DOI:10.1111/j.1095-8339.2012.01317.x]
10. Jassbi AR, Miri R, Asadollahi M, et al. Cytotoxic, Antioxidant and Antimicrobial Effects of Nine Species of Woundwort (Stachys) Plants. Pharm Biol 2014; 52(1): 62-7. [DOI:10.3109/13880209.2013.810650]
11. Tavakkoli M, Miri R, Jassbi AR, et al. Carthamus, Salvia and Stachys Species Protect Neuronal Cells Against Oxidative StressInduced Apoptosis. Pharm Biol 2014; 52(12): 1550-7. [DOI:10.3109/13880209.2014.908394]
12. Namjooyan F, Azemi M, Hejazi H, et al. Antioxidant capacity and total phenolic content of Stachys aucheri endemic plant to Persia. Planta Medica 2011; 77(12): PL95. [DOI:10.1055/s-0031-1282744]
13. Kocak MS, Uren MC, Calapoglu M, et al. Phenolic Profile, Antioxidant and Enzyme Inhibitory Activities of Stachys Annua Subsp. Annua Var. Annua. S Afr J Bot 2017; 113: 128-32. [DOI:10.1016/j.sajb.2017.08.005]
14. Blois MS. Antioxidant Determinations by the Use of a Stable Free Radical. Nature 1958; 181(4617): 1199-200. [DOI:10.1038/1811199a0]
15. Singleton VL, Rossi JA. Colorimetry of Total Phenolics with PhosphomolybdicPhosphotungstic Acid Reagents. Am J Enol Viticul 1965; 16(3): 144-58.
16. Zarrabi MM, Asghari B, Maryamabadi A, et al. Phytochemical Properties and Inhibitory and Antioxidant Effects of the Decoction, Infusion and Hydro-Alcoholic Extract of Nepeta Racemosa on α-Amylase and α-Glucosidase. Iran South Med J 2019; 22(2): 90-105. (Persian) [DOI:10.29252/ismj.22.2.90]
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