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:: Volume 24, Issue 1 (Iranian South Medical Journal 2021) ::
Iran South Med J 2021, 24(1): 27-45 Back to browse issues page
Antibacterial Activity of Tunichrome Released from phallusia nigra Marine Tunicate Obtained from Bushehr Coast
Zohre Marhamati1 , Mohammad Hossein Marhamatizadeh 2, Gholam Hossein Mohebbi3
1- Department of Food Hygiene, School of Veterinary Medicine, Islamic Azad University, Kazerun Branch, Kazerun, Iran
2- Department of Food Hygiene, School of Veterinary Medicine, Islamic Azad University, Kazerun Branch, Kazerun, Iran , drmarhamati@gmail.com
3- The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Insti-tute, Bushehr University of Medical Sciences, Bushehr, Iran
Abstract:   (1167 Views)
Background: Marine life has long been used for their bioactive compounds. Several amazing compounds with different biological activities have so far been identified in tunicates. The aim of the current study was to evaluate the antimicrobial activity of tunichrome released from phallusia nigra marine tunicates collected from Bushehr province coasts, against four strains of Staphylococcus aureus, Bacillus cereus, Salmonella enterica, and Escherichia coli.
Materials and Methods: Thirty samples of phallusia nigra marine tunicates were randomly collected from Nayband Bay in Bushehr province, and then their tunichrome was extracted and lyophilized. The antimicrobial activity (MIC and MBC) of the tunichrome extract was evaluated by agar well diffusion and broth macrodilution methods against four strains of foodborne pathogens. Chemical composition of methanol: chloroform:n-hexane extract of the tunichrome was detected by Gas Chromatography-Mass Spectrometry (GC-MS).
Results: According to the results, the tunichrome extract showed significant inhibitory and lethal activities against all four studied bacterial strains. However, its antimicrobial activity was higher against Bacillus cereus and Salmonella enterica. The presence of antimicrobial compounds detected by GC-MS confirmed the results of the antibacterial activity of the tunichrome.
Conclusion: According to the results, the tunichrome released from phallusia nigra marine tunicate can be an appropriate marine source of antimicrobial compounds with significant performance against foodborne pathogens. According to the literature, the secondary metabolites in the tunichrome have potential biological and neutraceutical effects which require more laboratory studies.
Keywords: Marine tunicate, phallusia nigra, Tunichrome, Antimicrobial activity, Secondary metabolite, Persian Gulf
Full-Text [PDF 630 kb]   (285 Downloads)    
Type of Study: Original | Subject: General
Received: 2020/10/6 | Accepted: 2021/01/25 | Published: 2021/02/27
1. Palanisamy SK, Rajendran NM, Marino A. Natural Products Diversity of Marine Ascidians (Tunicates; Ascidiacea) And Successful Drugs In Clinical Development. Nat Prod Bioprospect 2017; 7(1): 1-111. [DOI:10.1007/s13659-016-0115-5]
2. Khodadadipour T, Amini K, Mahmoudi R. Evaluation of Virulence And Enterotoxin Genes In Salmonella Enteritidis Strains Isolated From Meat And Egg Samples By Multiplex-PCR. J Food Microbiol 2016; 3(2): 25-33. (Persian)
3. Kaper JB, Nataro JP, Mobley HL. Pathogenic Escherichia Coli. Nat Rev Microbiol 2004; 2(2): 123-40. [DOI:10.1038/nrmicro818]
4. Schoeni JL, Lee Wong AC. Bacillus Cereus Food Poisoning And Its Toxins. J Food Prot 2005; 68(3): 636-48. [DOI:10.4315/0362-028X-68.3.636]
5. Le Loir Y, Baron F, Gautier M. Staphylococcus Aureus And Food Poisoning. Genet Mol Res 2003; 2(1): 63-76.
6. Blair JM, Webber MA, Baylay AJ, et al. Molecular Mechanisms of Antibiotic Resistance. Nat Rev Microbiol 2015; 13(1): 42-51. [DOI:10.1038/nrmicro3380]
7. Barmak A, Niknam K, Mohebbi G, et al. Antibacterial Studies of Hydroxyspiro [Indoline-3, 9-Xanthene] Trione Against Spiro [Indoline3, 9-Xanthene] Trione And Their Use As Acetyl And Butyrylcholinesterase Inhibitors. Microb Pathog 2019; 130: 95-9. [DOI:10.1016/j.micpath.2019.03.002]
8. Mohebbi GH, Nabipour I, Vazirizadeh A. The Sea, The Future Pharmacy. Iran South Med J 2014; 17(4): 748-88. (Persian)
9. Jha RK, Zi-Rong X. Biomedical Compounds From Marine Organisms. Mar Drugs 2004; 2(3): 123-46. [DOI:10.3390/md203123]
10. Boziaris IS. Food Ingredients From The Marine Environment. Marine Biotechnology Meets Food Science And Technology. Front Mar Sci 2014; 1(66): 1-4. [DOI:10.3389/fmars.2014.00066]
11. Blunt JW, Carroll AR, Copp BR, et al. Marine Natural Products. Nat Prod Rep 2018; 35(1): 8-53. [DOI:10.1039/C7NP00052A]
12. Costantino V, Fattorusso E, Menna M, et al. Chemical Diversity of Bioactive Marine Natural Products: An Illustrative Case Study. Curr Med Chem 2004; 11(13): 1671-92. [DOI:10.2174/0929867043364973]
13. Galinier R, Roger E, Sautiere PE, et al. Halocyntin And Apillosin, Two New Antimicrobial Peptides Isolated From Hemocytes of The Solitary Tunicate, Halocynthia Papillosa. J Pept Sci 2009; 15(1): 48-55. [DOI:10.1002/psc.1101]
14. Shenkarev ZO, Panteleev PV, Balandin SV, et al. Recombinant Expression And Solution Structure of Antimicrobial Peptide Aurelin From Jellyfish Aurelia Aurita. Biochemi Biophys Res Commun 2012; 429(1-2): 63-9. [DOI:10.1016/j.bbrc.2012.10.092]
15. Holand LZ. Tunicates. Curr Biol 2016; 26(4): 146-52. [DOI:10.1016/j.cub.2015.12.024]
16. Lambert G, Karney RC, Rhee WY, et al. Wild And Cultured Edible Tunicates: A Review. Manag Biol Invasions 2016; 7(1): 59-66. [DOI:10.3391/mbi.2016.7.1.08]
17. Mohebbi GH, Arshadi SS, Nabipour I, et al. Marine Tunicate, The Electuary of Mithridates. Iran South Med J 2015; 18(4): 845-97. (Persian)
18. Cai M, Sugumaran M, Robinson WE. The Crosslinking And Antimicrobial Properties of Tunichrome. Comp Biochem Physiol B Biochem Mol Biol 2008; 151(1): 110-7. [DOI:10.1016/j.cbpb.2008.06.004]
19. Sugumaran M, Robinson WE. Structure, Biosynthesis And Possible Function of Tunichromes And Related Compounds. Comp Biochem Physiol B Biochem Mol Biol 2012; 163(1): 1-25. [DOI:10.1016/j.cbpb.2012.05.005]
20. Mehrdost M, Kamrani E, Owfi F. Occurrence Phallusia Nigra Savigny, 1816 (Tunicata: Ascidiacea) From Intertidal Hengam Island (Persian Gulf, Iran). J Anim Environ 2015; 7(3): 175-8. (Persian)
21. Najafi A, Amini Khoei Z, Tajbakhsh S, et al. Evaluation of The Activity of New Species of Jelly Fish Collected From Nayband Bay In Bushehr Against Human Pathogenic Bacteria. J Anim Physiol Develop (Quarterly J Biol Sci) 2016; 9(4): 35-42. (Persian)
22. Tajbakhsh S, Barmak A, Vakhshiteh F, et al. In Vitro Antibacterial Activity of The Prosopis Juliflora Seed Pods On Some Common Pathogens. J Clin Diagn Res 2015; 9(8): 13-5. [DOI:10.7860/JCDR/2015/13549.6370]
23. Mohebbi GH, Vatanpour H, Vazirizadeh A, et al. Phospholipase A2 Activity of The Persian Gulf Upside-Down Jellyfish Venom (Cassiopea Andromeda). Iran South Med J 2017; 20(3): 287-300. (Persian)
24. CLSI. Clinical And Laboratory Standard Institute. Performance Standards For Antimicrobial Disk Susceptibility Testing: Approved Standard: National Committee For Clinical Laboratory Standards 2012; 29(1): 1-76.
25. Rufchaie R, Mirvaghefi A, Hoseinifar SH, et al. Anti-Microbial Activity of Echhornia Crasippes Aquatic And Hydromethanolic Leaves Extract. J Fish 2018; 71(1): 31-41. (Persian)
26. Tajbakhsh S, Pouyan M, Zandi K, et al. In Vitro Study of Antibacterial Activity of The Alga Sargassum Oligocystum From The Persian Gulf. Eur Rev Med Pharmacol Sci 2011; 15(3): 293-8.
27. Olano C, Méndez C, Salas JA. Antitumor Compounds From Marine Actinomycetes. Mar Drugs 2009; 7(2): 210-48. [DOI:10.3390/md7020210]
28. Manivasagan P, Venkatesan J, Sivakumar K, et al. Pharmaceutically Active Secondary Metabolites of Marine Actinobacteria. Microbiol Res 2014; 169(4): 262-78. [DOI:10.1016/j.micres.2013.07.014]
29. Nabipour I, Moradi M, Mohebbi GH. A First Record On Population of The Alien Venomous Jellyfish, Cassiopea Andromeda (Forsskal, 1775)(Cnidaria: Scyphozoa: Rhizostomea) In The Nayband Lagoon From Bushehr-Iran (Persian Gulf). J Chem Pharm Res 2015; 7(3): 1710-3.
30. Ravikumar S, Gnanadesigan M, Saravanan A, et al. Antagonistic Properties of Seagrass Associated Streptomyces Sp. RAUACT-1: A Source For Anthraquinone Rich Compound. Asian Pac J Trop Med 2012; 5(11): 887-90. [DOI:10.1016/S1995-7645(12)60165-5]
31. Carte BK. Biomedical Potential of Marine Natural Products. Bioscience 1996; 46(4): 271-86. [DOI:10.2307/1312834]
32. Davidson BS. Ascidians: Producers of Amino Acid Derived Metabolites. Chem Rev 1993; 93(5): 1771-91. [DOI:10.1021/cr00021a006]
33. Karthi S, Somanath B, Abdul Jaffar AH. Efficacy of Methanolic Extract of A Marine Ascidian, Lissoclinum Bistratum For Antimicrobial Activity. J Chem Biol Phys Sci 2015; 5(4): 4119-25.
34. Ananthan G, Iyappan K. Investigation of Antibacterial Potential of Ascidian, Microcosmus Exasperatus (Heller, 1878) Against Human Urinary Tract Patogens. World J Pharm Pharm Sci 2013; 3(1): 396-403.
35. Selva Prabhu A, Ananthan G, Mohamed Hussain HS, et al. Antibacterial Activity of Ascidian Phallusia Arabica Against Human Clinical Isolates. J Appl Pharm Sci 2011; 1(10): 143-5.
36. Sivaperumal P, Ananthan G, Hussain SM. Exploration of Antibacterial Effects On The Crude Extract of Marine Ascidian Aplidium Multiplicatum Against Clinical Isolates. Int J Med Med Sci 2010; 2(12): 382-6.
37. Bell W, Mitchell R. Chemotactic And Growth Responses of Marine Bacteria To Algal Extracellular Products. Biol Bull 1972; 143(2): 265-77. [DOI:10.2307/1540052]
38. Chouhan S, Sharma K, Guleria S. Antimicrobial Activity of Some Essential Oils-Present Status And Future Perspectives. Medicines 2017; 4(3): 58. [DOI:10.3390/medicines4030058]
39. Bozin B, Mimica-Dukic N, Samojlik I, et al. Antimicrobial And Antioxidant Properties of Rosemary And Sage (Rosmarinus Officinalis L. And Salvia Officinalis L., Lamiaceae) Essential Oils. J Agric Food Chem 2007; 55(19): 7879-85. [DOI:10.1021/jf0715323]
40. Sarhadizadeh N, Afkhami M, Ehsanpour M. Evaluation of Antibacterial, Antifungal And Cytotoxic Agents of Ascidian Phallusia Nigra (Savigny, 1816) From Persian Gulf. Eur J Exp Biol 2014; 4(1): 250-3.
41. Sindhu TJ, Arikkatt SD, Vincent G, et al. Biological Activities of Oxazine And Its Derivatives: A Review. Int J Pharm Sci Res 2013; 4(11): 134-43.
42. Hamza A, Elsayed HA, Assy MG, et al. Synthesis And Antimicrobial Activity of Some New Triazine, 1,3-Oxazine, Fused Pyridine And Pyrimidine Derivatives. World Appl Sci J 2018; 36(5): 637-45.
43. Mathew BP, Kumar A, Sharma S, et al. An Eco-Friendly Synthesis And Antimicrobial Activities of Dihydro-2H-Benzo-And Naphtho-1,3-Oxazine Derivatives. Eur J Med Chem 2010; 45(4): 1502-7. [DOI:10.1016/j.ejmech.2009.12.058]
44. Sunil D, Upadhy SH, Murugappan R. Synthesis, Characterization And QSAR Studies of Some New 1,3-Oxazines As Potent Antimicrobial Agents. Res J Pharm Sci 2013; 2(2): 15-9.
45. Trombetta D, Saija A, Bisignano G, et al. Study On The Mechanisms of The Antibacterial Action of Some Plant Alpha,Beta -Unsaturated Aldehydes. Lett Appl Microbiol 2002; 35(4): 285-90. [DOI:10.1046/j.1472-765X.2002.01190.x]
46. Bisignano G, Laganà MG, Trombetta D, et al. In Vitro Antibacterial Activity of Some Aliphatic Aldehydes From Olea Europaea L. FEMS Microbiol Lett 2001; 198(1): 9-13. [DOI:10.1111/j.1574-6968.2001.tb10611.x]
47. Kovanda L, Zhang W, Wei X, et al. In Vitro Antimicrobial Activities of Organic Acids And Their Derivatives On Several Species of Gram-Negative And Gram-Positive Bacteria. Molecules 2019; 24(20): 3770. [DOI:10.3390/molecules24203770]
48. Badshah SL, Naeem A. Bioactive Thiazine And Benzothiazine Derivatives: Green Synthesis Methods And Their Medicinal Importance. Molecules 2016; 21(8): 1054. [DOI:10.3390/molecules21081054]
49. Sharma PK, Makkar R. A Review: Thiazines Derivatives Treated As Potential Antimicrobial Agents. Asian J Pharm Clin Res 2017; 10(1): 43-6. [DOI:10.22159/ajpcr.2017.v10i1.115467]
50. Shweta S, Pandeya SN, Deepika Y, et al. Synthesis And Biological Activity of Phenothiazine Derivatives. Int J Res Ayurveda Pharm 2011; 2(4): 1130-7.
51. Deshmukh R. Synthesis, Structural Study And Biological Evaluation of 1,3-Thiazine. Pelagia Res Lib Der Chem Sin 2015; 6(3): 59-63.
52. Smith RE. Updated By Staff. Azine Dyes. In: Kirk-Othmer, editors. Encyclopedia of Chemical Technology. New York: John Wiley & Sons Inc, 2013, 1-10. [DOI:10.1002/0471238961.0126091419130920.a01.pub2]
53. Govindan S, Valliappan R, Chakravarthy J, et al. Synthesis, Characterization And Biological Studies of Some 3,5-Diaryl-Tetrahydro-NFormyl-1,4-Thiazine-1,1-Dioxide. J Chem Pharm Res 2013; 5(1): 99-103.
54. Bansode TN, Shelke JV, Dongre VG. Synthesis And Antimicrobial Activity of Some New N-Acyl Substituted Phenothiazines. Eur J Med Chem 2009; 44(12): 5094-8. [DOI:10.1016/j.ejmech.2009.07.006]
55. Varsha KK, Devendra L, Shilpa G, et al. 2,4- Di-Tert-Butyl Phenol As The Antifungal, Antioxidant Bioactive Purified From A Newly Isolated Lactococcus sp. Int J Food Microbiol 2015; 211: 44-50. [DOI:10.1016/j.ijfoodmicro.2015.06.025]
56. Huang CB, Alimova Y, Myers TM, et al. Short-And Medium-Chain Fatty Acids Exhibit Antimicrobial Activity For Oral Microorganisms. Arch Oral Biol 2011; 56(7): 650-4. [DOI:10.1016/j.archoralbio.2011.01.011]
57. Shim J, Jyothi NR, Farook NM. Biological Applications of Thiosemicarbazones And Their Metal Complexes. Asian J Chem 2013; 25(10): 5838-40. [DOI:10.14233/ajchem.2013.OH105]
58. Perez-Rebolledo A, Teixeira LR, Batista AA, et al. 4-Nitroacetophenone-Derived Thiosemicarbazones And Their Copper (II) Complexes With Significant In Vitro Anti-Trypanosomal Activity. Eur J Med Chem 2008; 43(5): 939-48. [DOI:10.1016/j.ejmech.2007.06.020]
59. Da Silva LL, Nascimento M, Silva DHS, et al. Antibacterial Activity of A Stearic Acid Derivative From Stemodia Foliosa. Planta Med 2002; 68(12): 1137-9. [DOI:10.1055/s-2002-36346]
60. Hameed IH, Altameme HJ, Idan SA. Artemisia Annua: Biochemical Products Analysis of Methanolic Aerial Parts Extract And Anti-Microbial Capacity. Res J Pharm Biol Chem Sci 2016; 7(2): 1843-68.
61. Dogan A, Otlu S, Celebi O, et al. An Investigation of Antibacterial Effects of Steroids. Turk J Vet Anim Sci 2017; 41(2): 302-5. [DOI:10.3906/vet-1510-24]
62. Hussein AO, Mohammed GJ, Hadi MY, et al. Phytochemical Screening of Methanolic Dried Galls Extract of Quercus Infectoria Using Gas Chromatography-Mass Spectrometry (GC-MS) And Fourier Transform-Infrared (FT-IR). J Pharmacognosy Phytother 2016; 8(3): 49-59. [DOI:10.5897/JPP2015.0368]
63. Xiang L, Yi X, Wang Y, et al. Antiproliferative And Anti-Inflammatory Polyhydroxylated Spirostanol Saponins From Tupistra Chinensis. Sci Rep 2016; 6: 31633. [DOI:10.1038/srep31633]
64. Shen P, Wang SL, Liu XK, et al. Steroidal Saponins From Rhizomes of Tupistra Wattii HOOK. F. Chem Pharm Bull 2003; 51(3): 305-8. [DOI:10.1248/cpb.51.305]
65. Raju J, Mehta R. Cancer Chemopreventive And Therapeutic Effects of Diosgenin, A Food Saponin. Nutr Cancer 2009; 61(1): 27-35. [DOI:10.1080/01635580802357352]
66. Wang YF, Li XC, Yang HY, et al. Inhibitory Effects of Some Steroidal Saponins On Human Spermatozoa In Vitro. Planta Med 1996; 62(2): 130-2. [DOI:10.1055/s-2006-957834]
67. Niwa A, Takeda O, Ishiwara M, et al. Screening Test For Platelet Aggregation Inhibitor In Natural Products. The Active Principle of Anemarrhenae Rhizoma. Yakugaku Zasshi 1988; 108(6): 555-61. [DOI:10.1248/yakushi1947.108.6_555]
68. Nakashima N, Kimura I, Kimura M, et al. Isolation of Pseudoprototimosaponin AIII From Rhizomes of Anemarrhena asphodeloides And Its Hypoglycemic Activity In StreptozotocinInduced Diabetic Mice. J Nat Prod 1993; 56(3): 345-50. [DOI:10.1021/np50093a006]
69. Marston A, Hostettmann K. Review Article Number 6: Plant Molluscicides. Phytochemistry 1985; 24(4): 639-52. [DOI:10.1016/S0031-9422(00)84870-0]
70. Takeda O, Tanaka S, Yamasaki K, et al. Screening For Molluscicidal Activity In Crude Drugs. Chem Pharm Bull 1989; 37(4): 1090-1. [DOI:10.1248/cpb.37.1090]
71. Rahman A, Choudhary MI, Asif F, et al. Microbial Transformation of Sarsasapogenin By Fusarium Lini. Phytochemistry 1998; 49(8): 2341-2. [DOI:10.1016/S0031-9422(98)00403-8]
72. Podolak I, Galanty A, Sobolewska D. Saponins As Cytotoxic Agents: A Review. Phytochem Rev 2010; 9(3): 425-74. [DOI:10.1007/s11101-010-9183-z]
73. Kanmoto T, Mimaki Y, Sashida Y, et al. Steroidal Constituents From The Underground Parts of Reineckea Carnea And Their Inhibitory Activity On cAMP Phosphodiesterase. Chem Pharm Bull 1994; 42(4): 926-31. [DOI:10.1248/cpb.42.926]
74. Eggert H, Djerassi C. 13C NMR Spectra of Sapogenins. Tetrahedron Lett 1975; 16(42): 3635-8. [DOI:10.1016/S0040-4039(00)91344-3]
75. Choi ND, Zeng J, Choi BD, et al. Shelf Life of Bottled Sea Squirt Halocynthia Roretzi Meat Packed In Vegetable Oil (BSMO). Fish Aquat Sci 2014; 17(1): 37-46. [DOI:10.5657/FAS.2014.0037]
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Marhamati Z, Marhamatizadeh M H, Mohebbi G H. Antibacterial Activity of Tunichrome Released from phallusia nigra Marine Tunicate Obtained from Bushehr Coast. Iran South Med J. 2021; 24 (1) :27-45
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