[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 11, Issue 2 (Iranian South Medical Journal 2009) ::
Iran South Med J 2009, 11(2): 108-115 Back to browse issues page
Screening the products of Streptomyces using cell culture technique to detect antitumor agents
Saeed Tajbakhsh Mr 1, Sepehr Salehi Mr, Nasrin Moazami Ms
1- , tajbakhshsaeed@yahoo.com
Abstract:   (16786 Views)
Background: Finding new antitumor compounds is essential for treating cancer. Streptomyces species are the major producers of antitumor agents. The objective of the present study was to screen the products of soil isolated Streptomyces species using cell culture technique to detect antitumor agents. Methods: Growth curve of Streptomyces was determined. In the production process, spore suspensions were inoculated to preculture medium and after 48 hours incubation, the bacteria were transfered to production medium. After 120 hours incubation of production medium, the process was stopped and fermentation broths were obtained. "Differential agar diffusion bioassay for cytotoxic substances", as a technique, was performed for the screening of antitumor compounds, so that the fermentation broths were examined on mouse T cell lymphoma using the mentioned technique. Cytotoxicity effect on T cell lymphoma was detected with appearance of blue zone on the cultures containing cancerous cells. Results: Two out of 125 products of Streptomyces showed positive result (blue zone). Conclusion: Two soil isolated Streptomyces products exhibited cytotoxic activity against tumor cell line. Therefore, these two products may have antitumor properties which warrant the need of further investigations.
Keywords: streptomyses, antitumor, cancer, cytotoxic
Full-Text [PDF 204 kb]   (1281 Downloads)    
Type of Study: Original | Subject: General
Received: 2009/05/16 | Accepted: 2009/05/16 | Published: 2009/05/16
Send email to the article author

Add your comments about this article
Your username or Email:


XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

tajbakhsh S, Salehi S, Moazami N. Screening the products of Streptomyces using cell culture technique to detect antitumor agents. Iran South Med J. 2009; 11 (2) :108-115
URL: http://ismj.bpums.ac.ir/article-1-145-en.html

Volume 11, Issue 2 (Iranian South Medical Journal 2009) Back to browse issues page
دانشگاه علوم پزشکی بوشهر، طب جنوب ISMJ

Iranian South Medical Journal is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License which allows users to read,
copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly

Copyright © 2017, Iranian South Medical Journal| All Rights Reserved

Persian site map - English site map - Created in 0.05 seconds with 32 queries by YEKTAWEB 4162