Volume 28, Issue 2 (Iran South Med J 2025)
Iran South Med J 2025, 28(2): 599-610 |
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Zad F, Nazari R, Aghaee S S. Molecular Investigation of AdeIJK Efflux Pump Genes in Extensively Drug Resistant Acinetobacter Baumannii and their Associa-tion with Antimicrobial Resistance Patterns. Iran South Med J 2025; 28 (2) :599-610
URL: http://ismj.bpums.ac.ir/article-1-2271-en.html
URL: http://ismj.bpums.ac.ir/article-1-2271-en.html
1- Department of Microbiology, Qom Branch, School of Science and New Technologies, Islamic Azad University, Qom, Iran
2- Department of Microbiology, Qom Branch, School of Science and New Technologies, Islamic Azad University, Qom, Iran ,Razieh.nazari@iau.ac.ir
2- Department of Microbiology, Qom Branch, School of Science and New Technologies, Islamic Azad University, Qom, Iran ,
Abstract: (10 Views)
Background: Acinetobacter baumannii causes numerous therapeutic problems in patients due to the presence of various effective factors that facilitate antibiotic resistance such as efflux pumps, beta-lactamase and the ability to form a strong biofilm. The aim of this study was to investigate the presence of AdeIJK efflux pump genes in clinical isolates of A. baumannii with extensive drug resistance and evaluation of its relationship with antimicrobial resistance.
Materials and Methods: This descriptive cross-sectional study examined 81 clinical isolates of A. baumannii with extensive drug resistance for the presence of adeI, adeJ, and adeK genes using PCR. Then, the relationship between the presence of each of these genes and the antimicrobial resistance of the isolates was evaluated. The results were analyzed using Pearson's chi-squared test in SPSS software (Version 16).
Results: In the present study, the rates of resistance to polymyxin, colistin and tobramycin were observed to be 15.6%, 42.74%, and 48.9%, respectively. Among 81 extensively drug-resistant A. baumannii isolates, 69 isolates (85.18%) carried adeI gene, 76 isolates (93.82%) carried adeJ gene, 71 isolates (87.65%) carried adeK gene. Moreover, 95% of the isolates had at least two genes from the AdeIJK efflux pump, which is consistent with the extensively drug-resistant
phenotype of the isolates.
Conclusion: The results showed that more than 97% of Acinetobacter baumannii isolates with extensive drug resistance were resistant to most antibiotics and more than 80% of them also had AdeIJK pump genes. Therefore, adopting appropriate strategies for the control and treatment of hospital infections is essential to prevent the spread of these genes.
Materials and Methods: This descriptive cross-sectional study examined 81 clinical isolates of A. baumannii with extensive drug resistance for the presence of adeI, adeJ, and adeK genes using PCR. Then, the relationship between the presence of each of these genes and the antimicrobial resistance of the isolates was evaluated. The results were analyzed using Pearson's chi-squared test in SPSS software (Version 16).
Results: In the present study, the rates of resistance to polymyxin, colistin and tobramycin were observed to be 15.6%, 42.74%, and 48.9%, respectively. Among 81 extensively drug-resistant A. baumannii isolates, 69 isolates (85.18%) carried adeI gene, 76 isolates (93.82%) carried adeJ gene, 71 isolates (87.65%) carried adeK gene. Moreover, 95% of the isolates had at least two genes from the AdeIJK efflux pump, which is consistent with the extensively drug-resistant
phenotype of the isolates.
Conclusion: The results showed that more than 97% of Acinetobacter baumannii isolates with extensive drug resistance were resistant to most antibiotics and more than 80% of them also had AdeIJK pump genes. Therefore, adopting appropriate strategies for the control and treatment of hospital infections is essential to prevent the spread of these genes.
Type of Study: Original |
Subject:
Bacteriology
Received: 2025/07/13 | Accepted: 2025/09/28 | Published: 2025/11/22
Received: 2025/07/13 | Accepted: 2025/09/28 | Published: 2025/11/22
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