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:: Volume 19, Number 2 (Iranian South Medical Journal 2016) ::
Iran South Med J 2016, 19(2): 296-305 Back to browse issues page
Evaluation of the expansion of umbilical cord blood derived from CD133+ cells on biocompatible microwells
Mina Soufizomorrod1, Masoud Soleimani *2, Saeaid Abroun1, Majid Mossahebi Mohammadi1, Gholamreza Khamisipour3, Naser Mobarra4, Sadegh Babashah5
1- Department of Hematology, School of Medicine, Tarbiat Modares University, Tehran, Iran
2- Department of Hematology, School of Medicine, Tarbiat Modares University, Tehran, Iran , soleim_m@modares.ac.ir
3- Department of Allied Medicine, School of Para Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
4- Department of Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
5- Department of Genetics, School of Biological Sciences, Tarbiat Modares University, Tehran, Iran
Abstract:   (1348 Views)

Background: Hematopoietic stem cell transplantation (HSCT) is a therapeutic approach for treatment of hematological malignancies and incompatibility of Bone marrow. Umbilical cord blood (UCB) has known as an alternative for hematopoietic stem/progenitor cells (HPSC) in allogeneic transplantation. The low volume of collected samples is the main hindrance in application of HPSC derived from umbilical cord blood. So, ex vivo expansion of HPSCs is the useful approach to overcome this restriction. The goal of using this system is to produce appropriate amount of hematopoietic stem cells, which have the ability of transplantation and long term haematopoiesis.

Material & Methods: In current study CD133+ cells were isolated from cord blood (CB). Isolated cells were seeded on microwells. Then expanded cells proliferation rate and ability in colony formation were assessed and finally were compared with 2 Dimensional (2D) culture systems.

Results: Our findings demonstrated that CD133+ cells derived from UCB which were cultivated on microwells had significantly higher rate of proliferation in compared with routine cell culture systems.

Conclusion: In Current study, it was shown that CD133+ cells’ proliferations which were seeded on PDMS microwells coated with collagen significantly increased. We hope that 3 dimensional (3D) microenvironment which mimics the 3D structure of bone marrow can solve the problem of using UCB as an alternative source of bone marrow.

Keywords: Umbilical Cord Blood, CD133+ cells, 3 Dimensional microenvironment, PDMS
Full-Text [PDF 733 kb]   (393 Downloads)    
Type of Study: Original | Subject: Biochemistry. Cell Biology and Genetics
Received: 2016/05/17 | Accepted: 2016/05/17 | Published: 2016/05/17
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DOI: 10.18869/acadpub.ismj.19.2.296
PMID: 24505514
PMCID: PMC3913131


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Soufizomorrod M, Soleimani M, Abroun S, Mossahebi Mohammadi M, Khamisipour G, Mobarra N et al . Evaluation of the expansion of umbilical cord blood derived from CD133+ cells on biocompatible microwells. Iran South Med J. 2016; 19 (2) :296-305
URL: http://ismj.bpums.ac.ir/article-1-792-en.html
Volume 19, Number 2 (Iranian South Medical Journal 2016) Back to browse issues page
دانشگاه علوم پزشکی بوشهر، طب جنوب ISMJ

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